Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2017.07.003 http://hdl.handle.net/11449/163366 |
Resumo: | Background: Photodynamic therapy (PDT) has been established in several countries as an alternative therapy for the treatment of various malignancies. This therapy involves the incorporation of a photosensitizer (PS) that is activated by visible light and form reactive oxygen species leading to target cell death by apoptosis or necrosis. Previously, our group has demonstrated that CHL-T (semi-synthesized from chlorophyll a and containing a linked solubilizing group TRISMA (R)) presented a pronounced potential to induce death in HeLa cell line after PDT. In the present study, besides confirm the high cytotoxicity in another cell line, we have further investigated the cell death mechanisms caused by CHL-T as a photosensitizer in laryngeal carcinoma cells. Methods: Cells were exposed to different concentrations of three photosensitizers, namely, hypericin (HY), unmodified chlorin (CHL) and a synthesized amphiphilic chlorin derivative (CHL-T). PSs accumulation and localization were accessed by fluorescence assays. Photosensitization was induced at 6 J cm(-2) using red LEDs (630 +/- 10 nm). Viability was assessed by mitochondrial function (MTT); whereas apoptosis/necrosis was evaluated by fluorescence microscopy and flow cytometry. Expression of pro-apoptotic p53 protein was studied by Western blot. Results and conclusions: All PS showed similar localization profile in the HEp-2 cells. The use of CHL-T increased the percentage of apoptotic cells and also p53 expression in comparison with the use of HY and CHL as photosensitizers. This study shows a significant effect of CHLT associated with red light (630 +/- 10 nm and 18 mW cm(-2)) irradiation on a cancer cell line, indicating the potential of this amphiphilic chlorin in enhancing the therapeutic effectiveness of Photodynamic Therapy (PDT). |
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Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizerAmphiphilic chlorinPhotodynamic therapyTumor cellsPhototoxicityApoptosisp-53Background: Photodynamic therapy (PDT) has been established in several countries as an alternative therapy for the treatment of various malignancies. This therapy involves the incorporation of a photosensitizer (PS) that is activated by visible light and form reactive oxygen species leading to target cell death by apoptosis or necrosis. Previously, our group has demonstrated that CHL-T (semi-synthesized from chlorophyll a and containing a linked solubilizing group TRISMA (R)) presented a pronounced potential to induce death in HeLa cell line after PDT. In the present study, besides confirm the high cytotoxicity in another cell line, we have further investigated the cell death mechanisms caused by CHL-T as a photosensitizer in laryngeal carcinoma cells. Methods: Cells were exposed to different concentrations of three photosensitizers, namely, hypericin (HY), unmodified chlorin (CHL) and a synthesized amphiphilic chlorin derivative (CHL-T). PSs accumulation and localization were accessed by fluorescence assays. Photosensitization was induced at 6 J cm(-2) using red LEDs (630 +/- 10 nm). Viability was assessed by mitochondrial function (MTT); whereas apoptosis/necrosis was evaluated by fluorescence microscopy and flow cytometry. Expression of pro-apoptotic p53 protein was studied by Western blot. Results and conclusions: All PS showed similar localization profile in the HEp-2 cells. The use of CHL-T increased the percentage of apoptotic cells and also p53 expression in comparison with the use of HY and CHL as photosensitizers. This study shows a significant effect of CHLT associated with red light (630 +/- 10 nm and 18 mW cm(-2)) irradiation on a cancer cell line, indicating the potential of this amphiphilic chlorin in enhancing the therapeutic effectiveness of Photodynamic Therapy (PDT).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Programa Posgrad Interunidades Bioengn EESC FMRP, Sao Carlos, SP, BrazilUniv Estadual Paulista Mesquita Filho, Dept Diagnost & Cirurgia, Araraquara, SP, BrazilUniv Fed Sao Carlos, Dept Quim, Sao Carlos, SP, BrazilUniv Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP, BrazilUniv Estadual Paulista Mesquita Filho, Dept Diagnost & Cirurgia, Araraquara, SP, BrazilFAPESP: 2015/21110-4FAPESP: 2013/07276-1Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Moritz, Milene N. O.Rossa Junior, Carlos [UNESP]Oliveira, Kleber T. deUliana, Marciana P.Perussi, Janice R.2018-11-26T17:41:42Z2018-11-26T17:41:42Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article355-362application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2017.07.003Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 19, p. 355-362, 2017.1572-1000http://hdl.handle.net/11449/16336610.1016/j.pdpdt.2017.07.003WOS:000412252100053WOS000412252100053.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis And Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2023-11-12T06:14:18Zoai:repositorio.unesp.br:11449/163366Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:30:40.121217Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
title |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
spellingShingle |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer Moritz, Milene N. O. Amphiphilic chlorin Photodynamic therapy Tumor cells Phototoxicity Apoptosis p-53 |
title_short |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
title_full |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
title_fullStr |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
title_full_unstemmed |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
title_sort |
Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer |
author |
Moritz, Milene N. O. |
author_facet |
Moritz, Milene N. O. Rossa Junior, Carlos [UNESP] Oliveira, Kleber T. de Uliana, Marciana P. Perussi, Janice R. |
author_role |
author |
author2 |
Rossa Junior, Carlos [UNESP] Oliveira, Kleber T. de Uliana, Marciana P. Perussi, Janice R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Moritz, Milene N. O. Rossa Junior, Carlos [UNESP] Oliveira, Kleber T. de Uliana, Marciana P. Perussi, Janice R. |
dc.subject.por.fl_str_mv |
Amphiphilic chlorin Photodynamic therapy Tumor cells Phototoxicity Apoptosis p-53 |
topic |
Amphiphilic chlorin Photodynamic therapy Tumor cells Phototoxicity Apoptosis p-53 |
description |
Background: Photodynamic therapy (PDT) has been established in several countries as an alternative therapy for the treatment of various malignancies. This therapy involves the incorporation of a photosensitizer (PS) that is activated by visible light and form reactive oxygen species leading to target cell death by apoptosis or necrosis. Previously, our group has demonstrated that CHL-T (semi-synthesized from chlorophyll a and containing a linked solubilizing group TRISMA (R)) presented a pronounced potential to induce death in HeLa cell line after PDT. In the present study, besides confirm the high cytotoxicity in another cell line, we have further investigated the cell death mechanisms caused by CHL-T as a photosensitizer in laryngeal carcinoma cells. Methods: Cells were exposed to different concentrations of three photosensitizers, namely, hypericin (HY), unmodified chlorin (CHL) and a synthesized amphiphilic chlorin derivative (CHL-T). PSs accumulation and localization were accessed by fluorescence assays. Photosensitization was induced at 6 J cm(-2) using red LEDs (630 +/- 10 nm). Viability was assessed by mitochondrial function (MTT); whereas apoptosis/necrosis was evaluated by fluorescence microscopy and flow cytometry. Expression of pro-apoptotic p53 protein was studied by Western blot. Results and conclusions: All PS showed similar localization profile in the HEp-2 cells. The use of CHL-T increased the percentage of apoptotic cells and also p53 expression in comparison with the use of HY and CHL as photosensitizers. This study shows a significant effect of CHLT associated with red light (630 +/- 10 nm and 18 mW cm(-2)) irradiation on a cancer cell line, indicating the potential of this amphiphilic chlorin in enhancing the therapeutic effectiveness of Photodynamic Therapy (PDT). |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 2018-11-26T17:41:42Z 2018-11-26T17:41:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2017.07.003 Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 19, p. 355-362, 2017. 1572-1000 http://hdl.handle.net/11449/163366 10.1016/j.pdpdt.2017.07.003 WOS:000412252100053 WOS000412252100053.pdf |
url |
http://dx.doi.org/10.1016/j.pdpdt.2017.07.003 http://hdl.handle.net/11449/163366 |
identifier_str_mv |
Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 19, p. 355-362, 2017. 1572-1000 10.1016/j.pdpdt.2017.07.003 WOS:000412252100053 WOS000412252100053.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis And Photodynamic Therapy 0,647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
355-362 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128819516669952 |