Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone

Detalhes bibliográficos
Autor(a) principal: Fallah, Hamideh P.
Data de Publicação: 2019
Outros Autores: Tovo-Neto, Aldo [UNESP], Yeung, Edward C., Nóbrega, Rafael H. [UNESP], Habibi, Hamid R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mce.2019.04.020
http://hdl.handle.net/11449/189059
Resumo: Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response.
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spelling Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormoneFlutamide (FLU)Gonadotropin-inhibitory hormone (Gnih)Human chorionic gonadotropin (hCG)Spermatids (SPD)Spermatozoa (SPZ)TestosteroneZebrafish gnih (zGnih)Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response.Natural Sciences and Engineering Research Council of CanadaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biological Sciences University of Calgary, 2500 University Drive NWAquaculture Program São Paulo State University (Unesp), JaboticabalDepartment of Morphology Reproductive and Molecular Biology Group São Paulo State University, BotucatuAquaculture Program São Paulo State University (Unesp), JaboticabalDepartment of Morphology Reproductive and Molecular Biology Group São Paulo State University, BotucatuNatural Sciences and Engineering Research Council of Canada: 1021837FAPESP: 2014/07620-7FAPESP: 2014/16280-5FAPESP: 2017/09218-0University of CalgaryUniversidade Estadual Paulista (Unesp)Fallah, Hamideh P.Tovo-Neto, Aldo [UNESP]Yeung, Edward C.Nóbrega, Rafael H. [UNESP]Habibi, Hamid R.2019-10-06T16:28:27Z2019-10-06T16:28:27Z2019-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.mce.2019.04.020Molecular and Cellular Endocrinology, v. 492.1872-80570303-7207http://hdl.handle.net/11449/18905910.1016/j.mce.2019.04.0202-s2.0-8506507103405157085852539850000-0001-9796-5076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Endocrinologyinfo:eu-repo/semantics/openAccess2024-04-09T15:10:46Zoai:repositorio.unesp.br:11449/189059Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:15:24.804957Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
title Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
spellingShingle Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
Fallah, Hamideh P.
Flutamide (FLU)
Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Spermatids (SPD)
Spermatozoa (SPZ)
Testosterone
Zebrafish gnih (zGnih)
title_short Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
title_full Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
title_fullStr Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
title_full_unstemmed Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
title_sort Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
author Fallah, Hamideh P.
author_facet Fallah, Hamideh P.
Tovo-Neto, Aldo [UNESP]
Yeung, Edward C.
Nóbrega, Rafael H. [UNESP]
Habibi, Hamid R.
author_role author
author2 Tovo-Neto, Aldo [UNESP]
Yeung, Edward C.
Nóbrega, Rafael H. [UNESP]
Habibi, Hamid R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv University of Calgary
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fallah, Hamideh P.
Tovo-Neto, Aldo [UNESP]
Yeung, Edward C.
Nóbrega, Rafael H. [UNESP]
Habibi, Hamid R.
dc.subject.por.fl_str_mv Flutamide (FLU)
Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Spermatids (SPD)
Spermatozoa (SPZ)
Testosterone
Zebrafish gnih (zGnih)
topic Flutamide (FLU)
Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Spermatids (SPD)
Spermatozoa (SPZ)
Testosterone
Zebrafish gnih (zGnih)
description Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:28:27Z
2019-10-06T16:28:27Z
2019-07-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mce.2019.04.020
Molecular and Cellular Endocrinology, v. 492.
1872-8057
0303-7207
http://hdl.handle.net/11449/189059
10.1016/j.mce.2019.04.020
2-s2.0-85065071034
0515708585253985
0000-0001-9796-5076
url http://dx.doi.org/10.1016/j.mce.2019.04.020
http://hdl.handle.net/11449/189059
identifier_str_mv Molecular and Cellular Endocrinology, v. 492.
1872-8057
0303-7207
10.1016/j.mce.2019.04.020
2-s2.0-85065071034
0515708585253985
0000-0001-9796-5076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular and Cellular Endocrinology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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