Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mce.2019.04.020 http://hdl.handle.net/11449/189059 |
Resumo: | Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response. |
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Repositório Institucional da UNESP |
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Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormoneFlutamide (FLU)Gonadotropin-inhibitory hormone (Gnih)Human chorionic gonadotropin (hCG)Spermatids (SPD)Spermatozoa (SPZ)TestosteroneZebrafish gnih (zGnih)Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response.Natural Sciences and Engineering Research Council of CanadaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biological Sciences University of Calgary, 2500 University Drive NWAquaculture Program São Paulo State University (Unesp), JaboticabalDepartment of Morphology Reproductive and Molecular Biology Group São Paulo State University, BotucatuAquaculture Program São Paulo State University (Unesp), JaboticabalDepartment of Morphology Reproductive and Molecular Biology Group São Paulo State University, BotucatuNatural Sciences and Engineering Research Council of Canada: 1021837FAPESP: 2014/07620-7FAPESP: 2014/16280-5FAPESP: 2017/09218-0University of CalgaryUniversidade Estadual Paulista (Unesp)Fallah, Hamideh P.Tovo-Neto, Aldo [UNESP]Yeung, Edward C.Nóbrega, Rafael H. [UNESP]Habibi, Hamid R.2019-10-06T16:28:27Z2019-10-06T16:28:27Z2019-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.mce.2019.04.020Molecular and Cellular Endocrinology, v. 492.1872-80570303-7207http://hdl.handle.net/11449/18905910.1016/j.mce.2019.04.0202-s2.0-8506507103405157085852539850000-0001-9796-5076Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Endocrinologyinfo:eu-repo/semantics/openAccess2024-04-09T15:10:46Zoai:repositorio.unesp.br:11449/189059Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:15:24.804957Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
title |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
spellingShingle |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone Fallah, Hamideh P. Flutamide (FLU) Gonadotropin-inhibitory hormone (Gnih) Human chorionic gonadotropin (hCG) Spermatids (SPD) Spermatozoa (SPZ) Testosterone Zebrafish gnih (zGnih) |
title_short |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
title_full |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
title_fullStr |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
title_full_unstemmed |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
title_sort |
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone |
author |
Fallah, Hamideh P. |
author_facet |
Fallah, Hamideh P. Tovo-Neto, Aldo [UNESP] Yeung, Edward C. Nóbrega, Rafael H. [UNESP] Habibi, Hamid R. |
author_role |
author |
author2 |
Tovo-Neto, Aldo [UNESP] Yeung, Edward C. Nóbrega, Rafael H. [UNESP] Habibi, Hamid R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
University of Calgary Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Fallah, Hamideh P. Tovo-Neto, Aldo [UNESP] Yeung, Edward C. Nóbrega, Rafael H. [UNESP] Habibi, Hamid R. |
dc.subject.por.fl_str_mv |
Flutamide (FLU) Gonadotropin-inhibitory hormone (Gnih) Human chorionic gonadotropin (hCG) Spermatids (SPD) Spermatozoa (SPZ) Testosterone Zebrafish gnih (zGnih) |
topic |
Flutamide (FLU) Gonadotropin-inhibitory hormone (Gnih) Human chorionic gonadotropin (hCG) Spermatids (SPD) Spermatozoa (SPZ) Testosterone Zebrafish gnih (zGnih) |
description |
Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:28:27Z 2019-10-06T16:28:27Z 2019-07-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mce.2019.04.020 Molecular and Cellular Endocrinology, v. 492. 1872-8057 0303-7207 http://hdl.handle.net/11449/189059 10.1016/j.mce.2019.04.020 2-s2.0-85065071034 0515708585253985 0000-0001-9796-5076 |
url |
http://dx.doi.org/10.1016/j.mce.2019.04.020 http://hdl.handle.net/11449/189059 |
identifier_str_mv |
Molecular and Cellular Endocrinology, v. 492. 1872-8057 0303-7207 10.1016/j.mce.2019.04.020 2-s2.0-85065071034 0515708585253985 0000-0001-9796-5076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular and Cellular Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128489487859712 |