Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mce.2020.111087 http://hdl.handle.net/11449/209808 |
Resumo: | Gonadotropin-inhibitory hormone (Gnih) is known to play a role in the regulation of reproduction in vertebrates by influencing gonadotmpin release and synthesis. While the endocrine actions of Gnih have been identified in several species, its paracrine/autocrine effects in the control of spermatogenesis are less defined. We have used ex vivo culture of zebrafish testis to investigate the role of gonadal zebrafish Gnih (zGnih) in the regulation of the spermatogenic process. We used FACScan cell cycle analysis, morphometric quantifications, BrdU incorporation and caspase-3 activity assays as well as measuring 11-Ketotestosterone (11-KT) level in the culture media. FACScan analysis and morphometric quantification results demonstrated direct action of zGnih on basal and gonadotropin (Lh and Fsh)-induced spermatogenesis. Treatment with zGnih (10 nM) significantly decreased the number of G0/G1 cells after 7-days of culture while no significant changes were found in the proportion area of spermatogonia cell types. Investigation of DNA synthesis using BrdU (5-Bromo-2'-Deoxyuridine) labeling showed that treatment with zGnih (10 nM) significantly decreased proliferative activity of type A spermatogonia, while increased the mitotic activity of type B spermatogonia. We also showed that treatment with zGnih (100 nM) completely eliminated 11-KT release induced by 100 ng/ml Fsh. Treatment with zGnih (10 and 100 nM) also inhibited both hCG and Fsh-induced spermatogenesis. These results, plus our previous findings, demonstrate that zGnih produced locally in the testis is a component of a complex multifactorial system that regulates testicular function in zebrafish. |
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Repositório Institucional da UNESP |
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Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testisGonadotropin-inhibitory hormone (Gnih)Human chorionic gonadotropin (hCG)Follicle-stimulating hormone (Fsh)Gonadotropin-inhibitory hormone (Gnih) is known to play a role in the regulation of reproduction in vertebrates by influencing gonadotmpin release and synthesis. While the endocrine actions of Gnih have been identified in several species, its paracrine/autocrine effects in the control of spermatogenesis are less defined. We have used ex vivo culture of zebrafish testis to investigate the role of gonadal zebrafish Gnih (zGnih) in the regulation of the spermatogenic process. We used FACScan cell cycle analysis, morphometric quantifications, BrdU incorporation and caspase-3 activity assays as well as measuring 11-Ketotestosterone (11-KT) level in the culture media. FACScan analysis and morphometric quantification results demonstrated direct action of zGnih on basal and gonadotropin (Lh and Fsh)-induced spermatogenesis. Treatment with zGnih (10 nM) significantly decreased the number of G0/G1 cells after 7-days of culture while no significant changes were found in the proportion area of spermatogonia cell types. Investigation of DNA synthesis using BrdU (5-Bromo-2'-Deoxyuridine) labeling showed that treatment with zGnih (10 nM) significantly decreased proliferative activity of type A spermatogonia, while increased the mitotic activity of type B spermatogonia. We also showed that treatment with zGnih (100 nM) completely eliminated 11-KT release induced by 100 ng/ml Fsh. Treatment with zGnih (10 and 100 nM) also inhibited both hCG and Fsh-induced spermatogenesis. These results, plus our previous findings, demonstrate that zGnih produced locally in the testis is a component of a complex multifactorial system that regulates testicular function in zebrafish.Natural Sciences and Engineering Research Council (NSERC) of CanadaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Calgary, Dept Biol Sci, 2500 Univ Dr NW, Calgary, AB T2N 1N4, CanadaSao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilSao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilNatural Sciences and Engineering Research Council (NSERC) of Canada: 1021837FAPESP: 17/15793-7FAPESP: 18/15319-6Elsevier B.V.Univ CalgaryUniversidade Estadual Paulista (Unesp)Fallah, Hamideh P.Rodrigues, Maira S. [UNESP]Zanardini, MayaNobrega, Rafael H. [UNESP]Habibi, Hamid R.2021-06-25T12:29:54Z2021-06-25T12:29:54Z2021-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.mce.2020.111087Molecular And Cellular Endocrinology. Clare: Elsevier Ireland Ltd, v. 520, 10 p., 2021.0303-7207http://hdl.handle.net/11449/20980810.1016/j.mce.2020.111087WOS:000600756700020Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular And Cellular Endocrinologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:45:52.471337Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
title |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
spellingShingle |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis Fallah, Hamideh P. Gonadotropin-inhibitory hormone (Gnih) Human chorionic gonadotropin (hCG) Follicle-stimulating hormone (Fsh) |
title_short |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
title_full |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
title_fullStr |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
title_full_unstemmed |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
title_sort |
Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis |
author |
Fallah, Hamideh P. |
author_facet |
Fallah, Hamideh P. Rodrigues, Maira S. [UNESP] Zanardini, Maya Nobrega, Rafael H. [UNESP] Habibi, Hamid R. |
author_role |
author |
author2 |
Rodrigues, Maira S. [UNESP] Zanardini, Maya Nobrega, Rafael H. [UNESP] Habibi, Hamid R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Calgary Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Fallah, Hamideh P. Rodrigues, Maira S. [UNESP] Zanardini, Maya Nobrega, Rafael H. [UNESP] Habibi, Hamid R. |
dc.subject.por.fl_str_mv |
Gonadotropin-inhibitory hormone (Gnih) Human chorionic gonadotropin (hCG) Follicle-stimulating hormone (Fsh) |
topic |
Gonadotropin-inhibitory hormone (Gnih) Human chorionic gonadotropin (hCG) Follicle-stimulating hormone (Fsh) |
description |
Gonadotropin-inhibitory hormone (Gnih) is known to play a role in the regulation of reproduction in vertebrates by influencing gonadotmpin release and synthesis. While the endocrine actions of Gnih have been identified in several species, its paracrine/autocrine effects in the control of spermatogenesis are less defined. We have used ex vivo culture of zebrafish testis to investigate the role of gonadal zebrafish Gnih (zGnih) in the regulation of the spermatogenic process. We used FACScan cell cycle analysis, morphometric quantifications, BrdU incorporation and caspase-3 activity assays as well as measuring 11-Ketotestosterone (11-KT) level in the culture media. FACScan analysis and morphometric quantification results demonstrated direct action of zGnih on basal and gonadotropin (Lh and Fsh)-induced spermatogenesis. Treatment with zGnih (10 nM) significantly decreased the number of G0/G1 cells after 7-days of culture while no significant changes were found in the proportion area of spermatogonia cell types. Investigation of DNA synthesis using BrdU (5-Bromo-2'-Deoxyuridine) labeling showed that treatment with zGnih (10 nM) significantly decreased proliferative activity of type A spermatogonia, while increased the mitotic activity of type B spermatogonia. We also showed that treatment with zGnih (100 nM) completely eliminated 11-KT release induced by 100 ng/ml Fsh. Treatment with zGnih (10 and 100 nM) also inhibited both hCG and Fsh-induced spermatogenesis. These results, plus our previous findings, demonstrate that zGnih produced locally in the testis is a component of a complex multifactorial system that regulates testicular function in zebrafish. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:29:54Z 2021-06-25T12:29:54Z 2021-01-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mce.2020.111087 Molecular And Cellular Endocrinology. Clare: Elsevier Ireland Ltd, v. 520, 10 p., 2021. 0303-7207 http://hdl.handle.net/11449/209808 10.1016/j.mce.2020.111087 WOS:000600756700020 |
url |
http://dx.doi.org/10.1016/j.mce.2020.111087 http://hdl.handle.net/11449/209808 |
identifier_str_mv |
Molecular And Cellular Endocrinology. Clare: Elsevier Ireland Ltd, v. 520, 10 p., 2021. 0303-7207 10.1016/j.mce.2020.111087 WOS:000600756700020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular And Cellular Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129244354576384 |