Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis

Detalhes bibliográficos
Autor(a) principal: Fallah, Hamideh P.
Data de Publicação: 2021
Outros Autores: Rodrigues, Maira S. [UNESP], Zanardini, Maya, Nobrega, Rafael H. [UNESP], Habibi, Hamid R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mce.2020.111087
http://hdl.handle.net/11449/209808
Resumo: Gonadotropin-inhibitory hormone (Gnih) is known to play a role in the regulation of reproduction in vertebrates by influencing gonadotmpin release and synthesis. While the endocrine actions of Gnih have been identified in several species, its paracrine/autocrine effects in the control of spermatogenesis are less defined. We have used ex vivo culture of zebrafish testis to investigate the role of gonadal zebrafish Gnih (zGnih) in the regulation of the spermatogenic process. We used FACScan cell cycle analysis, morphometric quantifications, BrdU incorporation and caspase-3 activity assays as well as measuring 11-Ketotestosterone (11-KT) level in the culture media. FACScan analysis and morphometric quantification results demonstrated direct action of zGnih on basal and gonadotropin (Lh and Fsh)-induced spermatogenesis. Treatment with zGnih (10 nM) significantly decreased the number of G0/G1 cells after 7-days of culture while no significant changes were found in the proportion area of spermatogonia cell types. Investigation of DNA synthesis using BrdU (5-Bromo-2'-Deoxyuridine) labeling showed that treatment with zGnih (10 nM) significantly decreased proliferative activity of type A spermatogonia, while increased the mitotic activity of type B spermatogonia. We also showed that treatment with zGnih (100 nM) completely eliminated 11-KT release induced by 100 ng/ml Fsh. Treatment with zGnih (10 and 100 nM) also inhibited both hCG and Fsh-induced spermatogenesis. These results, plus our previous findings, demonstrate that zGnih produced locally in the testis is a component of a complex multifactorial system that regulates testicular function in zebrafish.
id UNSP_68b9378c8c31a11ceb81bb098ffad047
oai_identifier_str oai:repositorio.unesp.br:11449/209808
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testisGonadotropin-inhibitory hormone (Gnih)Human chorionic gonadotropin (hCG)Follicle-stimulating hormone (Fsh)Gonadotropin-inhibitory hormone (Gnih) is known to play a role in the regulation of reproduction in vertebrates by influencing gonadotmpin release and synthesis. While the endocrine actions of Gnih have been identified in several species, its paracrine/autocrine effects in the control of spermatogenesis are less defined. We have used ex vivo culture of zebrafish testis to investigate the role of gonadal zebrafish Gnih (zGnih) in the regulation of the spermatogenic process. We used FACScan cell cycle analysis, morphometric quantifications, BrdU incorporation and caspase-3 activity assays as well as measuring 11-Ketotestosterone (11-KT) level in the culture media. FACScan analysis and morphometric quantification results demonstrated direct action of zGnih on basal and gonadotropin (Lh and Fsh)-induced spermatogenesis. Treatment with zGnih (10 nM) significantly decreased the number of G0/G1 cells after 7-days of culture while no significant changes were found in the proportion area of spermatogonia cell types. Investigation of DNA synthesis using BrdU (5-Bromo-2'-Deoxyuridine) labeling showed that treatment with zGnih (10 nM) significantly decreased proliferative activity of type A spermatogonia, while increased the mitotic activity of type B spermatogonia. We also showed that treatment with zGnih (100 nM) completely eliminated 11-KT release induced by 100 ng/ml Fsh. Treatment with zGnih (10 and 100 nM) also inhibited both hCG and Fsh-induced spermatogenesis. These results, plus our previous findings, demonstrate that zGnih produced locally in the testis is a component of a complex multifactorial system that regulates testicular function in zebrafish.Natural Sciences and Engineering Research Council (NSERC) of CanadaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Calgary, Dept Biol Sci, 2500 Univ Dr NW, Calgary, AB T2N 1N4, CanadaSao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilSao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilNatural Sciences and Engineering Research Council (NSERC) of Canada: 1021837FAPESP: 17/15793-7FAPESP: 18/15319-6Elsevier B.V.Univ CalgaryUniversidade Estadual Paulista (Unesp)Fallah, Hamideh P.Rodrigues, Maira S. [UNESP]Zanardini, MayaNobrega, Rafael H. [UNESP]Habibi, Hamid R.2021-06-25T12:29:54Z2021-06-25T12:29:54Z2021-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.mce.2020.111087Molecular And Cellular Endocrinology. Clare: Elsevier Ireland Ltd, v. 520, 10 p., 2021.0303-7207http://hdl.handle.net/11449/20980810.1016/j.mce.2020.111087WOS:000600756700020Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular And Cellular Endocrinologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:45:52.471337Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
title Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
spellingShingle Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
Fallah, Hamideh P.
Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Follicle-stimulating hormone (Fsh)
title_short Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
title_full Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
title_fullStr Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
title_full_unstemmed Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
title_sort Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis
author Fallah, Hamideh P.
author_facet Fallah, Hamideh P.
Rodrigues, Maira S. [UNESP]
Zanardini, Maya
Nobrega, Rafael H. [UNESP]
Habibi, Hamid R.
author_role author
author2 Rodrigues, Maira S. [UNESP]
Zanardini, Maya
Nobrega, Rafael H. [UNESP]
Habibi, Hamid R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Univ Calgary
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fallah, Hamideh P.
Rodrigues, Maira S. [UNESP]
Zanardini, Maya
Nobrega, Rafael H. [UNESP]
Habibi, Hamid R.
dc.subject.por.fl_str_mv Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Follicle-stimulating hormone (Fsh)
topic Gonadotropin-inhibitory hormone (Gnih)
Human chorionic gonadotropin (hCG)
Follicle-stimulating hormone (Fsh)
description Gonadotropin-inhibitory hormone (Gnih) is known to play a role in the regulation of reproduction in vertebrates by influencing gonadotmpin release and synthesis. While the endocrine actions of Gnih have been identified in several species, its paracrine/autocrine effects in the control of spermatogenesis are less defined. We have used ex vivo culture of zebrafish testis to investigate the role of gonadal zebrafish Gnih (zGnih) in the regulation of the spermatogenic process. We used FACScan cell cycle analysis, morphometric quantifications, BrdU incorporation and caspase-3 activity assays as well as measuring 11-Ketotestosterone (11-KT) level in the culture media. FACScan analysis and morphometric quantification results demonstrated direct action of zGnih on basal and gonadotropin (Lh and Fsh)-induced spermatogenesis. Treatment with zGnih (10 nM) significantly decreased the number of G0/G1 cells after 7-days of culture while no significant changes were found in the proportion area of spermatogonia cell types. Investigation of DNA synthesis using BrdU (5-Bromo-2'-Deoxyuridine) labeling showed that treatment with zGnih (10 nM) significantly decreased proliferative activity of type A spermatogonia, while increased the mitotic activity of type B spermatogonia. We also showed that treatment with zGnih (100 nM) completely eliminated 11-KT release induced by 100 ng/ml Fsh. Treatment with zGnih (10 and 100 nM) also inhibited both hCG and Fsh-induced spermatogenesis. These results, plus our previous findings, demonstrate that zGnih produced locally in the testis is a component of a complex multifactorial system that regulates testicular function in zebrafish.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:29:54Z
2021-06-25T12:29:54Z
2021-01-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mce.2020.111087
Molecular And Cellular Endocrinology. Clare: Elsevier Ireland Ltd, v. 520, 10 p., 2021.
0303-7207
http://hdl.handle.net/11449/209808
10.1016/j.mce.2020.111087
WOS:000600756700020
url http://dx.doi.org/10.1016/j.mce.2020.111087
http://hdl.handle.net/11449/209808
identifier_str_mv Molecular And Cellular Endocrinology. Clare: Elsevier Ireland Ltd, v. 520, 10 p., 2021.
0303-7207
10.1016/j.mce.2020.111087
WOS:000600756700020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular And Cellular Endocrinology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129244354576384