MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis

Detalhes bibliográficos
Autor(a) principal: De Freitas, Jéssica Henrique [UNESP]
Data de Publicação: 2023
Outros Autores: Bragato, Jaqueline Poleto [UNESP], Rebech, Gabriela Torres [UNESP], Costa, Sidnei Ferro [UNESP], Dos Santos, Marilene Oliveira [UNESP], Soares, Matheus Fujimura [UNESP], Eugênio, Flávia de Rezende [UNESP], Dos Santos, Paulo Sérgio Patto [UNESP], De Lima, Valéria Marçal Felix [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fgene.2023.1106496
http://hdl.handle.net/11449/249899
Resumo: Canine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels.
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spelling MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasisLeishmania infantummicroRNAnitriteNOptenROSsplenic leukocytesCanine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)Department of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)FAPESP: 2018/17261-5 2021/07283-4Universidade Estadual Paulista (UNESP)De Freitas, Jéssica Henrique [UNESP]Bragato, Jaqueline Poleto [UNESP]Rebech, Gabriela Torres [UNESP]Costa, Sidnei Ferro [UNESP]Dos Santos, Marilene Oliveira [UNESP]Soares, Matheus Fujimura [UNESP]Eugênio, Flávia de Rezende [UNESP]Dos Santos, Paulo Sérgio Patto [UNESP]De Lima, Valéria Marçal Felix [UNESP]2023-07-29T16:12:19Z2023-07-29T16:12:19Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fgene.2023.1106496Frontiers in Genetics, v. 14.1664-8021http://hdl.handle.net/11449/24989910.3389/fgene.2023.11064962-s2.0-85153709256Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Geneticsinfo:eu-repo/semantics/openAccess2024-09-04T18:03:43Zoai:repositorio.unesp.br:11449/249899Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
title MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
spellingShingle MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
De Freitas, Jéssica Henrique [UNESP]
Leishmania infantum
microRNA
nitrite
NO
pten
ROS
splenic leukocytes
title_short MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
title_full MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
title_fullStr MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
title_full_unstemmed MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
title_sort MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
author De Freitas, Jéssica Henrique [UNESP]
author_facet De Freitas, Jéssica Henrique [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Rebech, Gabriela Torres [UNESP]
Costa, Sidnei Ferro [UNESP]
Dos Santos, Marilene Oliveira [UNESP]
Soares, Matheus Fujimura [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Dos Santos, Paulo Sérgio Patto [UNESP]
De Lima, Valéria Marçal Felix [UNESP]
author_role author
author2 Bragato, Jaqueline Poleto [UNESP]
Rebech, Gabriela Torres [UNESP]
Costa, Sidnei Ferro [UNESP]
Dos Santos, Marilene Oliveira [UNESP]
Soares, Matheus Fujimura [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Dos Santos, Paulo Sérgio Patto [UNESP]
De Lima, Valéria Marçal Felix [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv De Freitas, Jéssica Henrique [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Rebech, Gabriela Torres [UNESP]
Costa, Sidnei Ferro [UNESP]
Dos Santos, Marilene Oliveira [UNESP]
Soares, Matheus Fujimura [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Dos Santos, Paulo Sérgio Patto [UNESP]
De Lima, Valéria Marçal Felix [UNESP]
dc.subject.por.fl_str_mv Leishmania infantum
microRNA
nitrite
NO
pten
ROS
splenic leukocytes
topic Leishmania infantum
microRNA
nitrite
NO
pten
ROS
splenic leukocytes
description Canine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:12:19Z
2023-07-29T16:12:19Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fgene.2023.1106496
Frontiers in Genetics, v. 14.
1664-8021
http://hdl.handle.net/11449/249899
10.3389/fgene.2023.1106496
2-s2.0-85153709256
url http://dx.doi.org/10.3389/fgene.2023.1106496
http://hdl.handle.net/11449/249899
identifier_str_mv Frontiers in Genetics, v. 14.
1664-8021
10.3389/fgene.2023.1106496
2-s2.0-85153709256
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Genetics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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