MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fgene.2023.1106496 http://hdl.handle.net/11449/249899 |
Resumo: | Canine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels. |
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MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasisLeishmania infantummicroRNAnitriteNOptenROSsplenic leukocytesCanine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)Department of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)FAPESP: 2018/17261-5 2021/07283-4Universidade Estadual Paulista (UNESP)De Freitas, Jéssica Henrique [UNESP]Bragato, Jaqueline Poleto [UNESP]Rebech, Gabriela Torres [UNESP]Costa, Sidnei Ferro [UNESP]Dos Santos, Marilene Oliveira [UNESP]Soares, Matheus Fujimura [UNESP]Eugênio, Flávia de Rezende [UNESP]Dos Santos, Paulo Sérgio Patto [UNESP]De Lima, Valéria Marçal Felix [UNESP]2023-07-29T16:12:19Z2023-07-29T16:12:19Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fgene.2023.1106496Frontiers in Genetics, v. 14.1664-8021http://hdl.handle.net/11449/24989910.3389/fgene.2023.11064962-s2.0-85153709256Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Geneticsinfo:eu-repo/semantics/openAccess2024-09-04T18:03:43Zoai:repositorio.unesp.br:11449/249899Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
title |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
spellingShingle |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis De Freitas, Jéssica Henrique [UNESP] Leishmania infantum microRNA nitrite NO pten ROS splenic leukocytes |
title_short |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
title_full |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
title_fullStr |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
title_full_unstemmed |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
title_sort |
MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis |
author |
De Freitas, Jéssica Henrique [UNESP] |
author_facet |
De Freitas, Jéssica Henrique [UNESP] Bragato, Jaqueline Poleto [UNESP] Rebech, Gabriela Torres [UNESP] Costa, Sidnei Ferro [UNESP] Dos Santos, Marilene Oliveira [UNESP] Soares, Matheus Fujimura [UNESP] Eugênio, Flávia de Rezende [UNESP] Dos Santos, Paulo Sérgio Patto [UNESP] De Lima, Valéria Marçal Felix [UNESP] |
author_role |
author |
author2 |
Bragato, Jaqueline Poleto [UNESP] Rebech, Gabriela Torres [UNESP] Costa, Sidnei Ferro [UNESP] Dos Santos, Marilene Oliveira [UNESP] Soares, Matheus Fujimura [UNESP] Eugênio, Flávia de Rezende [UNESP] Dos Santos, Paulo Sérgio Patto [UNESP] De Lima, Valéria Marçal Felix [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
De Freitas, Jéssica Henrique [UNESP] Bragato, Jaqueline Poleto [UNESP] Rebech, Gabriela Torres [UNESP] Costa, Sidnei Ferro [UNESP] Dos Santos, Marilene Oliveira [UNESP] Soares, Matheus Fujimura [UNESP] Eugênio, Flávia de Rezende [UNESP] Dos Santos, Paulo Sérgio Patto [UNESP] De Lima, Valéria Marçal Felix [UNESP] |
dc.subject.por.fl_str_mv |
Leishmania infantum microRNA nitrite NO pten ROS splenic leukocytes |
topic |
Leishmania infantum microRNA nitrite NO pten ROS splenic leukocytes |
description |
Canine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:12:19Z 2023-07-29T16:12:19Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fgene.2023.1106496 Frontiers in Genetics, v. 14. 1664-8021 http://hdl.handle.net/11449/249899 10.3389/fgene.2023.1106496 2-s2.0-85153709256 |
url |
http://dx.doi.org/10.3389/fgene.2023.1106496 http://hdl.handle.net/11449/249899 |
identifier_str_mv |
Frontiers in Genetics, v. 14. 1664-8021 10.3389/fgene.2023.1106496 2-s2.0-85153709256 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021370033078272 |