Molecular study on endemic cattleya species from brazilian flora
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://aijbnet.com/vol-2-no-3-4-july-december-2014-abstract-5-aijb http://hdl.handle.net/11449/126967 |
Resumo: | The orchids are known for its beautiful flowers, the genetic recombination ability and the lack of its defined taxonomic classification of the Orchidaceae family. Phylogenetic studies using molecular technics allow additional taxonomy understanding. ISSR molecular markers can determine the phylogenetic relationships among species and individuals of the same species. In this study the patterns of polymorphism were compared in subjects of Cattleya guttata, C. tigrina and C. leopoldii in order to find variations in the germplasm which can contribute to elucidate phylogenetic issues. Results were obtained using 13 primers in ten subjects of each species. They increased 97 loci being 96.9% polymorphic for the three studied species. It is concluded that the protocol was effective for the identification of fragments in all studied species. The analysis of total banding pattern suggests that C. guttata and C. leopoldii are molecularly related to each other and more distant from C. tigrina. |
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Molecular study on endemic cattleya species from brazilian floraCattleyaPlantas NativasMarcadores molecularesGermoplasmaThe orchids are known for its beautiful flowers, the genetic recombination ability and the lack of its defined taxonomic classification of the Orchidaceae family. Phylogenetic studies using molecular technics allow additional taxonomy understanding. ISSR molecular markers can determine the phylogenetic relationships among species and individuals of the same species. In this study the patterns of polymorphism were compared in subjects of Cattleya guttata, C. tigrina and C. leopoldii in order to find variations in the germplasm which can contribute to elucidate phylogenetic issues. Results were obtained using 13 primers in ten subjects of each species. They increased 97 loci being 96.9% polymorphic for the three studied species. It is concluded that the protocol was effective for the identification of fragments in all studied species. The analysis of total banding pattern suggests that C. guttata and C. leopoldii are molecularly related to each other and more distant from C. tigrina.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Faculdade de Ciências e Letras de Assis, Assis, Avenida Dom Antonio, 2100, Parque Universitário, CEP 19806900, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Faculdade de Ciências e Letras de Assis, Assis, Avenida Dom Antonio, 2100, Parque Universitário, CEP 19806900, SP, BrasilFAPESP: 2012/16563-1Universidade Estadual Paulista (Unesp)Moraes, Milena Cristina [UNESP]Bertão, Mônica Rosa [UNESP]Loose, Priscila Ventura [UNESP]Cordeiro, Anderson Felipe dos Santos [UNESP]Palmieri, Darío Abel [UNESP]2015-08-21T17:53:36Z2015-08-21T17:53:36Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article77-84http://aijbnet.com/vol-2-no-3-4-july-december-2014-abstract-5-aijbAmerican International Journal of Biology, v. 2, n. 3, p. 77-84, 2014.2334-2331http://hdl.handle.net/11449/12696710.15640/aijb.v2n3-4a58532892625837297447802104358458126274147124228174925484119116186Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAmerican International Journal of Biologyinfo:eu-repo/semantics/openAccess2024-06-13T17:38:53Zoai:repositorio.unesp.br:11449/126967Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:54:20.212698Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular study on endemic cattleya species from brazilian flora |
title |
Molecular study on endemic cattleya species from brazilian flora |
spellingShingle |
Molecular study on endemic cattleya species from brazilian flora Moraes, Milena Cristina [UNESP] Cattleya Plantas Nativas Marcadores moleculares Germoplasma |
title_short |
Molecular study on endemic cattleya species from brazilian flora |
title_full |
Molecular study on endemic cattleya species from brazilian flora |
title_fullStr |
Molecular study on endemic cattleya species from brazilian flora |
title_full_unstemmed |
Molecular study on endemic cattleya species from brazilian flora |
title_sort |
Molecular study on endemic cattleya species from brazilian flora |
author |
Moraes, Milena Cristina [UNESP] |
author_facet |
Moraes, Milena Cristina [UNESP] Bertão, Mônica Rosa [UNESP] Loose, Priscila Ventura [UNESP] Cordeiro, Anderson Felipe dos Santos [UNESP] Palmieri, Darío Abel [UNESP] |
author_role |
author |
author2 |
Bertão, Mônica Rosa [UNESP] Loose, Priscila Ventura [UNESP] Cordeiro, Anderson Felipe dos Santos [UNESP] Palmieri, Darío Abel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moraes, Milena Cristina [UNESP] Bertão, Mônica Rosa [UNESP] Loose, Priscila Ventura [UNESP] Cordeiro, Anderson Felipe dos Santos [UNESP] Palmieri, Darío Abel [UNESP] |
dc.subject.por.fl_str_mv |
Cattleya Plantas Nativas Marcadores moleculares Germoplasma |
topic |
Cattleya Plantas Nativas Marcadores moleculares Germoplasma |
description |
The orchids are known for its beautiful flowers, the genetic recombination ability and the lack of its defined taxonomic classification of the Orchidaceae family. Phylogenetic studies using molecular technics allow additional taxonomy understanding. ISSR molecular markers can determine the phylogenetic relationships among species and individuals of the same species. In this study the patterns of polymorphism were compared in subjects of Cattleya guttata, C. tigrina and C. leopoldii in order to find variations in the germplasm which can contribute to elucidate phylogenetic issues. Results were obtained using 13 primers in ten subjects of each species. They increased 97 loci being 96.9% polymorphic for the three studied species. It is concluded that the protocol was effective for the identification of fragments in all studied species. The analysis of total banding pattern suggests that C. guttata and C. leopoldii are molecularly related to each other and more distant from C. tigrina. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-08-21T17:53:36Z 2015-08-21T17:53:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://aijbnet.com/vol-2-no-3-4-july-december-2014-abstract-5-aijb American International Journal of Biology, v. 2, n. 3, p. 77-84, 2014. 2334-2331 http://hdl.handle.net/11449/126967 10.15640/aijb.v2n3-4a5 8532892625837297 4478021043584581 2627414712422817 4925484119116186 |
url |
http://aijbnet.com/vol-2-no-3-4-july-december-2014-abstract-5-aijb http://hdl.handle.net/11449/126967 |
identifier_str_mv |
American International Journal of Biology, v. 2, n. 3, p. 77-84, 2014. 2334-2331 10.15640/aijb.v2n3-4a5 8532892625837297 4478021043584581 2627414712422817 4925484119116186 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
American International Journal of Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
77-84 |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129371965227008 |