Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil

Detalhes bibliográficos
Autor(a) principal: Noguti, Erika Noda
Data de Publicação: 2010
Outros Autores: Leite, Clarice Queico Fujimura [UNESP], Malaspina, Ana Carolina [UNESP], Santos, Adolfo Carlos Barreto [UNESP], Hirata, Rosário Dominguez Crespo, Hirata, Mario Hiroyuki, Mamizuka, Elsa Massae, Cardoso, Rosilene Fressatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762010000600008
http://hdl.handle.net/11449/7947
Resumo: The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating.
id UNSP_86250560116c7b3c1cd18c235895bf08
oai_identifier_str oai:repositorio.unesp.br:11449/7947
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern BrazilMycobacterium tuberculosistuberculosismolecular epidemiologyspoligotypingMIRUThe purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating.Universidade Estadual de Maringá (UEM) Departamento de Análises ClínicasUniversidade Estadual de São Paulo Faculdade de Ciências FarmacêuticasUniversidade de São Paulo Faculdade de Ciências FarmacêuticasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Noguti, Erika NodaLeite, Clarice Queico Fujimura [UNESP]Malaspina, Ana Carolina [UNESP]Santos, Adolfo Carlos Barreto [UNESP]Hirata, Rosário Dominguez CrespoHirata, Mario HiroyukiMamizuka, Elsa MassaeCardoso, Rosilene Fressatti2014-05-20T13:25:07Z2014-05-20T13:25:07Z2010-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article779-785application/pdfhttp://dx.doi.org/10.1590/S0074-02762010000600008Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 6, p. 779-785, 2010.0074-0276http://hdl.handle.net/11449/794710.1590/S0074-02762010000600008S0074-02762010000600008S0074-02762010000600008.pdf2114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:07:38Zoai:repositorio.unesp.br:11449/7947Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:06:06.261529Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
title Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
spellingShingle Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
Noguti, Erika Noda
Mycobacterium tuberculosis
tuberculosis
molecular epidemiology
spoligotyping
MIRU
title_short Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
title_full Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
title_fullStr Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
title_full_unstemmed Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
title_sort Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
author Noguti, Erika Noda
author_facet Noguti, Erika Noda
Leite, Clarice Queico Fujimura [UNESP]
Malaspina, Ana Carolina [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Hirata, Rosário Dominguez Crespo
Hirata, Mario Hiroyuki
Mamizuka, Elsa Massae
Cardoso, Rosilene Fressatti
author_role author
author2 Leite, Clarice Queico Fujimura [UNESP]
Malaspina, Ana Carolina [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Hirata, Rosário Dominguez Crespo
Hirata, Mario Hiroyuki
Mamizuka, Elsa Massae
Cardoso, Rosilene Fressatti
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Noguti, Erika Noda
Leite, Clarice Queico Fujimura [UNESP]
Malaspina, Ana Carolina [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Hirata, Rosário Dominguez Crespo
Hirata, Mario Hiroyuki
Mamizuka, Elsa Massae
Cardoso, Rosilene Fressatti
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
tuberculosis
molecular epidemiology
spoligotyping
MIRU
topic Mycobacterium tuberculosis
tuberculosis
molecular epidemiology
spoligotyping
MIRU
description The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-01
2014-05-20T13:25:07Z
2014-05-20T13:25:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762010000600008
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 6, p. 779-785, 2010.
0074-0276
http://hdl.handle.net/11449/7947
10.1590/S0074-02762010000600008
S0074-02762010000600008
S0074-02762010000600008.pdf
2114570774349859
url http://dx.doi.org/10.1590/S0074-02762010000600008
http://hdl.handle.net/11449/7947
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 6, p. 779-785, 2010.
0074-0276
10.1590/S0074-02762010000600008
S0074-02762010000600008
S0074-02762010000600008.pdf
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 779-785
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128894142775296