Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762010000600008 http://hdl.handle.net/11449/7947 |
Resumo: | The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating. |
id |
UNSP_86250560116c7b3c1cd18c235895bf08 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/7947 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern BrazilMycobacterium tuberculosistuberculosismolecular epidemiologyspoligotypingMIRUThe purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating.Universidade Estadual de Maringá (UEM) Departamento de Análises ClínicasUniversidade Estadual de São Paulo Faculdade de Ciências FarmacêuticasUniversidade de São Paulo Faculdade de Ciências FarmacêuticasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Noguti, Erika NodaLeite, Clarice Queico Fujimura [UNESP]Malaspina, Ana Carolina [UNESP]Santos, Adolfo Carlos Barreto [UNESP]Hirata, Rosário Dominguez CrespoHirata, Mario HiroyukiMamizuka, Elsa MassaeCardoso, Rosilene Fressatti2014-05-20T13:25:07Z2014-05-20T13:25:07Z2010-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article779-785application/pdfhttp://dx.doi.org/10.1590/S0074-02762010000600008Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 6, p. 779-785, 2010.0074-0276http://hdl.handle.net/11449/794710.1590/S0074-02762010000600008S0074-02762010000600008S0074-02762010000600008.pdf2114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:07:38Zoai:repositorio.unesp.br:11449/7947Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:06:06.261529Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
title |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
spellingShingle |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil Noguti, Erika Noda Mycobacterium tuberculosis tuberculosis molecular epidemiology spoligotyping MIRU |
title_short |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
title_full |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
title_fullStr |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
title_full_unstemmed |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
title_sort |
Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil |
author |
Noguti, Erika Noda |
author_facet |
Noguti, Erika Noda Leite, Clarice Queico Fujimura [UNESP] Malaspina, Ana Carolina [UNESP] Santos, Adolfo Carlos Barreto [UNESP] Hirata, Rosário Dominguez Crespo Hirata, Mario Hiroyuki Mamizuka, Elsa Massae Cardoso, Rosilene Fressatti |
author_role |
author |
author2 |
Leite, Clarice Queico Fujimura [UNESP] Malaspina, Ana Carolina [UNESP] Santos, Adolfo Carlos Barreto [UNESP] Hirata, Rosário Dominguez Crespo Hirata, Mario Hiroyuki Mamizuka, Elsa Massae Cardoso, Rosilene Fressatti |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Noguti, Erika Noda Leite, Clarice Queico Fujimura [UNESP] Malaspina, Ana Carolina [UNESP] Santos, Adolfo Carlos Barreto [UNESP] Hirata, Rosário Dominguez Crespo Hirata, Mario Hiroyuki Mamizuka, Elsa Massae Cardoso, Rosilene Fressatti |
dc.subject.por.fl_str_mv |
Mycobacterium tuberculosis tuberculosis molecular epidemiology spoligotyping MIRU |
topic |
Mycobacterium tuberculosis tuberculosis molecular epidemiology spoligotyping MIRU |
description |
The purpose of this study was to provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis in a low-endemic setting in northwestern state of Paraná in Southern Brazil. We employed spoligotyping and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) techniques to genotype M. tuberculosisisolates from patients with pulmonary tuberculosis (TB). The 93 isolates analyzed by spoligotyping were divided into 36 different patterns, 30 of which were described in the SITVIT database. Latin American and Mediterranean, Haarlem and T families were responsible for 26.9%, 17.2% and 11.8% of TB cases, respectively. From the 84 isolates analyzed by MIRU-VNTR, 58 shared a unique pattern and the remaining 26 belonged to nine clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. A combination of MIRU-VNTR and spoligotyping resulted in 85.7% discriminatory power (Hunter-Gaston index = 0.995). Thus, combining spoligotyping and MIRU-VNTR typing proved to be most useful for epidemiological study in this low-endemic setting in Southern Brazil. The current study demonstrated that there is significant diversity in circulating strains in the city of Maringá and the surrounding regions, with no single genotype of M. tuberculosispredominating. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-01 2014-05-20T13:25:07Z 2014-05-20T13:25:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762010000600008 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 6, p. 779-785, 2010. 0074-0276 http://hdl.handle.net/11449/7947 10.1590/S0074-02762010000600008 S0074-02762010000600008 S0074-02762010000600008.pdf 2114570774349859 |
url |
http://dx.doi.org/10.1590/S0074-02762010000600008 http://hdl.handle.net/11449/7947 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 6, p. 779-785, 2010. 0074-0276 10.1590/S0074-02762010000600008 S0074-02762010000600008 S0074-02762010000600008.pdf 2114570774349859 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
779-785 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128894142775296 |