The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2022.09.010 http://hdl.handle.net/11449/247837 |
Resumo: | Introduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures. |
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The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem CellsApical papilla stem cellscytotoxicitydental pulp stem cellsoctenidineregenerative endodonticsIntroduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Araraquara School of Dentistry São Paulo State University -UNESP, SPDivision of Endodontics University of Minnesota School of DentistryDepartment of Chemistry Ribeirão Preto College of Philosophy Sciences and Letters São Paulo University – USP, SPDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University -UNESP, SPFAPESP: 18/24662-6FAPESP: 20/09576-6Universidade Estadual Paulista (UNESP)School of DentistryUniversidade de São Paulo (USP)Cassiano, Ana Flávia Balestrero [UNESP]Coaguila-Llerena, Hernán [UNESP]Santos, Cíntia Silva [UNESP]da Silva, Luana Raphael [UNESP]Nogueira, Lucas Fabrício BahiaCiancaglini, PietroFaria, Gisele [UNESP]2023-07-29T13:27:12Z2023-07-29T13:27:12Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1502-1510.e1http://dx.doi.org/10.1016/j.joen.2022.09.010Journal of Endodontics, v. 48, n. 12, p. 1502-1510.e1, 2022.0099-2399http://hdl.handle.net/11449/24783710.1016/j.joen.2022.09.0102-s2.0-85141324527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodonticsinfo:eu-repo/semantics/openAccess2024-09-27T18:04:18Zoai:repositorio.unesp.br:11449/247837Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T18:04:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
title |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
spellingShingle |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells Cassiano, Ana Flávia Balestrero [UNESP] Apical papilla stem cells cytotoxicity dental pulp stem cells octenidine regenerative endodontics |
title_short |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
title_full |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
title_fullStr |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
title_full_unstemmed |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
title_sort |
The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells |
author |
Cassiano, Ana Flávia Balestrero [UNESP] |
author_facet |
Cassiano, Ana Flávia Balestrero [UNESP] Coaguila-Llerena, Hernán [UNESP] Santos, Cíntia Silva [UNESP] da Silva, Luana Raphael [UNESP] Nogueira, Lucas Fabrício Bahia Ciancaglini, Pietro Faria, Gisele [UNESP] |
author_role |
author |
author2 |
Coaguila-Llerena, Hernán [UNESP] Santos, Cíntia Silva [UNESP] da Silva, Luana Raphael [UNESP] Nogueira, Lucas Fabrício Bahia Ciancaglini, Pietro Faria, Gisele [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) School of Dentistry Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Cassiano, Ana Flávia Balestrero [UNESP] Coaguila-Llerena, Hernán [UNESP] Santos, Cíntia Silva [UNESP] da Silva, Luana Raphael [UNESP] Nogueira, Lucas Fabrício Bahia Ciancaglini, Pietro Faria, Gisele [UNESP] |
dc.subject.por.fl_str_mv |
Apical papilla stem cells cytotoxicity dental pulp stem cells octenidine regenerative endodontics |
topic |
Apical papilla stem cells cytotoxicity dental pulp stem cells octenidine regenerative endodontics |
description |
Introduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:27:12Z 2023-07-29T13:27:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2022.09.010 Journal of Endodontics, v. 48, n. 12, p. 1502-1510.e1, 2022. 0099-2399 http://hdl.handle.net/11449/247837 10.1016/j.joen.2022.09.010 2-s2.0-85141324527 |
url |
http://dx.doi.org/10.1016/j.joen.2022.09.010 http://hdl.handle.net/11449/247837 |
identifier_str_mv |
Journal of Endodontics, v. 48, n. 12, p. 1502-1510.e1, 2022. 0099-2399 10.1016/j.joen.2022.09.010 2-s2.0-85141324527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Endodontics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1502-1510.e1 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546491138015232 |