The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells

Detalhes bibliográficos
Autor(a) principal: Cassiano, Ana Flávia Balestrero [UNESP]
Data de Publicação: 2022
Outros Autores: Coaguila-Llerena, Hernán [UNESP], Santos, Cíntia Silva [UNESP], da Silva, Luana Raphael [UNESP], Nogueira, Lucas Fabrício Bahia, Ciancaglini, Pietro, Faria, Gisele [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.joen.2022.09.010
http://hdl.handle.net/11449/247837
Resumo: Introduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.
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spelling The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem CellsApical papilla stem cellscytotoxicitydental pulp stem cellsoctenidineregenerative endodonticsIntroduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Araraquara School of Dentistry São Paulo State University -UNESP, SPDivision of Endodontics University of Minnesota School of DentistryDepartment of Chemistry Ribeirão Preto College of Philosophy Sciences and Letters São Paulo University – USP, SPDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University -UNESP, SPFAPESP: 18/24662-6FAPESP: 20/09576-6Universidade Estadual Paulista (UNESP)School of DentistryUniversidade de São Paulo (USP)Cassiano, Ana Flávia Balestrero [UNESP]Coaguila-Llerena, Hernán [UNESP]Santos, Cíntia Silva [UNESP]da Silva, Luana Raphael [UNESP]Nogueira, Lucas Fabrício BahiaCiancaglini, PietroFaria, Gisele [UNESP]2023-07-29T13:27:12Z2023-07-29T13:27:12Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1502-1510.e1http://dx.doi.org/10.1016/j.joen.2022.09.010Journal of Endodontics, v. 48, n. 12, p. 1502-1510.e1, 2022.0099-2399http://hdl.handle.net/11449/24783710.1016/j.joen.2022.09.0102-s2.0-85141324527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodonticsinfo:eu-repo/semantics/openAccess2024-09-27T18:04:18Zoai:repositorio.unesp.br:11449/247837Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T18:04:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
title The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
spellingShingle The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
Cassiano, Ana Flávia Balestrero [UNESP]
Apical papilla stem cells
cytotoxicity
dental pulp stem cells
octenidine
regenerative endodontics
title_short The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
title_full The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
title_fullStr The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
title_full_unstemmed The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
title_sort The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
author Cassiano, Ana Flávia Balestrero [UNESP]
author_facet Cassiano, Ana Flávia Balestrero [UNESP]
Coaguila-Llerena, Hernán [UNESP]
Santos, Cíntia Silva [UNESP]
da Silva, Luana Raphael [UNESP]
Nogueira, Lucas Fabrício Bahia
Ciancaglini, Pietro
Faria, Gisele [UNESP]
author_role author
author2 Coaguila-Llerena, Hernán [UNESP]
Santos, Cíntia Silva [UNESP]
da Silva, Luana Raphael [UNESP]
Nogueira, Lucas Fabrício Bahia
Ciancaglini, Pietro
Faria, Gisele [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
School of Dentistry
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Cassiano, Ana Flávia Balestrero [UNESP]
Coaguila-Llerena, Hernán [UNESP]
Santos, Cíntia Silva [UNESP]
da Silva, Luana Raphael [UNESP]
Nogueira, Lucas Fabrício Bahia
Ciancaglini, Pietro
Faria, Gisele [UNESP]
dc.subject.por.fl_str_mv Apical papilla stem cells
cytotoxicity
dental pulp stem cells
octenidine
regenerative endodontics
topic Apical papilla stem cells
cytotoxicity
dental pulp stem cells
octenidine
regenerative endodontics
description Introduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-01
2023-07-29T13:27:12Z
2023-07-29T13:27:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.joen.2022.09.010
Journal of Endodontics, v. 48, n. 12, p. 1502-1510.e1, 2022.
0099-2399
http://hdl.handle.net/11449/247837
10.1016/j.joen.2022.09.010
2-s2.0-85141324527
url http://dx.doi.org/10.1016/j.joen.2022.09.010
http://hdl.handle.net/11449/247837
identifier_str_mv Journal of Endodontics, v. 48, n. 12, p. 1502-1510.e1, 2022.
0099-2399
10.1016/j.joen.2022.09.010
2-s2.0-85141324527
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Endodontics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1502-1510.e1
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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