Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/srep20377 http://hdl.handle.net/11449/168364 |
Resumo: | The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters. |
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Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposureThe so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters.Laboratory of Ecophysiology and Molecular Evolution National Institute for Amazonian ResearchDepartment of Chemistry and Biochemistry Wilfrid Laurier UniversityDepartment of Biology McMaster UniversityDepartment of Zoology University of British ColumbiaBiosciences Institute Saõ Paulo State University-UNESP Coastal CampusBiosciences Institute Saõ Paulo State University-UNESP Coastal CampusNational Institute for Amazonian ResearchWilfrid Laurier UniversityMcMaster UniversityUniversity of British ColumbiaUniversidade Estadual Paulista (Unesp)Duarte, Rafael M. [UNESP]Smith, D. ScottVal, Adalberto L.Wood, Chris M.2018-12-11T16:40:57Z2018-12-11T16:40:57Z2016-02-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1038/srep20377Scientific Reports, v. 6.2045-2322http://hdl.handle.net/11449/16836410.1038/srep203772-s2.0-849575851992-s2.0-84957585199.pdf30557957777876120000-0001-5649-0692Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-11-09T06:16:02Zoai:repositorio.unesp.br:11449/168364Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:17:04.195482Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
spellingShingle |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure Duarte, Rafael M. [UNESP] |
title_short |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_full |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_fullStr |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_full_unstemmed |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
title_sort |
Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure |
author |
Duarte, Rafael M. [UNESP] |
author_facet |
Duarte, Rafael M. [UNESP] Smith, D. Scott Val, Adalberto L. Wood, Chris M. |
author_role |
author |
author2 |
Smith, D. Scott Val, Adalberto L. Wood, Chris M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
National Institute for Amazonian Research Wilfrid Laurier University McMaster University University of British Columbia Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Duarte, Rafael M. [UNESP] Smith, D. Scott Val, Adalberto L. Wood, Chris M. |
description |
The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-08 2018-12-11T16:40:57Z 2018-12-11T16:40:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/srep20377 Scientific Reports, v. 6. 2045-2322 http://hdl.handle.net/11449/168364 10.1038/srep20377 2-s2.0-84957585199 2-s2.0-84957585199.pdf 3055795777787612 0000-0001-5649-0692 |
url |
http://dx.doi.org/10.1038/srep20377 http://hdl.handle.net/11449/168364 |
identifier_str_mv |
Scientific Reports, v. 6. 2045-2322 10.1038/srep20377 2-s2.0-84957585199 2-s2.0-84957585199.pdf 3055795777787612 0000-0001-5649-0692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128783967846400 |