Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183

Detalhes bibliográficos
Autor(a) principal: Sivieri, Katia [UNESP]
Data de Publicação: 2011
Outros Autores: Bianchi, Fernanda [UNESP], Tallarico, Maria A., Rossi, Elizeu A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.31989/ffhd.v1i10.119
http://hdl.handle.net/11449/227015
Resumo: Background: Enterococci are used in a large number of dairy products, such as starter cultures in food supplements and in foods considered functional. In vitro gut fermentation models present an unmatched opportunity of performing studies frequently allenged in humans and animals owing to ethical concerns. A dynamic model of the human intestinal microbial ecosystem (SHIME) was designed to better simulate conditions intestinal microbiota. Methods: The SHIME model was used to study the effect of Enterococuus faecium CRL 183 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2 wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota were monitored. The microbiota were then subjected to a 4-wk treatment period by adding 108 CFU/mL of the Enterococcus faecium CRL 183 to vessel one (the stomach compartment). Results: The addition resulted into an overall increase of bacterial marker populations (Enterobacteriaceae, Lactobacillus spp., Bifidobacterium spp. and Clostridium spp.), with a significant increase of the Lactobacillus sp. and Bifidobacterium sp populations. The short-chain fatty acid (SCFA) concentration increased during the supplementation period; this was due mainly to a significant increase in the levels of acetic, butyric and propionic acids. Ammonium concentrations increased during the supplementation period. Conclusions: Results showed that the major effect of E. faecium CRL 183 was found in the ascendant and transverse colon.
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spelling Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183EnterococcusGastrointestinal resource managementGut microbiotaSimulator of Human Intestinal Microbial Ecosystem (SHIME)Background: Enterococci are used in a large number of dairy products, such as starter cultures in food supplements and in foods considered functional. In vitro gut fermentation models present an unmatched opportunity of performing studies frequently allenged in humans and animals owing to ethical concerns. A dynamic model of the human intestinal microbial ecosystem (SHIME) was designed to better simulate conditions intestinal microbiota. Methods: The SHIME model was used to study the effect of Enterococuus faecium CRL 183 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2 wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota were monitored. The microbiota were then subjected to a 4-wk treatment period by adding 108 CFU/mL of the Enterococcus faecium CRL 183 to vessel one (the stomach compartment). Results: The addition resulted into an overall increase of bacterial marker populations (Enterobacteriaceae, Lactobacillus spp., Bifidobacterium spp. and Clostridium spp.), with a significant increase of the Lactobacillus sp. and Bifidobacterium sp populations. The short-chain fatty acid (SCFA) concentration increased during the supplementation period; this was due mainly to a significant increase in the levels of acetic, butyric and propionic acids. Ammonium concentrations increased during the supplementation period. Conclusions: Results showed that the major effect of E. faecium CRL 183 was found in the ascendant and transverse colon.Department of Food and Nutrition Laboratory Study of Probiotics and Prebiotics Faculty of Pharmaceutical Sciences of Araraquara UNESP-Paulista State UniversitySchool of Engineering of São Carlos University of São PauloDepartment of Food and Nutrition Laboratory Study of Probiotics and Prebiotics Faculty of Pharmaceutical Sciences of Araraquara UNESP-Paulista State UniversityUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Sivieri, Katia [UNESP]Bianchi, Fernanda [UNESP]Tallarico, Maria A.Rossi, Elizeu A. [UNESP]2022-04-29T05:37:13Z2022-04-29T05:37:13Z2011-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article389-402http://dx.doi.org/10.31989/ffhd.v1i10.119Functional Foods in Health and Disease, v. 1, n. 10, p. 389-402, 2011.2160-38552378-7007http://hdl.handle.net/11449/22701510.31989/ffhd.v1i10.1192-s2.0-84867871226Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFunctional Foods in Health and Diseaseinfo:eu-repo/semantics/openAccess2024-06-21T12:46:40Zoai:repositorio.unesp.br:11449/227015Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:23:53.859939Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
title Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
spellingShingle Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
Sivieri, Katia [UNESP]
Enterococcus
Gastrointestinal resource management
Gut microbiota
Simulator of Human Intestinal Microbial Ecosystem (SHIME)
title_short Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
title_full Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
title_fullStr Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
title_full_unstemmed Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
title_sort Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
author Sivieri, Katia [UNESP]
author_facet Sivieri, Katia [UNESP]
Bianchi, Fernanda [UNESP]
Tallarico, Maria A.
Rossi, Elizeu A. [UNESP]
author_role author
author2 Bianchi, Fernanda [UNESP]
Tallarico, Maria A.
Rossi, Elizeu A. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Sivieri, Katia [UNESP]
Bianchi, Fernanda [UNESP]
Tallarico, Maria A.
Rossi, Elizeu A. [UNESP]
dc.subject.por.fl_str_mv Enterococcus
Gastrointestinal resource management
Gut microbiota
Simulator of Human Intestinal Microbial Ecosystem (SHIME)
topic Enterococcus
Gastrointestinal resource management
Gut microbiota
Simulator of Human Intestinal Microbial Ecosystem (SHIME)
description Background: Enterococci are used in a large number of dairy products, such as starter cultures in food supplements and in foods considered functional. In vitro gut fermentation models present an unmatched opportunity of performing studies frequently allenged in humans and animals owing to ethical concerns. A dynamic model of the human intestinal microbial ecosystem (SHIME) was designed to better simulate conditions intestinal microbiota. Methods: The SHIME model was used to study the effect of Enterococuus faecium CRL 183 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2 wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota were monitored. The microbiota were then subjected to a 4-wk treatment period by adding 108 CFU/mL of the Enterococcus faecium CRL 183 to vessel one (the stomach compartment). Results: The addition resulted into an overall increase of bacterial marker populations (Enterobacteriaceae, Lactobacillus spp., Bifidobacterium spp. and Clostridium spp.), with a significant increase of the Lactobacillus sp. and Bifidobacterium sp populations. The short-chain fatty acid (SCFA) concentration increased during the supplementation period; this was due mainly to a significant increase in the levels of acetic, butyric and propionic acids. Ammonium concentrations increased during the supplementation period. Conclusions: Results showed that the major effect of E. faecium CRL 183 was found in the ascendant and transverse colon.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-01
2022-04-29T05:37:13Z
2022-04-29T05:37:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.31989/ffhd.v1i10.119
Functional Foods in Health and Disease, v. 1, n. 10, p. 389-402, 2011.
2160-3855
2378-7007
http://hdl.handle.net/11449/227015
10.31989/ffhd.v1i10.119
2-s2.0-84867871226
url http://dx.doi.org/10.31989/ffhd.v1i10.119
http://hdl.handle.net/11449/227015
identifier_str_mv Functional Foods in Health and Disease, v. 1, n. 10, p. 389-402, 2011.
2160-3855
2378-7007
10.31989/ffhd.v1i10.119
2-s2.0-84867871226
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Functional Foods in Health and Disease
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 389-402
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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