Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2018.10.008 http://hdl.handle.net/11449/188354 |
Resumo: | Studies involving different methods and techniques of cryopreservation and its interactions with the conception rates in artificial insemination (AI) programs are reported in the literature. This study evaluated the sperm kinetics, plasma membrane integrity, and fertility rates of mares inseminated with cryopreserved stallion semen subjected to different freezing methods. For this, four ejaculates from five stallions were collected and frozen in conventional (Styrofoam box) or automated system in Mini-Digitcool ZH 400. Seminal samples were evaluated after thawing for sperm motion parameters by CASA and plasma membrane integrity by epifluorescence microscopy. For the fertility trial, a cross-over model was performed using 100 cycles of 50 mares, which were inseminated by one the two freezing methods. No differences were observed for sperm motion parameters and plasma membrane integrity between groups (P >.05). The pregnancy rate using the conventional method was 56% (28/50) and did not differ (P =.5406) from the pregnancy rate (64%, 32/50) obtained using the automatized method. The use of semen from fertile stallions may not illustrate small differences in the two freezing methods evaluated. Conventional and automated freezing systems did not differ in the quality and viability of fertile stallion semen and conception rates, indicating that the two methodologies can be safely used in AI programs. |
id |
UNSP_89db5afd8f97010a91a1a5f471df6394 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/188354 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion SpermEquineFertilityFreezing protocolFreezing rateSemen cryopreservationStudies involving different methods and techniques of cryopreservation and its interactions with the conception rates in artificial insemination (AI) programs are reported in the literature. This study evaluated the sperm kinetics, plasma membrane integrity, and fertility rates of mares inseminated with cryopreserved stallion semen subjected to different freezing methods. For this, four ejaculates from five stallions were collected and frozen in conventional (Styrofoam box) or automated system in Mini-Digitcool ZH 400. Seminal samples were evaluated after thawing for sperm motion parameters by CASA and plasma membrane integrity by epifluorescence microscopy. For the fertility trial, a cross-over model was performed using 100 cycles of 50 mares, which were inseminated by one the two freezing methods. No differences were observed for sperm motion parameters and plasma membrane integrity between groups (P >.05). The pregnancy rate using the conventional method was 56% (28/50) and did not differ (P =.5406) from the pregnancy rate (64%, 32/50) obtained using the automatized method. The use of semen from fertile stallions may not illustrate small differences in the two freezing methods evaluated. Conventional and automated freezing systems did not differ in the quality and viability of fertile stallion semen and conception rates, indicating that the two methodologies can be safely used in AI programs.Department of Animal Reproduction Parananense UniversityDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESPDepartment of Animal Reproduction Minas Gerais Federal UniversityDepartment of Animal Science Uberaba UniversityDepartment of Animal Science Santo Amaro UniversityDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESPParananense UniversityUniversidade Estadual Paulista (Unesp)Minas Gerais Federal UniversityUberaba UniversitySanto Amaro UniversityMaziero, Rosiara Rosaria DiasGuaitolini, Carlos Renato de Freitas [UNESP]Guasti, Priscilla Nascimento [UNESP]Monteiro, Gabriel AugustoMartin, IanSilva, Juliano Pianowski Marques daCrespilho, André MacielPapa, Frederico Ozanam [UNESP]2019-10-06T16:05:23Z2019-10-06T16:05:23Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-40http://dx.doi.org/10.1016/j.jevs.2018.10.008Journal of Equine Veterinary Science, v. 72, p. 37-40.0737-0806http://hdl.handle.net/11449/18835410.1016/j.jevs.2018.10.0082-s2.0-85056470614Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-23T05:32:56Zoai:repositorio.unesp.br:11449/188354Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:32:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
title |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
spellingShingle |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm Maziero, Rosiara Rosaria Dias Equine Fertility Freezing protocol Freezing rate Semen cryopreservation |
title_short |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
title_full |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
title_fullStr |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
title_full_unstemmed |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
title_sort |
Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm |
author |
Maziero, Rosiara Rosaria Dias |
author_facet |
Maziero, Rosiara Rosaria Dias Guaitolini, Carlos Renato de Freitas [UNESP] Guasti, Priscilla Nascimento [UNESP] Monteiro, Gabriel Augusto Martin, Ian Silva, Juliano Pianowski Marques da Crespilho, André Maciel Papa, Frederico Ozanam [UNESP] |
author_role |
author |
author2 |
Guaitolini, Carlos Renato de Freitas [UNESP] Guasti, Priscilla Nascimento [UNESP] Monteiro, Gabriel Augusto Martin, Ian Silva, Juliano Pianowski Marques da Crespilho, André Maciel Papa, Frederico Ozanam [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Parananense University Universidade Estadual Paulista (Unesp) Minas Gerais Federal University Uberaba University Santo Amaro University |
dc.contributor.author.fl_str_mv |
Maziero, Rosiara Rosaria Dias Guaitolini, Carlos Renato de Freitas [UNESP] Guasti, Priscilla Nascimento [UNESP] Monteiro, Gabriel Augusto Martin, Ian Silva, Juliano Pianowski Marques da Crespilho, André Maciel Papa, Frederico Ozanam [UNESP] |
dc.subject.por.fl_str_mv |
Equine Fertility Freezing protocol Freezing rate Semen cryopreservation |
topic |
Equine Fertility Freezing protocol Freezing rate Semen cryopreservation |
description |
Studies involving different methods and techniques of cryopreservation and its interactions with the conception rates in artificial insemination (AI) programs are reported in the literature. This study evaluated the sperm kinetics, plasma membrane integrity, and fertility rates of mares inseminated with cryopreserved stallion semen subjected to different freezing methods. For this, four ejaculates from five stallions were collected and frozen in conventional (Styrofoam box) or automated system in Mini-Digitcool ZH 400. Seminal samples were evaluated after thawing for sperm motion parameters by CASA and plasma membrane integrity by epifluorescence microscopy. For the fertility trial, a cross-over model was performed using 100 cycles of 50 mares, which were inseminated by one the two freezing methods. No differences were observed for sperm motion parameters and plasma membrane integrity between groups (P >.05). The pregnancy rate using the conventional method was 56% (28/50) and did not differ (P =.5406) from the pregnancy rate (64%, 32/50) obtained using the automatized method. The use of semen from fertile stallions may not illustrate small differences in the two freezing methods evaluated. Conventional and automated freezing systems did not differ in the quality and viability of fertile stallion semen and conception rates, indicating that the two methodologies can be safely used in AI programs. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:05:23Z 2019-10-06T16:05:23Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2018.10.008 Journal of Equine Veterinary Science, v. 72, p. 37-40. 0737-0806 http://hdl.handle.net/11449/188354 10.1016/j.jevs.2018.10.008 2-s2.0-85056470614 |
url |
http://dx.doi.org/10.1016/j.jevs.2018.10.008 http://hdl.handle.net/11449/188354 |
identifier_str_mv |
Journal of Equine Veterinary Science, v. 72, p. 37-40. 0737-0806 10.1016/j.jevs.2018.10.008 2-s2.0-85056470614 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Equine Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
37-40 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964969491496960 |