Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2012.06.008 http://hdl.handle.net/11449/74671 |
Resumo: | In the present study, different freezing systems (Styrofoam box and Mini Digitcool ZH 400) and storage volumes (0.5- and 0.25-mL straws) were compared with regard to sperm kinetics and plasma membrane integrity of frozen and thawed semen. For that, three ejaculates from four animals were frozen in Styrofoam box and Mini Digitcool ZH 400 machine. The 0.5-mL straws were thawed at 46°C for 20 seconds, and the 0.25-mL straws were thawed at 46°C for 12 seconds. Statistical analysis was performed using program R of descriptive analysis box plot, followed by analysis of variance using PROC MIXED of SAS 9.1 package. Variances of 5% were considered as different. There was no interaction between the straw sizes and volumes; however, statistical differences were observed between the semen storage volumes. The 0.5-mL straws had higher total motility (%), progressive motility (%), average path velocity (μm/s), straight-line velocity (μm/s), curvilinear velocity (μm/s), and rapid sperm percentage (%) than the 0.25-mL straws. However, plasma membrane integrity analysis did not differ between the two straws. Thus, it is possible to conclude that equine sperm cryopreserved in 0.5-mL straws has better sperm kinetics than when stored in 0.25-mL straws. Additionally, it is possible to conclude that automated systems that enable faster freezing rates result in a seminal quality that is similar to the one obtained by the conventional system using Styrofoam boxes. © 2013 Elsevier Inc. |
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Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing ConditionsCryopreservationEquineFreezing systemSemenVolumeAnimaliaEquidaeIn the present study, different freezing systems (Styrofoam box and Mini Digitcool ZH 400) and storage volumes (0.5- and 0.25-mL straws) were compared with regard to sperm kinetics and plasma membrane integrity of frozen and thawed semen. For that, three ejaculates from four animals were frozen in Styrofoam box and Mini Digitcool ZH 400 machine. The 0.5-mL straws were thawed at 46°C for 20 seconds, and the 0.25-mL straws were thawed at 46°C for 12 seconds. Statistical analysis was performed using program R of descriptive analysis box plot, followed by analysis of variance using PROC MIXED of SAS 9.1 package. Variances of 5% were considered as different. There was no interaction between the straw sizes and volumes; however, statistical differences were observed between the semen storage volumes. The 0.5-mL straws had higher total motility (%), progressive motility (%), average path velocity (μm/s), straight-line velocity (μm/s), curvilinear velocity (μm/s), and rapid sperm percentage (%) than the 0.25-mL straws. However, plasma membrane integrity analysis did not differ between the two straws. Thus, it is possible to conclude that equine sperm cryopreserved in 0.5-mL straws has better sperm kinetics than when stored in 0.25-mL straws. Additionally, it is possible to conclude that automated systems that enable faster freezing rates result in a seminal quality that is similar to the one obtained by the conventional system using Styrofoam boxes. © 2013 Elsevier Inc.Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP, BotucatuDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP, BotucatuUniversidade Estadual Paulista (Unesp)Dias Maziero, Rosiara Rosária [UNESP]Guasti, Priscilla Nascimento [UNESP]Monteiro, Gabriel A. [UNESP]Avanzi, Bruno R. [UNESP]Hartwig, Felipe Pires [UNESP]Lisboa, Fernando Paixão [UNESP]Martin, Ian [UNESP]Papa, Frederico Ozanam [UNESP]2014-05-27T11:28:34Z2014-05-27T11:28:34Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article165-168application/pdfhttp://dx.doi.org/10.1016/j.jevs.2012.06.008Journal of Equine Veterinary Science, v. 33, n. 3, p. 165-168, 2013.0737-0806http://hdl.handle.net/11449/7467110.1016/j.jevs.2012.06.008WOS:0003157634000052-s2.0-848742889442-s2.0-84874288944.pdf0640158639112242Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Science0.8800,390info:eu-repo/semantics/openAccess2024-01-05T06:24:25Zoai:repositorio.unesp.br:11449/74671Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:24:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
title |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
spellingShingle |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions Dias Maziero, Rosiara Rosária [UNESP] Cryopreservation Equine Freezing system Semen Volume Animalia Equidae |
title_short |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
title_full |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
title_fullStr |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
title_full_unstemmed |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
title_sort |
Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions |
author |
Dias Maziero, Rosiara Rosária [UNESP] |
author_facet |
Dias Maziero, Rosiara Rosária [UNESP] Guasti, Priscilla Nascimento [UNESP] Monteiro, Gabriel A. [UNESP] Avanzi, Bruno R. [UNESP] Hartwig, Felipe Pires [UNESP] Lisboa, Fernando Paixão [UNESP] Martin, Ian [UNESP] Papa, Frederico Ozanam [UNESP] |
author_role |
author |
author2 |
Guasti, Priscilla Nascimento [UNESP] Monteiro, Gabriel A. [UNESP] Avanzi, Bruno R. [UNESP] Hartwig, Felipe Pires [UNESP] Lisboa, Fernando Paixão [UNESP] Martin, Ian [UNESP] Papa, Frederico Ozanam [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dias Maziero, Rosiara Rosária [UNESP] Guasti, Priscilla Nascimento [UNESP] Monteiro, Gabriel A. [UNESP] Avanzi, Bruno R. [UNESP] Hartwig, Felipe Pires [UNESP] Lisboa, Fernando Paixão [UNESP] Martin, Ian [UNESP] Papa, Frederico Ozanam [UNESP] |
dc.subject.por.fl_str_mv |
Cryopreservation Equine Freezing system Semen Volume Animalia Equidae |
topic |
Cryopreservation Equine Freezing system Semen Volume Animalia Equidae |
description |
In the present study, different freezing systems (Styrofoam box and Mini Digitcool ZH 400) and storage volumes (0.5- and 0.25-mL straws) were compared with regard to sperm kinetics and plasma membrane integrity of frozen and thawed semen. For that, three ejaculates from four animals were frozen in Styrofoam box and Mini Digitcool ZH 400 machine. The 0.5-mL straws were thawed at 46°C for 20 seconds, and the 0.25-mL straws were thawed at 46°C for 12 seconds. Statistical analysis was performed using program R of descriptive analysis box plot, followed by analysis of variance using PROC MIXED of SAS 9.1 package. Variances of 5% were considered as different. There was no interaction between the straw sizes and volumes; however, statistical differences were observed between the semen storage volumes. The 0.5-mL straws had higher total motility (%), progressive motility (%), average path velocity (μm/s), straight-line velocity (μm/s), curvilinear velocity (μm/s), and rapid sperm percentage (%) than the 0.25-mL straws. However, plasma membrane integrity analysis did not differ between the two straws. Thus, it is possible to conclude that equine sperm cryopreserved in 0.5-mL straws has better sperm kinetics than when stored in 0.25-mL straws. Additionally, it is possible to conclude that automated systems that enable faster freezing rates result in a seminal quality that is similar to the one obtained by the conventional system using Styrofoam boxes. © 2013 Elsevier Inc. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 2014-05-27T11:28:34Z 2014-05-27T11:28:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2012.06.008 Journal of Equine Veterinary Science, v. 33, n. 3, p. 165-168, 2013. 0737-0806 http://hdl.handle.net/11449/74671 10.1016/j.jevs.2012.06.008 WOS:000315763400005 2-s2.0-84874288944 2-s2.0-84874288944.pdf 0640158639112242 |
url |
http://dx.doi.org/10.1016/j.jevs.2012.06.008 http://hdl.handle.net/11449/74671 |
identifier_str_mv |
Journal of Equine Veterinary Science, v. 33, n. 3, p. 165-168, 2013. 0737-0806 10.1016/j.jevs.2012.06.008 WOS:000315763400005 2-s2.0-84874288944 2-s2.0-84874288944.pdf 0640158639112242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Equine Veterinary Science 0.880 0,390 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
165-168 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965523779256320 |