Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000468930 http://hdl.handle.net/11449/163740 |
Resumo: | Background and Aims: The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor. Methods: A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry. Results: Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4(+) neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2(+) neutrophils did not differ between groups. Conclusion: TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor. (C) 2017 S. Karger AG, Basel |
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Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm LaborPregnancyPreterm deliveryInnate immunityNeutrophilsToll-like receptorsBackground and Aims: The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor. Methods: A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry. Results: Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4(+) neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2(+) neutrophils did not differ between groups. Conclusion: TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor. (C) 2017 S. Karger AG, BaselFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Pathol, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Gynecol & Obstet, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Nursing, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Flow Cytometry Lab, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Pathol, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Gynecol & Obstet, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Nursing, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Flow Cytometry Lab, Botucatu, SP, BrazilFAPESP: 2013/12624-9FAPESP: 2011/14195-2KargerUniversidade Estadual Paulista (Unesp)Moco, Natalia Prearo [UNESP]Camargo Batista, Renata Aparecida [UNESP]Martin, Laura Fernandes [UNESP]Oliveira, Leandro Gustavo de [UNESP]Garcia de Lima Parada, Cristina Maria [UNESP]Dias-Melicio, Luciane Alarcao [UNESP]Golim, Marjorie de Assis [UNESP]Silva, Marcia Guimaraes da [UNESP]2018-11-26T17:44:46Z2018-11-26T17:44:46Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8http://dx.doi.org/10.1159/000468930Gynecologic And Obstetric Investigation. Basel: Karger, v. 83, n. 1, p. 1-8, 2018.0378-7346http://hdl.handle.net/11449/16374010.1159/000468930WOS:0004228467000010720794820325913Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGynecologic And Obstetric Investigation0,545info:eu-repo/semantics/openAccess2024-09-03T13:17:54Zoai:repositorio.unesp.br:11449/163740Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:17:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
title |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
spellingShingle |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor Moco, Natalia Prearo [UNESP] Pregnancy Preterm delivery Innate immunity Neutrophils Toll-like receptors |
title_short |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
title_full |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
title_fullStr |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
title_full_unstemmed |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
title_sort |
Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor |
author |
Moco, Natalia Prearo [UNESP] |
author_facet |
Moco, Natalia Prearo [UNESP] Camargo Batista, Renata Aparecida [UNESP] Martin, Laura Fernandes [UNESP] Oliveira, Leandro Gustavo de [UNESP] Garcia de Lima Parada, Cristina Maria [UNESP] Dias-Melicio, Luciane Alarcao [UNESP] Golim, Marjorie de Assis [UNESP] Silva, Marcia Guimaraes da [UNESP] |
author_role |
author |
author2 |
Camargo Batista, Renata Aparecida [UNESP] Martin, Laura Fernandes [UNESP] Oliveira, Leandro Gustavo de [UNESP] Garcia de Lima Parada, Cristina Maria [UNESP] Dias-Melicio, Luciane Alarcao [UNESP] Golim, Marjorie de Assis [UNESP] Silva, Marcia Guimaraes da [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moco, Natalia Prearo [UNESP] Camargo Batista, Renata Aparecida [UNESP] Martin, Laura Fernandes [UNESP] Oliveira, Leandro Gustavo de [UNESP] Garcia de Lima Parada, Cristina Maria [UNESP] Dias-Melicio, Luciane Alarcao [UNESP] Golim, Marjorie de Assis [UNESP] Silva, Marcia Guimaraes da [UNESP] |
dc.subject.por.fl_str_mv |
Pregnancy Preterm delivery Innate immunity Neutrophils Toll-like receptors |
topic |
Pregnancy Preterm delivery Innate immunity Neutrophils Toll-like receptors |
description |
Background and Aims: The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor. Methods: A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry. Results: Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4(+) neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2(+) neutrophils did not differ between groups. Conclusion: TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor. (C) 2017 S. Karger AG, Basel |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:44:46Z 2018-11-26T17:44:46Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000468930 Gynecologic And Obstetric Investigation. Basel: Karger, v. 83, n. 1, p. 1-8, 2018. 0378-7346 http://hdl.handle.net/11449/163740 10.1159/000468930 WOS:000422846700001 0720794820325913 |
url |
http://dx.doi.org/10.1159/000468930 http://hdl.handle.net/11449/163740 |
identifier_str_mv |
Gynecologic And Obstetric Investigation. Basel: Karger, v. 83, n. 1, p. 1-8, 2018. 0378-7346 10.1159/000468930 WOS:000422846700001 0720794820325913 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gynecologic And Obstetric Investigation 0,545 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021399711973376 |