Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor

Detalhes bibliográficos
Autor(a) principal: Moco, Natalia Prearo [UNESP]
Data de Publicação: 2018
Outros Autores: Camargo Batista, Renata Aparecida [UNESP], Martin, Laura Fernandes [UNESP], Oliveira, Leandro Gustavo de [UNESP], Garcia de Lima Parada, Cristina Maria [UNESP], Dias-Melicio, Luciane Alarcao [UNESP], Golim, Marjorie de Assis [UNESP], Silva, Marcia Guimaraes da [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000468930
http://hdl.handle.net/11449/163740
Resumo: Background and Aims: The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor. Methods: A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry. Results: Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4(+) neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2(+) neutrophils did not differ between groups. Conclusion: TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor. (C) 2017 S. Karger AG, Basel
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spelling Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm LaborPregnancyPreterm deliveryInnate immunityNeutrophilsToll-like receptorsBackground and Aims: The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor. Methods: A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry. Results: Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4(+) neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2(+) neutrophils did not differ between groups. Conclusion: TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor. (C) 2017 S. Karger AG, BaselFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Pathol, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Gynecol & Obstet, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Nursing, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Flow Cytometry Lab, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Pathol, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Gynecol & Obstet, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Dept Nursing, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu Blood Ctr, Flow Cytometry Lab, Botucatu, SP, BrazilFAPESP: 2013/12624-9FAPESP: 2011/14195-2KargerUniversidade Estadual Paulista (Unesp)Moco, Natalia Prearo [UNESP]Camargo Batista, Renata Aparecida [UNESP]Martin, Laura Fernandes [UNESP]Oliveira, Leandro Gustavo de [UNESP]Garcia de Lima Parada, Cristina Maria [UNESP]Dias-Melicio, Luciane Alarcao [UNESP]Golim, Marjorie de Assis [UNESP]Silva, Marcia Guimaraes da [UNESP]2018-11-26T17:44:46Z2018-11-26T17:44:46Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8http://dx.doi.org/10.1159/000468930Gynecologic And Obstetric Investigation. Basel: Karger, v. 83, n. 1, p. 1-8, 2018.0378-7346http://hdl.handle.net/11449/16374010.1159/000468930WOS:0004228467000010720794820325913Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGynecologic And Obstetric Investigation0,545info:eu-repo/semantics/openAccess2024-09-03T13:17:54Zoai:repositorio.unesp.br:11449/163740Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:17:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
title Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
spellingShingle Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
Moco, Natalia Prearo [UNESP]
Pregnancy
Preterm delivery
Innate immunity
Neutrophils
Toll-like receptors
title_short Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
title_full Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
title_fullStr Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
title_full_unstemmed Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
title_sort Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor
author Moco, Natalia Prearo [UNESP]
author_facet Moco, Natalia Prearo [UNESP]
Camargo Batista, Renata Aparecida [UNESP]
Martin, Laura Fernandes [UNESP]
Oliveira, Leandro Gustavo de [UNESP]
Garcia de Lima Parada, Cristina Maria [UNESP]
Dias-Melicio, Luciane Alarcao [UNESP]
Golim, Marjorie de Assis [UNESP]
Silva, Marcia Guimaraes da [UNESP]
author_role author
author2 Camargo Batista, Renata Aparecida [UNESP]
Martin, Laura Fernandes [UNESP]
Oliveira, Leandro Gustavo de [UNESP]
Garcia de Lima Parada, Cristina Maria [UNESP]
Dias-Melicio, Luciane Alarcao [UNESP]
Golim, Marjorie de Assis [UNESP]
Silva, Marcia Guimaraes da [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Moco, Natalia Prearo [UNESP]
Camargo Batista, Renata Aparecida [UNESP]
Martin, Laura Fernandes [UNESP]
Oliveira, Leandro Gustavo de [UNESP]
Garcia de Lima Parada, Cristina Maria [UNESP]
Dias-Melicio, Luciane Alarcao [UNESP]
Golim, Marjorie de Assis [UNESP]
Silva, Marcia Guimaraes da [UNESP]
dc.subject.por.fl_str_mv Pregnancy
Preterm delivery
Innate immunity
Neutrophils
Toll-like receptors
topic Pregnancy
Preterm delivery
Innate immunity
Neutrophils
Toll-like receptors
description Background and Aims: The inflammatory response in preterm parturition is regulated by the innate immune system. Toll-like receptors (TLR)-2 and TLR-4 are innate immune receptors that recognize the microorganisms most frequently involved in amniotic cavity infections, which are associated with activating the inflammatory response at the maternal-fetal interface during preterm labor. This study aimed to evaluate the expression of TLR-2 and TLR-4 in maternal neutrophils in preterm labor. Methods: A cross-sectional study was conducted in the Obstetrics Care Unit of Botucatu Medical School, UNESP, Brazil. The preterm group was composed of 20 pregnant women who presented preterm labor and preterm delivery. The control group was composed of 20 nonlaboring pregnant women matched to the preterm group by gestational age. Neutrophils were isolated from peripheral blood and TLR expressions were performed by real-time polymerase chain reaction and flow cytometry. Results: Gene expressions of TLR-2 and TLR-4 in neutrophils from the preterm group were statistically higher than expressions in neutrophils from the matched control group. The percentage of TLR-4(+) neutrophils was higher in the preterm group than the matched control group, while the percentage of TLR-2(+) neutrophils did not differ between groups. Conclusion: TLR-4 expression in maternal neutrophils is associated with spontaneous preterm labor. (C) 2017 S. Karger AG, Basel
publishDate 2018
dc.date.none.fl_str_mv 2018-11-26T17:44:46Z
2018-11-26T17:44:46Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000468930
Gynecologic And Obstetric Investigation. Basel: Karger, v. 83, n. 1, p. 1-8, 2018.
0378-7346
http://hdl.handle.net/11449/163740
10.1159/000468930
WOS:000422846700001
0720794820325913
url http://dx.doi.org/10.1159/000468930
http://hdl.handle.net/11449/163740
identifier_str_mv Gynecologic And Obstetric Investigation. Basel: Karger, v. 83, n. 1, p. 1-8, 2018.
0378-7346
10.1159/000468930
WOS:000422846700001
0720794820325913
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gynecologic And Obstetric Investigation
0,545
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-8
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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