Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ph4111423 http://hdl.handle.net/11449/72789 |
Resumo: | Alchornea triplinervia (Spreng.) Muell. Arg (Euphorbiaceae) is a medicinal plant commonly used by people living in the Cerrado region of Brazil to treat gastrointestinal ulcers. We previously described the gastroprotective action of methanolic extract (ME) of Alchornea triplinervia and the ethyl acetate fraction (EAF) in increasing of prostaglandin E 2 (PGE 2) gastric levels in the mucosa. In this work we evaluated the effect of EAF in promoting the healing process in rats with acetic acid-induced gastric ulcers. In addition, toxicity was investigated during treatment with EAF. After 14 days of treatment with EAF, the potent stimulator of gastric cell proliferation contributed to the acceleration of gastric ulcer healing. Upon immunohistochemical analysis, we observed a pronounced expression of COX-2, mainly in the submucosal layer. The 14-day EAF treatment also significantly increased the number of neutrophils in the gastric mucosa regeneration area. The EAF induced angiogenesis on gastric mucosa, observed as an increase of the number of blood vessels supplying the stomach in rats treated with EAF. Oral administration for 14 days of the ethyl acetate fraction from Alchornea triplinervia accelerated the healing of gastric ulcers in rats by promoting epithelial cell proliferation, increasing the number of neutrophils and stimulation of mucus production. This fraction, which contained mainly phenolic compounds, contributed to gastric mucosa healing. © 2011 by the authors; licensee MDPI, Basel, Switzerland. |
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Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in ratsAlchornea triplinerviaAngiogenesisEuphorbiaceaeHealing ulcer effectPhenolic compoundsacetic acid ethyl esterAlchornea triplinervia extractantiulcer agentcimetidinecyclooxygenase 2plant extractunclassified drugangiogenesisanimal cellanimal experimentanimal modelanimal tissuecell proliferationcontrolled studydose responsedrug effectdrug mechanismdrug screeningEuphorbiaimmunohistochemistrymalemucus secretionneutrophil countnonhumanplant leafprotein expressionratsingle drug dosestomach mucosa injurystomach protectionstomach ulcertissue regenerationtoxicity testingulcer healingulcerogenesisAlchornea triplinervia (Spreng.) Muell. Arg (Euphorbiaceae) is a medicinal plant commonly used by people living in the Cerrado region of Brazil to treat gastrointestinal ulcers. We previously described the gastroprotective action of methanolic extract (ME) of Alchornea triplinervia and the ethyl acetate fraction (EAF) in increasing of prostaglandin E 2 (PGE 2) gastric levels in the mucosa. In this work we evaluated the effect of EAF in promoting the healing process in rats with acetic acid-induced gastric ulcers. In addition, toxicity was investigated during treatment with EAF. After 14 days of treatment with EAF, the potent stimulator of gastric cell proliferation contributed to the acceleration of gastric ulcer healing. Upon immunohistochemical analysis, we observed a pronounced expression of COX-2, mainly in the submucosal layer. The 14-day EAF treatment also significantly increased the number of neutrophils in the gastric mucosa regeneration area. The EAF induced angiogenesis on gastric mucosa, observed as an increase of the number of blood vessels supplying the stomach in rats treated with EAF. Oral administration for 14 days of the ethyl acetate fraction from Alchornea triplinervia accelerated the healing of gastric ulcers in rats by promoting epithelial cell proliferation, increasing the number of neutrophils and stimulation of mucus production. This fraction, which contained mainly phenolic compounds, contributed to gastric mucosa healing. © 2011 by the authors; licensee MDPI, Basel, Switzerland.Department of Physiology Univ. Estadual Paulista-UNESP, Rubião Junior, cp 510, CEP 18618-000, Botucatu, São PauloInstitute of Chemistry Univ. Estadual Paulista-UNESP, cp 355, CEP 14801-970, Araraquara, São PauloDepartment of Morphology Univ. Estadual Paulista-UNESP, cp 610, CEP 18618-000, UNESP, Botucatu, São PauloDepartment of Physiology Univ. Estadual Paulista-UNESP, Rubião Junior, cp 510, CEP 18618-000, Botucatu, São PauloInstitute of Chemistry Univ. Estadual Paulista-UNESP, cp 355, CEP 14801-970, Araraquara, São PauloDepartment of Morphology Univ. Estadual Paulista-UNESP, cp 610, CEP 18618-000, UNESP, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)Lima, Zeila P. [UNESP]Bonamin, Flavia [UNESP]Calvo, Tamara R. [UNESP]Vilegas, Wagner [UNESP]Santos, Lourdes C. [UNESP]Rozza, Ariane L. [UNESP]Pellizzon, Claudia H. [UNESP]Rocha, Lucia R. M. [UNESP]Hiruma-Lima, Clélia A. [UNESP]2014-05-27T11:26:07Z2014-05-27T11:26:07Z2011-11-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1423-1433application/pdfhttp://dx.doi.org/10.3390/ph4111423Pharmaceuticals, v. 4, n. 11, p. 1423-1433, 2011.1424-8247http://hdl.handle.net/11449/7278910.3390/ph41114232-s2.0-804551312712-s2.0-80455131271.pdf001939377980106938145049013868440000-0002-8645-37770000-0003-3032-25560000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceuticals1,293info:eu-repo/semantics/openAccess2023-11-27T06:11:35Zoai:repositorio.unesp.br:11449/72789Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-27T06:11:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
title |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
spellingShingle |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats Lima, Zeila P. [UNESP] Alchornea triplinervia Angiogenesis Euphorbiaceae Healing ulcer effect Phenolic compounds acetic acid ethyl ester Alchornea triplinervia extract antiulcer agent cimetidine cyclooxygenase 2 plant extract unclassified drug angiogenesis animal cell animal experiment animal model animal tissue cell proliferation controlled study dose response drug effect drug mechanism drug screening Euphorbia immunohistochemistry male mucus secretion neutrophil count nonhuman plant leaf protein expression rat single drug dose stomach mucosa injury stomach protection stomach ulcer tissue regeneration toxicity testing ulcer healing ulcerogenesis |
title_short |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
title_full |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
title_fullStr |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
title_full_unstemmed |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
title_sort |
Effects of the ethyl acetate fraction of Alchornea triplinervia on healing gastric ulcer in rats |
author |
Lima, Zeila P. [UNESP] |
author_facet |
Lima, Zeila P. [UNESP] Bonamin, Flavia [UNESP] Calvo, Tamara R. [UNESP] Vilegas, Wagner [UNESP] Santos, Lourdes C. [UNESP] Rozza, Ariane L. [UNESP] Pellizzon, Claudia H. [UNESP] Rocha, Lucia R. M. [UNESP] Hiruma-Lima, Clélia A. [UNESP] |
author_role |
author |
author2 |
Bonamin, Flavia [UNESP] Calvo, Tamara R. [UNESP] Vilegas, Wagner [UNESP] Santos, Lourdes C. [UNESP] Rozza, Ariane L. [UNESP] Pellizzon, Claudia H. [UNESP] Rocha, Lucia R. M. [UNESP] Hiruma-Lima, Clélia A. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lima, Zeila P. [UNESP] Bonamin, Flavia [UNESP] Calvo, Tamara R. [UNESP] Vilegas, Wagner [UNESP] Santos, Lourdes C. [UNESP] Rozza, Ariane L. [UNESP] Pellizzon, Claudia H. [UNESP] Rocha, Lucia R. M. [UNESP] Hiruma-Lima, Clélia A. [UNESP] |
dc.subject.por.fl_str_mv |
Alchornea triplinervia Angiogenesis Euphorbiaceae Healing ulcer effect Phenolic compounds acetic acid ethyl ester Alchornea triplinervia extract antiulcer agent cimetidine cyclooxygenase 2 plant extract unclassified drug angiogenesis animal cell animal experiment animal model animal tissue cell proliferation controlled study dose response drug effect drug mechanism drug screening Euphorbia immunohistochemistry male mucus secretion neutrophil count nonhuman plant leaf protein expression rat single drug dose stomach mucosa injury stomach protection stomach ulcer tissue regeneration toxicity testing ulcer healing ulcerogenesis |
topic |
Alchornea triplinervia Angiogenesis Euphorbiaceae Healing ulcer effect Phenolic compounds acetic acid ethyl ester Alchornea triplinervia extract antiulcer agent cimetidine cyclooxygenase 2 plant extract unclassified drug angiogenesis animal cell animal experiment animal model animal tissue cell proliferation controlled study dose response drug effect drug mechanism drug screening Euphorbia immunohistochemistry male mucus secretion neutrophil count nonhuman plant leaf protein expression rat single drug dose stomach mucosa injury stomach protection stomach ulcer tissue regeneration toxicity testing ulcer healing ulcerogenesis |
description |
Alchornea triplinervia (Spreng.) Muell. Arg (Euphorbiaceae) is a medicinal plant commonly used by people living in the Cerrado region of Brazil to treat gastrointestinal ulcers. We previously described the gastroprotective action of methanolic extract (ME) of Alchornea triplinervia and the ethyl acetate fraction (EAF) in increasing of prostaglandin E 2 (PGE 2) gastric levels in the mucosa. In this work we evaluated the effect of EAF in promoting the healing process in rats with acetic acid-induced gastric ulcers. In addition, toxicity was investigated during treatment with EAF. After 14 days of treatment with EAF, the potent stimulator of gastric cell proliferation contributed to the acceleration of gastric ulcer healing. Upon immunohistochemical analysis, we observed a pronounced expression of COX-2, mainly in the submucosal layer. The 14-day EAF treatment also significantly increased the number of neutrophils in the gastric mucosa regeneration area. The EAF induced angiogenesis on gastric mucosa, observed as an increase of the number of blood vessels supplying the stomach in rats treated with EAF. Oral administration for 14 days of the ethyl acetate fraction from Alchornea triplinervia accelerated the healing of gastric ulcers in rats by promoting epithelial cell proliferation, increasing the number of neutrophils and stimulation of mucus production. This fraction, which contained mainly phenolic compounds, contributed to gastric mucosa healing. © 2011 by the authors; licensee MDPI, Basel, Switzerland. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-09 2014-05-27T11:26:07Z 2014-05-27T11:26:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ph4111423 Pharmaceuticals, v. 4, n. 11, p. 1423-1433, 2011. 1424-8247 http://hdl.handle.net/11449/72789 10.3390/ph4111423 2-s2.0-80455131271 2-s2.0-80455131271.pdf 0019393779801069 3814504901386844 0000-0002-8645-3777 0000-0003-3032-2556 0000-0002-4494-4180 |
url |
http://dx.doi.org/10.3390/ph4111423 http://hdl.handle.net/11449/72789 |
identifier_str_mv |
Pharmaceuticals, v. 4, n. 11, p. 1423-1433, 2011. 1424-8247 10.3390/ph4111423 2-s2.0-80455131271 2-s2.0-80455131271.pdf 0019393779801069 3814504901386844 0000-0002-8645-3777 0000-0003-3032-2556 0000-0002-4494-4180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceuticals 1,293 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1423-1433 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965084716367872 |