Genetic polymorphism of Streptococcus mutans in Brazilian family members
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/195839 |
Resumo: | The aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different Clones Of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-(20)agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as template in PCR reactions and the amplified DNA fragments obtained were compared by agarose gel electrophoresis. Results demonstrated that the father shared the baby's genotype in three families and the mother shared the baby's genotype in 12 families seven babies harbored Streptococcus mutans strains similar to those of their siblings. The technique was able to demonstrate the genetic Streptococcus mutans in Brazilian family members. |
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Repositório Institucional da UNESP |
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Genetic polymorphism of Streptococcus mutans in Brazilian family membersStreptococcus mutansAP-PCRgenetic polymorphismThe aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different Clones Of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-(20)agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as template in PCR reactions and the amplified DNA fragments obtained were compared by agarose gel electrophoresis. Results demonstrated that the father shared the baby's genotype in three families and the mother shared the baby's genotype in 12 families seven babies harbored Streptococcus mutans strains similar to those of their siblings. The technique was able to demonstrate the genetic Streptococcus mutans in Brazilian family members.Univ Estadual Paulista Julio Mesquita Filho, Fac Odontol, Dept Fisiol & Patol, BR-14801903 Araraquara, SP, BrazilUniv Estadual Campinas, Fac Odontol Piracicaba, Dept Biol & Patol Buco Dent, Piracicaba, SP, BrazilUniv Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmceut Araraquara, Dept Anal Clin, BR-14801903 Araraquara, SP, BrazilUniv Estadual Paulista Julio Mesquita Filho, Fac Odontol, Dept Fisiol & Patol, BR-14801903 Araraquara, SP, BrazilUniv Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmceut Araraquara, Dept Anal Clin, BR-14801903 Araraquara, SP, BrazilSoc Brasileira MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Spolidorio, DMPHofling, J. F.Pizzolitto, A. C.Rosa, E. A.Negrini, T. D.Spolidorio, L. C.2020-12-10T18:05:05Z2020-12-10T18:05:05Z2003-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article213-217Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 34, n. 3, p. 213-217, 2003.1517-8382http://hdl.handle.net/11449/195839WOS:000234802300006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2024-06-21T15:19:32Zoai:repositorio.unesp.br:11449/195839Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-21T15:19:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
title |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
spellingShingle |
Genetic polymorphism of Streptococcus mutans in Brazilian family members Spolidorio, DMP Streptococcus mutans AP-PCR genetic polymorphism |
title_short |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
title_full |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
title_fullStr |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
title_full_unstemmed |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
title_sort |
Genetic polymorphism of Streptococcus mutans in Brazilian family members |
author |
Spolidorio, DMP |
author_facet |
Spolidorio, DMP Hofling, J. F. Pizzolitto, A. C. Rosa, E. A. Negrini, T. D. Spolidorio, L. C. |
author_role |
author |
author2 |
Hofling, J. F. Pizzolitto, A. C. Rosa, E. A. Negrini, T. D. Spolidorio, L. C. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Spolidorio, DMP Hofling, J. F. Pizzolitto, A. C. Rosa, E. A. Negrini, T. D. Spolidorio, L. C. |
dc.subject.por.fl_str_mv |
Streptococcus mutans AP-PCR genetic polymorphism |
topic |
Streptococcus mutans AP-PCR genetic polymorphism |
description |
The aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different Clones Of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-(20)agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as template in PCR reactions and the amplified DNA fragments obtained were compared by agarose gel electrophoresis. Results demonstrated that the father shared the baby's genotype in three families and the mother shared the baby's genotype in 12 families seven babies harbored Streptococcus mutans strains similar to those of their siblings. The technique was able to demonstrate the genetic Streptococcus mutans in Brazilian family members. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-07-01 2020-12-10T18:05:05Z 2020-12-10T18:05:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 34, n. 3, p. 213-217, 2003. 1517-8382 http://hdl.handle.net/11449/195839 WOS:000234802300006 |
identifier_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 34, n. 3, p. 213-217, 2003. 1517-8382 WOS:000234802300006 |
url |
http://hdl.handle.net/11449/195839 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
213-217 |
dc.publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045568471105536 |