Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4 http://hdl.handle.net/11449/71424 |
Resumo: | Aim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment. |
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Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removalDental cariesGeneticPolymerase chain reactionPolymorphismStreptococcus mutansAim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment.Department of Microbiology and Immunology Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas (Unicamp), Piracicaba (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara Universidade Estadual Paulista, Júlio de Mesquita Filho (Unesp), Araraquara (SP)Department of Clinical Analysis Faculdade de Ciências Farmacêuticas, Unesp, Araraquara (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Department of Microbiology Universidade José do Rosário Vellano, Alfenas (MG)Department of Microbiology and Immunology Faculdade de Odontologia de Piracicaba, Unicamp, Piracicaba (SP)Department of Physiology and Pathology Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara Universidade Estadual Paulista, Júlio de Mesquita Filho (Unesp), Araraquara (SP)Department of Clinical Analysis Faculdade de Ciências Farmacêuticas, Unesp, Araraquara (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Department of Physiology and Pathology Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade José do Rosário VellanoDuque, Cristiane [UNESP]de Cássia Negrini, Thais [UNESP]Sacono, Nancy Tomoko [UNESP]Gomes Boriollo, Marcelo FabianoHofling, José FranciscoHebling, Josimeri [UNESP]Palomari Spolidorio, Denise Madalena [UNESP]2014-05-27T11:24:33Z2014-05-27T11:24:33Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2-8application/pdfhttp://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4Brazilian Journal of Oral Sciences, v. 8, n. 1, p. 2-8, 2009.1677-32171677-3225http://hdl.handle.net/11449/714242-s2.0-759491302172-s2.0-75949130217.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Oral Sciences0,131info:eu-repo/semantics/openAccess2024-09-27T14:05:43Zoai:repositorio.unesp.br:11449/71424Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
title |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
spellingShingle |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal Duque, Cristiane [UNESP] Dental caries Genetic Polymerase chain reaction Polymorphism Streptococcus mutans |
title_short |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
title_full |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
title_fullStr |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
title_full_unstemmed |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
title_sort |
Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal |
author |
Duque, Cristiane [UNESP] |
author_facet |
Duque, Cristiane [UNESP] de Cássia Negrini, Thais [UNESP] Sacono, Nancy Tomoko [UNESP] Gomes Boriollo, Marcelo Fabiano Hofling, José Francisco Hebling, Josimeri [UNESP] Palomari Spolidorio, Denise Madalena [UNESP] |
author_role |
author |
author2 |
de Cássia Negrini, Thais [UNESP] Sacono, Nancy Tomoko [UNESP] Gomes Boriollo, Marcelo Fabiano Hofling, José Francisco Hebling, Josimeri [UNESP] Palomari Spolidorio, Denise Madalena [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Universidade José do Rosário Vellano |
dc.contributor.author.fl_str_mv |
Duque, Cristiane [UNESP] de Cássia Negrini, Thais [UNESP] Sacono, Nancy Tomoko [UNESP] Gomes Boriollo, Marcelo Fabiano Hofling, José Francisco Hebling, Josimeri [UNESP] Palomari Spolidorio, Denise Madalena [UNESP] |
dc.subject.por.fl_str_mv |
Dental caries Genetic Polymerase chain reaction Polymorphism Streptococcus mutans |
topic |
Dental caries Genetic Polymerase chain reaction Polymorphism Streptococcus mutans |
description |
Aim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-01 2014-05-27T11:24:33Z 2014-05-27T11:24:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4 Brazilian Journal of Oral Sciences, v. 8, n. 1, p. 2-8, 2009. 1677-3217 1677-3225 http://hdl.handle.net/11449/71424 2-s2.0-75949130217 2-s2.0-75949130217.pdf 5651874509493617 0000-0002-2575-279X |
url |
http://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4 http://hdl.handle.net/11449/71424 |
identifier_str_mv |
Brazilian Journal of Oral Sciences, v. 8, n. 1, p. 2-8, 2009. 1677-3217 1677-3225 2-s2.0-75949130217 2-s2.0-75949130217.pdf 5651874509493617 0000-0002-2575-279X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Oral Sciences 0,131 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2-8 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546500872994816 |