Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal

Detalhes bibliográficos
Autor(a) principal: Duque, Cristiane [UNESP]
Data de Publicação: 2009
Outros Autores: de Cássia Negrini, Thais [UNESP], Sacono, Nancy Tomoko [UNESP], Gomes Boriollo, Marcelo Fabiano, Hofling, José Francisco, Hebling, Josimeri [UNESP], Palomari Spolidorio, Denise Madalena [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4
http://hdl.handle.net/11449/71424
Resumo: Aim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment.
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spelling Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removalDental cariesGeneticPolymerase chain reactionPolymorphismStreptococcus mutansAim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment.Department of Microbiology and Immunology Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas (Unicamp), Piracicaba (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara Universidade Estadual Paulista, Júlio de Mesquita Filho (Unesp), Araraquara (SP)Department of Clinical Analysis Faculdade de Ciências Farmacêuticas, Unesp, Araraquara (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Department of Microbiology Universidade José do Rosário Vellano, Alfenas (MG)Department of Microbiology and Immunology Faculdade de Odontologia de Piracicaba, Unicamp, Piracicaba (SP)Department of Physiology and Pathology Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara Universidade Estadual Paulista, Júlio de Mesquita Filho (Unesp), Araraquara (SP)Department of Clinical Analysis Faculdade de Ciências Farmacêuticas, Unesp, Araraquara (SP)Department of Orthodontics and Pediatric Dentistry Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Department of Physiology and Pathology Faculdade de Odontologia de Araraquara, Unesp, Araraquara (SP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade José do Rosário VellanoDuque, Cristiane [UNESP]de Cássia Negrini, Thais [UNESP]Sacono, Nancy Tomoko [UNESP]Gomes Boriollo, Marcelo FabianoHofling, José FranciscoHebling, Josimeri [UNESP]Palomari Spolidorio, Denise Madalena [UNESP]2014-05-27T11:24:33Z2014-05-27T11:24:33Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2-8application/pdfhttp://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4Brazilian Journal of Oral Sciences, v. 8, n. 1, p. 2-8, 2009.1677-32171677-3225http://hdl.handle.net/11449/714242-s2.0-759491302172-s2.0-75949130217.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Oral Sciences0,131info:eu-repo/semantics/openAccess2024-09-27T14:05:43Zoai:repositorio.unesp.br:11449/71424Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
title Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
spellingShingle Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
Duque, Cristiane [UNESP]
Dental caries
Genetic
Polymerase chain reaction
Polymorphism
Streptococcus mutans
title_short Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
title_full Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
title_fullStr Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
title_full_unstemmed Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
title_sort Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
author Duque, Cristiane [UNESP]
author_facet Duque, Cristiane [UNESP]
de Cássia Negrini, Thais [UNESP]
Sacono, Nancy Tomoko [UNESP]
Gomes Boriollo, Marcelo Fabiano
Hofling, José Francisco
Hebling, Josimeri [UNESP]
Palomari Spolidorio, Denise Madalena [UNESP]
author_role author
author2 de Cássia Negrini, Thais [UNESP]
Sacono, Nancy Tomoko [UNESP]
Gomes Boriollo, Marcelo Fabiano
Hofling, José Francisco
Hebling, Josimeri [UNESP]
Palomari Spolidorio, Denise Madalena [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
Universidade José do Rosário Vellano
dc.contributor.author.fl_str_mv Duque, Cristiane [UNESP]
de Cássia Negrini, Thais [UNESP]
Sacono, Nancy Tomoko [UNESP]
Gomes Boriollo, Marcelo Fabiano
Hofling, José Francisco
Hebling, Josimeri [UNESP]
Palomari Spolidorio, Denise Madalena [UNESP]
dc.subject.por.fl_str_mv Dental caries
Genetic
Polymerase chain reaction
Polymorphism
Streptococcus mutans
topic Dental caries
Genetic
Polymerase chain reaction
Polymorphism
Streptococcus mutans
description Aim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
2014-05-27T11:24:33Z
2014-05-27T11:24:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4
Brazilian Journal of Oral Sciences, v. 8, n. 1, p. 2-8, 2009.
1677-3217
1677-3225
http://hdl.handle.net/11449/71424
2-s2.0-75949130217
2-s2.0-75949130217.pdf
5651874509493617
0000-0002-2575-279X
url http://www.bibliotecadigital.unicamp.br/document/?code=43806&opt=4
http://hdl.handle.net/11449/71424
identifier_str_mv Brazilian Journal of Oral Sciences, v. 8, n. 1, p. 2-8, 2009.
1677-3217
1677-3225
2-s2.0-75949130217
2-s2.0-75949130217.pdf
5651874509493617
0000-0002-2575-279X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Oral Sciences
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2-8
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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