Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia

Detalhes bibliográficos
Autor(a) principal: Pinto-Souza, Caroline C. [UNESP]
Data de Publicação: 2021
Outros Autores: Coeli-Lacchini, Fernanda, Luizon, Marcelo R., Cavalli, Ricardo C., Lacchini, Riccardo, Sandrim, Valeria C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.niox.2021.02.003
http://hdl.handle.net/11449/207391
Resumo: Background and aims: Preeclampsia is associated with reduced nitric oxide (NO) bioavailability. Arginase is related to NO synthesis, but relatively unexplored in preeclampsia. However, no previous study has examined whether variations in ARG1 and ARG2 genes affect NO bioavailability and the risk of preeclampsia. Here, we compared the alleles and genotypes of single nucleotide polymorphisms (SNPs) in ARG1 (rs2781659; rs2781667; rs2246012; rs17599586) and ARG2 (rs3742879; rs10483801) in healthy pregnant women and preeclampsia, and examined whether these SNPs affect plasma nitrite concentrations (a marker of NO formation) in these groups. Methods: Genotypes for the ARG1 and ARG2 SNPs were determined by Taqman probe and plasma nitrite by an ozone-based chemiluminescence assay. Results: Regarding ARG1 SNPs, the GG genotype and G allele frequencies for rs2781659, and the C allele frequencies for rs2246012 were higher in preeclampsia compared to healthy pregnant women. Moreover, the GG genotype for rs2781659 and the TT genotype for rs2781667 were associated with higher plasma nitrite in healthy pregnant. We found no association of ARG2 polymorphisms with preeclampsia or nitrite levels in the study groups. Conclusions: Our results suggest that SNPs of ARG1 increase the risk of preeclampsia and modulate plasma nitrite levels in healthy pregnant women.
id UNSP_8d6407ca81475e15cebacdb0d9fe8537
oai_identifier_str oai:repositorio.unesp.br:11449/207391
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsiaArginaseGenetic polymorphismsNitric oxideNitritePreeclampsiaPregnancyBackground and aims: Preeclampsia is associated with reduced nitric oxide (NO) bioavailability. Arginase is related to NO synthesis, but relatively unexplored in preeclampsia. However, no previous study has examined whether variations in ARG1 and ARG2 genes affect NO bioavailability and the risk of preeclampsia. Here, we compared the alleles and genotypes of single nucleotide polymorphisms (SNPs) in ARG1 (rs2781659; rs2781667; rs2246012; rs17599586) and ARG2 (rs3742879; rs10483801) in healthy pregnant women and preeclampsia, and examined whether these SNPs affect plasma nitrite concentrations (a marker of NO formation) in these groups. Methods: Genotypes for the ARG1 and ARG2 SNPs were determined by Taqman probe and plasma nitrite by an ozone-based chemiluminescence assay. Results: Regarding ARG1 SNPs, the GG genotype and G allele frequencies for rs2781659, and the C allele frequencies for rs2246012 were higher in preeclampsia compared to healthy pregnant women. Moreover, the GG genotype for rs2781659 and the TT genotype for rs2781667 were associated with higher plasma nitrite in healthy pregnant. We found no association of ARG2 polymorphisms with preeclampsia or nitrite levels in the study groups. Conclusions: Our results suggest that SNPs of ARG1 increase the risk of preeclampsia and modulate plasma nitrite levels in healthy pregnant women.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP), Distrito Rubiao JuniorDepartment of Clinical Analyses Toxicology and Food Science School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo (USP)Department of Genetics Ecology and Evolution Institute of Biological Sciences Federal University of Minas GeraisDepartment of Gynecology and Obstetrics Faculty of Medicine of Ribeirao Preto University of Sao PauloDepartment of Psychiatric Nursing and Human Sciences Ribeirao Preto School of Nursing University of Sao Paulo (USP)Department of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP), Distrito Rubiao JuniorCAPES: 001CNPq: 2014-5/305587FAPESP: 2019/07230-8Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal de Minas Gerais (UFMG)Pinto-Souza, Caroline C. [UNESP]Coeli-Lacchini, FernandaLuizon, Marcelo R.Cavalli, Ricardo C.Lacchini, RiccardoSandrim, Valeria C. [UNESP]2021-06-25T10:54:24Z2021-06-25T10:54:24Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-25http://dx.doi.org/10.1016/j.niox.2021.02.003Nitric Oxide - Biology and Chemistry, v. 109-110, p. 20-25.1089-86111089-8603http://hdl.handle.net/11449/20739110.1016/j.niox.2021.02.0032-s2.0-85102015902Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNitric Oxide - Biology and Chemistryinfo:eu-repo/semantics/openAccess2021-10-22T12:11:08Zoai:repositorio.unesp.br:11449/207391Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:45:12.244098Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
title Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
spellingShingle Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
Pinto-Souza, Caroline C. [UNESP]
Arginase
Genetic polymorphisms
Nitric oxide
Nitrite
Preeclampsia
Pregnancy
title_short Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
title_full Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
title_fullStr Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
title_full_unstemmed Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
title_sort Effects of arginase genetic polymorphisms on nitric oxide formation in healthy pregnancy and in preeclampsia
author Pinto-Souza, Caroline C. [UNESP]
author_facet Pinto-Souza, Caroline C. [UNESP]
Coeli-Lacchini, Fernanda
Luizon, Marcelo R.
Cavalli, Ricardo C.
Lacchini, Riccardo
Sandrim, Valeria C. [UNESP]
author_role author
author2 Coeli-Lacchini, Fernanda
Luizon, Marcelo R.
Cavalli, Ricardo C.
Lacchini, Riccardo
Sandrim, Valeria C. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Universidade Federal de Minas Gerais (UFMG)
dc.contributor.author.fl_str_mv Pinto-Souza, Caroline C. [UNESP]
Coeli-Lacchini, Fernanda
Luizon, Marcelo R.
Cavalli, Ricardo C.
Lacchini, Riccardo
Sandrim, Valeria C. [UNESP]
dc.subject.por.fl_str_mv Arginase
Genetic polymorphisms
Nitric oxide
Nitrite
Preeclampsia
Pregnancy
topic Arginase
Genetic polymorphisms
Nitric oxide
Nitrite
Preeclampsia
Pregnancy
description Background and aims: Preeclampsia is associated with reduced nitric oxide (NO) bioavailability. Arginase is related to NO synthesis, but relatively unexplored in preeclampsia. However, no previous study has examined whether variations in ARG1 and ARG2 genes affect NO bioavailability and the risk of preeclampsia. Here, we compared the alleles and genotypes of single nucleotide polymorphisms (SNPs) in ARG1 (rs2781659; rs2781667; rs2246012; rs17599586) and ARG2 (rs3742879; rs10483801) in healthy pregnant women and preeclampsia, and examined whether these SNPs affect plasma nitrite concentrations (a marker of NO formation) in these groups. Methods: Genotypes for the ARG1 and ARG2 SNPs were determined by Taqman probe and plasma nitrite by an ozone-based chemiluminescence assay. Results: Regarding ARG1 SNPs, the GG genotype and G allele frequencies for rs2781659, and the C allele frequencies for rs2246012 were higher in preeclampsia compared to healthy pregnant women. Moreover, the GG genotype for rs2781659 and the TT genotype for rs2781667 were associated with higher plasma nitrite in healthy pregnant. We found no association of ARG2 polymorphisms with preeclampsia or nitrite levels in the study groups. Conclusions: Our results suggest that SNPs of ARG1 increase the risk of preeclampsia and modulate plasma nitrite levels in healthy pregnant women.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:54:24Z
2021-06-25T10:54:24Z
2021-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.niox.2021.02.003
Nitric Oxide - Biology and Chemistry, v. 109-110, p. 20-25.
1089-8611
1089-8603
http://hdl.handle.net/11449/207391
10.1016/j.niox.2021.02.003
2-s2.0-85102015902
url http://dx.doi.org/10.1016/j.niox.2021.02.003
http://hdl.handle.net/11449/207391
identifier_str_mv Nitric Oxide - Biology and Chemistry, v. 109-110, p. 20-25.
1089-8611
1089-8603
10.1016/j.niox.2021.02.003
2-s2.0-85102015902
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nitric Oxide - Biology and Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20-25
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129354440376320