Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2017/4916971 http://hdl.handle.net/11449/176212 |
Resumo: | Ghrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a expression in human periodontal cells challenged with the periodontopathogen Fusobacterium nucleatum, in gingival biopsies from periodontally healthy and diseased individuals, and from rats with and without ligature-induced periodontitis was analyzed by real-time PCR, immunocytochemistry, and immunofluorescence. F. nucleatum induced an initial upregulation and subsequent downregulation of GHS-R1a in periodontal cells. In rat experimental periodontitis, the GHS-R1a expression at periodontitis sites was increased during the early stage of periodontitis, but significantly reduced afterwards, when compared with healthy sites. In human gingival biopsies, periodontally diseased sites showed a significantly lower GHS-R1a expression than the healthy sites. The expression of the functional ghrelin receptor in periodontal cells and tissues is modulated by periodontal bacteria. Due to the downregulation of the functional ghrelin receptor by long-term exposure to periodontal bacteria, the anti-inflammatory actions of ghrelin may be diminished in chronic periodontal infections, which could lead to an enhanced periodontal inflammation and tissue destruction. |
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Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in VivoGhrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a expression in human periodontal cells challenged with the periodontopathogen Fusobacterium nucleatum, in gingival biopsies from periodontally healthy and diseased individuals, and from rats with and without ligature-induced periodontitis was analyzed by real-time PCR, immunocytochemistry, and immunofluorescence. F. nucleatum induced an initial upregulation and subsequent downregulation of GHS-R1a in periodontal cells. In rat experimental periodontitis, the GHS-R1a expression at periodontitis sites was increased during the early stage of periodontitis, but significantly reduced afterwards, when compared with healthy sites. In human gingival biopsies, periodontally diseased sites showed a significantly lower GHS-R1a expression than the healthy sites. The expression of the functional ghrelin receptor in periodontal cells and tissues is modulated by periodontal bacteria. Due to the downregulation of the functional ghrelin receptor by long-term exposure to periodontal bacteria, the anti-inflammatory actions of ghrelin may be diminished in chronic periodontal infections, which could lead to an enhanced periodontal inflammation and tissue destruction.Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of BonnDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University (UNESP)Department of Periodontology Laboratory of Oral Microbiology University of BernDepartment of Orthodontics Center of Dento-Maxillo-Facial Medicine University of BonnFaculty of Dentistry University of SydneyInstitute of Dental Research Westmead Centre for Oral Health and Westmead Institute for Medical ResearchDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University (UNESP)University of BonnUniversidade Estadual Paulista (Unesp)University of BernUniversity of SydneyWestmead Centre for Oral Health and Westmead Institute for Medical ResearchNokhbehsaim, MarjanDamanaki, AnnaNogueira, Andressa Vilas Boas [UNESP]Eick, SigrunMemmert, SvenjaZhou, XiaoyanNanayakkara, ShanikaGötz, WernerCirelli, Joni Augusto [UNESP]Jäger, AndreasDeschner, James2018-12-11T17:19:37Z2018-12-11T17:19:37Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2017/4916971Mediators of Inflammation, v. 2017.1466-18610962-9351http://hdl.handle.net/11449/17621210.1155/2017/49169712-s2.0-850458323122-s2.0-85045832312.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators of Inflammation1,3701,370info:eu-repo/semantics/openAccess2023-11-22T06:11:22Zoai:repositorio.unesp.br:11449/176212Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-22T06:11:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
title |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
spellingShingle |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo Nokhbehsaim, Marjan |
title_short |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
title_full |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
title_fullStr |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
title_full_unstemmed |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
title_sort |
Regulation of Ghrelin Receptor by Periodontal Bacteria in Vitro and in Vivo |
author |
Nokhbehsaim, Marjan |
author_facet |
Nokhbehsaim, Marjan Damanaki, Anna Nogueira, Andressa Vilas Boas [UNESP] Eick, Sigrun Memmert, Svenja Zhou, Xiaoyan Nanayakkara, Shanika Götz, Werner Cirelli, Joni Augusto [UNESP] Jäger, Andreas Deschner, James |
author_role |
author |
author2 |
Damanaki, Anna Nogueira, Andressa Vilas Boas [UNESP] Eick, Sigrun Memmert, Svenja Zhou, Xiaoyan Nanayakkara, Shanika Götz, Werner Cirelli, Joni Augusto [UNESP] Jäger, Andreas Deschner, James |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Bonn Universidade Estadual Paulista (Unesp) University of Bern University of Sydney Westmead Centre for Oral Health and Westmead Institute for Medical Research |
dc.contributor.author.fl_str_mv |
Nokhbehsaim, Marjan Damanaki, Anna Nogueira, Andressa Vilas Boas [UNESP] Eick, Sigrun Memmert, Svenja Zhou, Xiaoyan Nanayakkara, Shanika Götz, Werner Cirelli, Joni Augusto [UNESP] Jäger, Andreas Deschner, James |
description |
Ghrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a expression in human periodontal cells challenged with the periodontopathogen Fusobacterium nucleatum, in gingival biopsies from periodontally healthy and diseased individuals, and from rats with and without ligature-induced periodontitis was analyzed by real-time PCR, immunocytochemistry, and immunofluorescence. F. nucleatum induced an initial upregulation and subsequent downregulation of GHS-R1a in periodontal cells. In rat experimental periodontitis, the GHS-R1a expression at periodontitis sites was increased during the early stage of periodontitis, but significantly reduced afterwards, when compared with healthy sites. In human gingival biopsies, periodontally diseased sites showed a significantly lower GHS-R1a expression than the healthy sites. The expression of the functional ghrelin receptor in periodontal cells and tissues is modulated by periodontal bacteria. Due to the downregulation of the functional ghrelin receptor by long-term exposure to periodontal bacteria, the anti-inflammatory actions of ghrelin may be diminished in chronic periodontal infections, which could lead to an enhanced periodontal inflammation and tissue destruction. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:19:37Z 2018-12-11T17:19:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2017/4916971 Mediators of Inflammation, v. 2017. 1466-1861 0962-9351 http://hdl.handle.net/11449/176212 10.1155/2017/4916971 2-s2.0-85045832312 2-s2.0-85045832312.pdf |
url |
http://dx.doi.org/10.1155/2017/4916971 http://hdl.handle.net/11449/176212 |
identifier_str_mv |
Mediators of Inflammation, v. 2017. 1466-1861 0962-9351 10.1155/2017/4916971 2-s2.0-85045832312 2-s2.0-85045832312.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators of Inflammation 1,370 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965024156909568 |