Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfb.2021.111762 http://hdl.handle.net/11449/206206 |
Resumo: | The inadequate disposal and the difficulty in its removal from water treatment systems have made the endocrine disruptor bisphenol A (BPA) a significant hazard for humans and animals. The molecular-level mechanisms of BPA action are not known in detail, which calls for systematic investigations using cell membrane models. This paper shows that BPA affects Langmuir monolayers and giant unilamellar vesicles (GUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) used as membrane models, in a concentration-dependent manner and with effects that depend on BPA aggregation. BPA increases DPPC monolayer fluidity in surface pressure isotherms upon interacting with the headgroups through hydrogen bonding, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In DPPC GUVs, BPA induced wrinkling and distortion in the spherical shape of the vesicles, but this was only observed for fresh solutions where it is not aggregated. BPA also decreased the viability of hamster ovary cells (CHO) in in vitro experiments. In contrast, aged, aggregated BPA solutions did not affect the GUVs and even increased CHO viability. These results may be rationalized in terms of size-dependent effects of BPA, which may be relevant for its endocrine-disrupting effects. |
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Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viabilityBisphenol ACell membrane modelsCell viabilityEndocrine disruptorsLangmuir monolayersVesiclesThe inadequate disposal and the difficulty in its removal from water treatment systems have made the endocrine disruptor bisphenol A (BPA) a significant hazard for humans and animals. The molecular-level mechanisms of BPA action are not known in detail, which calls for systematic investigations using cell membrane models. This paper shows that BPA affects Langmuir monolayers and giant unilamellar vesicles (GUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) used as membrane models, in a concentration-dependent manner and with effects that depend on BPA aggregation. BPA increases DPPC monolayer fluidity in surface pressure isotherms upon interacting with the headgroups through hydrogen bonding, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In DPPC GUVs, BPA induced wrinkling and distortion in the spherical shape of the vesicles, but this was only observed for fresh solutions where it is not aggregated. BPA also decreased the viability of hamster ovary cells (CHO) in in vitro experiments. In contrast, aged, aggregated BPA solutions did not affect the GUVs and even increased CHO viability. These results may be rationalized in terms of size-dependent effects of BPA, which may be relevant for its endocrine-disrupting effects.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) School of Technology and Applied SciencesSão Carlos Institute of Physics University of São Paulo, CP 369São Paulo State University (UNESP) School of Technology and Applied SciencesCNPq: 304836/2018-4CNPq: 422163/2018-0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Maximino, Mateus D. [UNESP]Silva, Carla Y. [UNESP]Cavalcante, Dalita G.S.M. [UNESP]Martin, Cibely S. [UNESP]Job, Aldo E. [UNESP]Oliveira, Osvaldo N.Aléssio, Priscila [UNESP]2021-06-25T10:28:19Z2021-06-25T10:28:19Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.colsurfb.2021.111762Colloids and Surfaces B: Biointerfaces, v. 203.1873-43670927-7765http://hdl.handle.net/11449/20620610.1016/j.colsurfb.2021.1117622-s2.0-85104321348Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfacesinfo:eu-repo/semantics/openAccess2021-10-22T22:23:37Zoai:repositorio.unesp.br:11449/206206Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T22:23:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
title |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
spellingShingle |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability Maximino, Mateus D. [UNESP] Bisphenol A Cell membrane models Cell viability Endocrine disruptors Langmuir monolayers Vesicles |
title_short |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
title_full |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
title_fullStr |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
title_full_unstemmed |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
title_sort |
Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability |
author |
Maximino, Mateus D. [UNESP] |
author_facet |
Maximino, Mateus D. [UNESP] Silva, Carla Y. [UNESP] Cavalcante, Dalita G.S.M. [UNESP] Martin, Cibely S. [UNESP] Job, Aldo E. [UNESP] Oliveira, Osvaldo N. Aléssio, Priscila [UNESP] |
author_role |
author |
author2 |
Silva, Carla Y. [UNESP] Cavalcante, Dalita G.S.M. [UNESP] Martin, Cibely S. [UNESP] Job, Aldo E. [UNESP] Oliveira, Osvaldo N. Aléssio, Priscila [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Maximino, Mateus D. [UNESP] Silva, Carla Y. [UNESP] Cavalcante, Dalita G.S.M. [UNESP] Martin, Cibely S. [UNESP] Job, Aldo E. [UNESP] Oliveira, Osvaldo N. Aléssio, Priscila [UNESP] |
dc.subject.por.fl_str_mv |
Bisphenol A Cell membrane models Cell viability Endocrine disruptors Langmuir monolayers Vesicles |
topic |
Bisphenol A Cell membrane models Cell viability Endocrine disruptors Langmuir monolayers Vesicles |
description |
The inadequate disposal and the difficulty in its removal from water treatment systems have made the endocrine disruptor bisphenol A (BPA) a significant hazard for humans and animals. The molecular-level mechanisms of BPA action are not known in detail, which calls for systematic investigations using cell membrane models. This paper shows that BPA affects Langmuir monolayers and giant unilamellar vesicles (GUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) used as membrane models, in a concentration-dependent manner and with effects that depend on BPA aggregation. BPA increases DPPC monolayer fluidity in surface pressure isotherms upon interacting with the headgroups through hydrogen bonding, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In DPPC GUVs, BPA induced wrinkling and distortion in the spherical shape of the vesicles, but this was only observed for fresh solutions where it is not aggregated. BPA also decreased the viability of hamster ovary cells (CHO) in in vitro experiments. In contrast, aged, aggregated BPA solutions did not affect the GUVs and even increased CHO viability. These results may be rationalized in terms of size-dependent effects of BPA, which may be relevant for its endocrine-disrupting effects. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:28:19Z 2021-06-25T10:28:19Z 2021-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2021.111762 Colloids and Surfaces B: Biointerfaces, v. 203. 1873-4367 0927-7765 http://hdl.handle.net/11449/206206 10.1016/j.colsurfb.2021.111762 2-s2.0-85104321348 |
url |
http://dx.doi.org/10.1016/j.colsurfb.2021.111762 http://hdl.handle.net/11449/206206 |
identifier_str_mv |
Colloids and Surfaces B: Biointerfaces, v. 203. 1873-4367 0927-7765 10.1016/j.colsurfb.2021.111762 2-s2.0-85104321348 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces B: Biointerfaces |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046099123961856 |