Bisphenol A exposure induces multiple effects in DOPC membrane models

Detalhes bibliográficos
Autor(a) principal: Maximino, Mateus D. [UNESP]
Data de Publicação: 2022
Outros Autores: Martin, Cibely S. [UNESP], Aléssio, Priscila [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.molliq.2022.119253
http://hdl.handle.net/11449/240961
Resumo: Due to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in π-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations.
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spelling Bisphenol A exposure induces multiple effects in DOPC membrane modelsBisphenol ACell membrane modelsLangmuir monolayersMorphology changesVesiclesDue to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in π-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations.São Paulo State University (UNESP) School of Technology and Applied Sciences Presidente Prudente-SPSão Paulo State University (UNESP) School of Technology and Applied Sciences Presidente Prudente-SPUniversidade Estadual Paulista (UNESP)Maximino, Mateus D. [UNESP]Martin, Cibely S. [UNESP]Aléssio, Priscila [UNESP]2023-03-01T20:40:51Z2023-03-01T20:40:51Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.molliq.2022.119253Journal of Molecular Liquids, v. 359.0167-7322http://hdl.handle.net/11449/24096110.1016/j.molliq.2022.1192532-s2.0-85129755465Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Liquidsinfo:eu-repo/semantics/openAccess2024-06-18T18:18:16Zoai:repositorio.unesp.br:11449/240961Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:36:32.335369Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bisphenol A exposure induces multiple effects in DOPC membrane models
title Bisphenol A exposure induces multiple effects in DOPC membrane models
spellingShingle Bisphenol A exposure induces multiple effects in DOPC membrane models
Maximino, Mateus D. [UNESP]
Bisphenol A
Cell membrane models
Langmuir monolayers
Morphology changes
Vesicles
title_short Bisphenol A exposure induces multiple effects in DOPC membrane models
title_full Bisphenol A exposure induces multiple effects in DOPC membrane models
title_fullStr Bisphenol A exposure induces multiple effects in DOPC membrane models
title_full_unstemmed Bisphenol A exposure induces multiple effects in DOPC membrane models
title_sort Bisphenol A exposure induces multiple effects in DOPC membrane models
author Maximino, Mateus D. [UNESP]
author_facet Maximino, Mateus D. [UNESP]
Martin, Cibely S. [UNESP]
Aléssio, Priscila [UNESP]
author_role author
author2 Martin, Cibely S. [UNESP]
Aléssio, Priscila [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Maximino, Mateus D. [UNESP]
Martin, Cibely S. [UNESP]
Aléssio, Priscila [UNESP]
dc.subject.por.fl_str_mv Bisphenol A
Cell membrane models
Langmuir monolayers
Morphology changes
Vesicles
topic Bisphenol A
Cell membrane models
Langmuir monolayers
Morphology changes
Vesicles
description Due to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in π-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-01
2023-03-01T20:40:51Z
2023-03-01T20:40:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.molliq.2022.119253
Journal of Molecular Liquids, v. 359.
0167-7322
http://hdl.handle.net/11449/240961
10.1016/j.molliq.2022.119253
2-s2.0-85129755465
url http://dx.doi.org/10.1016/j.molliq.2022.119253
http://hdl.handle.net/11449/240961
identifier_str_mv Journal of Molecular Liquids, v. 359.
0167-7322
10.1016/j.molliq.2022.119253
2-s2.0-85129755465
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Molecular Liquids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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