Bisphenol A exposure induces multiple effects in DOPC membrane models
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.molliq.2022.119253 http://hdl.handle.net/11449/240961 |
Resumo: | Due to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in π-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations. |
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Bisphenol A exposure induces multiple effects in DOPC membrane modelsBisphenol ACell membrane modelsLangmuir monolayersMorphology changesVesiclesDue to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in π-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations.São Paulo State University (UNESP) School of Technology and Applied Sciences Presidente Prudente-SPSão Paulo State University (UNESP) School of Technology and Applied Sciences Presidente Prudente-SPUniversidade Estadual Paulista (UNESP)Maximino, Mateus D. [UNESP]Martin, Cibely S. [UNESP]Aléssio, Priscila [UNESP]2023-03-01T20:40:51Z2023-03-01T20:40:51Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.molliq.2022.119253Journal of Molecular Liquids, v. 359.0167-7322http://hdl.handle.net/11449/24096110.1016/j.molliq.2022.1192532-s2.0-85129755465Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Liquidsinfo:eu-repo/semantics/openAccess2024-06-18T18:18:16Zoai:repositorio.unesp.br:11449/240961Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:36:32.335369Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
title |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
spellingShingle |
Bisphenol A exposure induces multiple effects in DOPC membrane models Maximino, Mateus D. [UNESP] Bisphenol A Cell membrane models Langmuir monolayers Morphology changes Vesicles |
title_short |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
title_full |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
title_fullStr |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
title_full_unstemmed |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
title_sort |
Bisphenol A exposure induces multiple effects in DOPC membrane models |
author |
Maximino, Mateus D. [UNESP] |
author_facet |
Maximino, Mateus D. [UNESP] Martin, Cibely S. [UNESP] Aléssio, Priscila [UNESP] |
author_role |
author |
author2 |
Martin, Cibely S. [UNESP] Aléssio, Priscila [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Maximino, Mateus D. [UNESP] Martin, Cibely S. [UNESP] Aléssio, Priscila [UNESP] |
dc.subject.por.fl_str_mv |
Bisphenol A Cell membrane models Langmuir monolayers Morphology changes Vesicles |
topic |
Bisphenol A Cell membrane models Langmuir monolayers Morphology changes Vesicles |
description |
Due to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in π-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T20:40:51Z 2023-03-01T20:40:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.molliq.2022.119253 Journal of Molecular Liquids, v. 359. 0167-7322 http://hdl.handle.net/11449/240961 10.1016/j.molliq.2022.119253 2-s2.0-85129755465 |
url |
http://dx.doi.org/10.1016/j.molliq.2022.119253 http://hdl.handle.net/11449/240961 |
identifier_str_mv |
Journal of Molecular Liquids, v. 359. 0167-7322 10.1016/j.molliq.2022.119253 2-s2.0-85129755465 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Molecular Liquids |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129442510274560 |