Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/ar.a.20220 http://hdl.handle.net/11449/39639 |
Resumo: | During bone formation, as in other tissues and organs, intense cellular proliferation and differentiation are usually observed. It has been described that programmed cell death, i.e., apoptosis, takes place in the control of the cellular population by removing of the excessive and damaged cells. Although it is generally accepted that apoptotic bodies are engulfed by professional phagocytes, the neighboring cells can also take part in the removal of apoptotic bodies. In the present study, regions of initial alveolar bone formation of rat molars were examined with the aim to verify whether osteoblasts are capable of engulfing apoptotic bodies, such as professional phagocytes. Rats aged 11-19 days were sacrificed and the maxillary fragments containing the first molar were removed and immersed in the fixative solution. The specimens fixed in glutaraldehyde-formaldehyde were processed for light microscopy and transmission electron microscopy. For the detection of apoptosis, the specimens were fixed in formaldehyde, embedded in paraffin, and submitted to the TUNEL method. The results revealed round/ovoid structures containing dense bodies on the bone surface in close contact to osteoblasts and in conspicuous osteoblast vacuoles. These round/ovoid structures showed also positivity to the TUNEL method, indicating that bone cells on the bone surface are undergoing apoptosis. Ultrathin sections showed images of apoptotic bodies being engulfed by osteoblasts. Occasionally, the osteoblasts exhibited large vacuoles containing blocks of condensed chromatin and remnants of organelles. Thus, these images suggest that osteoblasts are able to engulf and degrade apoptotic bodies. (c) 2005 Wiley-Liss, Inc. |
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Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxillaosteoblastsapoptosisalveolar bonebone cellsbone formationDuring bone formation, as in other tissues and organs, intense cellular proliferation and differentiation are usually observed. It has been described that programmed cell death, i.e., apoptosis, takes place in the control of the cellular population by removing of the excessive and damaged cells. Although it is generally accepted that apoptotic bodies are engulfed by professional phagocytes, the neighboring cells can also take part in the removal of apoptotic bodies. In the present study, regions of initial alveolar bone formation of rat molars were examined with the aim to verify whether osteoblasts are capable of engulfing apoptotic bodies, such as professional phagocytes. Rats aged 11-19 days were sacrificed and the maxillary fragments containing the first molar were removed and immersed in the fixative solution. The specimens fixed in glutaraldehyde-formaldehyde were processed for light microscopy and transmission electron microscopy. For the detection of apoptosis, the specimens were fixed in formaldehyde, embedded in paraffin, and submitted to the TUNEL method. The results revealed round/ovoid structures containing dense bodies on the bone surface in close contact to osteoblasts and in conspicuous osteoblast vacuoles. These round/ovoid structures showed also positivity to the TUNEL method, indicating that bone cells on the bone surface are undergoing apoptosis. Ultrathin sections showed images of apoptotic bodies being engulfed by osteoblasts. Occasionally, the osteoblasts exhibited large vacuoles containing blocks of condensed chromatin and remnants of organelles. Thus, these images suggest that osteoblasts are able to engulf and degrade apoptotic bodies. (c) 2005 Wiley-Liss, Inc.São Paulo State Univ, Sch Dent, Dept Morphol, Lab Histol & Embryol, BR-14801903 Araraquara, SP, BrazilSão Paulo State Univ, Sch Dent, Dept Morphol, Lab Histol & Embryol, BR-14801903 Araraquara, SP, BrazilWiley-BlackwellUniversidade Estadual Paulista (Unesp)Cerri, Paulo Sérgio [UNESP]2014-05-20T15:30:12Z2014-05-20T15:30:12Z2005-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article833-840application/pdfhttp://dx.doi.org/10.1002/ar.a.20220Anatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 286A, n. 1, p. 833-840, 2005.1552-4884http://hdl.handle.net/11449/3963910.1002/ar.a.20220WOS:000231644400005WOS000231644400005.pdf327849591120788232784959112078820000-0001-5756-5828Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biologyinfo:eu-repo/semantics/openAccess2023-10-03T06:04:02Zoai:repositorio.unesp.br:11449/39639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T11:21:54.979844Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
title |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
spellingShingle |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla Cerri, Paulo Sérgio [UNESP] osteoblasts apoptosis alveolar bone bone cells bone formation |
title_short |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
title_full |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
title_fullStr |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
title_full_unstemmed |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
title_sort |
Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla |
author |
Cerri, Paulo Sérgio [UNESP] |
author_facet |
Cerri, Paulo Sérgio [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cerri, Paulo Sérgio [UNESP] |
dc.subject.por.fl_str_mv |
osteoblasts apoptosis alveolar bone bone cells bone formation |
topic |
osteoblasts apoptosis alveolar bone bone cells bone formation |
description |
During bone formation, as in other tissues and organs, intense cellular proliferation and differentiation are usually observed. It has been described that programmed cell death, i.e., apoptosis, takes place in the control of the cellular population by removing of the excessive and damaged cells. Although it is generally accepted that apoptotic bodies are engulfed by professional phagocytes, the neighboring cells can also take part in the removal of apoptotic bodies. In the present study, regions of initial alveolar bone formation of rat molars were examined with the aim to verify whether osteoblasts are capable of engulfing apoptotic bodies, such as professional phagocytes. Rats aged 11-19 days were sacrificed and the maxillary fragments containing the first molar were removed and immersed in the fixative solution. The specimens fixed in glutaraldehyde-formaldehyde were processed for light microscopy and transmission electron microscopy. For the detection of apoptosis, the specimens were fixed in formaldehyde, embedded in paraffin, and submitted to the TUNEL method. The results revealed round/ovoid structures containing dense bodies on the bone surface in close contact to osteoblasts and in conspicuous osteoblast vacuoles. These round/ovoid structures showed also positivity to the TUNEL method, indicating that bone cells on the bone surface are undergoing apoptosis. Ultrathin sections showed images of apoptotic bodies being engulfed by osteoblasts. Occasionally, the osteoblasts exhibited large vacuoles containing blocks of condensed chromatin and remnants of organelles. Thus, these images suggest that osteoblasts are able to engulf and degrade apoptotic bodies. (c) 2005 Wiley-Liss, Inc. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09-01 2014-05-20T15:30:12Z 2014-05-20T15:30:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/ar.a.20220 Anatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 286A, n. 1, p. 833-840, 2005. 1552-4884 http://hdl.handle.net/11449/39639 10.1002/ar.a.20220 WOS:000231644400005 WOS000231644400005.pdf 3278495911207882 3278495911207882 0000-0001-5756-5828 |
url |
http://dx.doi.org/10.1002/ar.a.20220 http://hdl.handle.net/11449/39639 |
identifier_str_mv |
Anatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 286A, n. 1, p. 833-840, 2005. 1552-4884 10.1002/ar.a.20220 WOS:000231644400005 WOS000231644400005.pdf 3278495911207882 0000-0001-5756-5828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
833-840 application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045809551310848 |