Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Detalhes bibliográficos
Autor(a) principal: Evaristo, Thaiane Cristine
Data de Publicação: 2014
Outros Autores: Cruzalves, Flávia Cilene Maciel Da, Moroz, Andrei[UNESP], Mion, Woner, Acorci-valério, Michele Janegitz, Felisbino, Sérgio Luis, Rossi-ferreira, Rosana, Ruiz Júnior, Raul Lopes [UNESP], Deffune, Elenice [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502014000800002
http://hdl.handle.net/11449/114466
Resumo: PURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS: This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS: Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION: The light-emitting diode is a promising tool for decellularization of soft tissues.
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spelling Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffoldsTissue EngineeringBiocompatible MaterialsQuantum DotsTracheaRabbitsPURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS: This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS: Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION: The light-emitting diode is a promising tool for decellularization of soft tissues.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Paulista State University Medical School Department of Surgery and OrthopedyHermínio Ometto University CenterUNESP School of Pharmaceutical Sciences Department of Bioprocess and BiotechnologyUNESP Medical School Blood Transfusion CenterPaulista University Department of Health ScienceUNESP Bioscience Institute Department of MorphologyUNESP Faculty of Science Department of Science BiologyUNESP Medical School Department of Surgery and OrthopedyUNESP Medical School Department of UrologyPaulista State University Medical School Department of Surgery and OrthopedyUNESP School of Pharmaceutical Sciences Department of Bioprocess and BiotechnologyUNESP Medical School Blood Transfusion CenterUNESP Bioscience Institute Department of MorphologyUNESP Faculty of Science Department of Science BiologyUNESP Medical School Department of Surgery and OrthopedyUNESP Medical School Department of UrologySociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Estadual Paulista (Unesp)Hermínio Ometto University CenterPaulista University Department of Health ScienceEvaristo, Thaiane CristineCruzalves, Flávia Cilene Maciel DaMoroz, Andrei[UNESP]Mion, WonerAcorci-valério, Michele JanegitzFelisbino, Sérgio LuisRossi-ferreira, RosanaRuiz Júnior, Raul Lopes [UNESP]Deffune, Elenice [UNESP]2015-02-02T12:39:33Z2015-02-02T12:39:33Z2014-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article485-492application/pdfhttp://dx.doi.org/10.1590/S0102-86502014000800002Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 8, p. 485-492, 2014.0102-8650http://hdl.handle.net/11449/11446610.1590/S0102-86502014000800002S0102-86502014000800485S0102-86502014000800485.pdf75668623919328169646764071339214SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2024-09-03T14:30:23Zoai:repositorio.unesp.br:11449/114466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:30:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
title Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
spellingShingle Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
Evaristo, Thaiane Cristine
Tissue Engineering
Biocompatible Materials
Quantum Dots
Trachea
Rabbits
title_short Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
title_full Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
title_fullStr Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
title_full_unstemmed Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
title_sort Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
author Evaristo, Thaiane Cristine
author_facet Evaristo, Thaiane Cristine
Cruzalves, Flávia Cilene Maciel Da
Moroz, Andrei[UNESP]
Mion, Woner
Acorci-valério, Michele Janegitz
Felisbino, Sérgio Luis
Rossi-ferreira, Rosana
Ruiz Júnior, Raul Lopes [UNESP]
Deffune, Elenice [UNESP]
author_role author
author2 Cruzalves, Flávia Cilene Maciel Da
Moroz, Andrei[UNESP]
Mion, Woner
Acorci-valério, Michele Janegitz
Felisbino, Sérgio Luis
Rossi-ferreira, Rosana
Ruiz Júnior, Raul Lopes [UNESP]
Deffune, Elenice [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Hermínio Ometto University Center
Paulista University Department of Health Science
dc.contributor.author.fl_str_mv Evaristo, Thaiane Cristine
Cruzalves, Flávia Cilene Maciel Da
Moroz, Andrei[UNESP]
Mion, Woner
Acorci-valério, Michele Janegitz
Felisbino, Sérgio Luis
Rossi-ferreira, Rosana
Ruiz Júnior, Raul Lopes [UNESP]
Deffune, Elenice [UNESP]
dc.subject.por.fl_str_mv Tissue Engineering
Biocompatible Materials
Quantum Dots
Trachea
Rabbits
topic Tissue Engineering
Biocompatible Materials
Quantum Dots
Trachea
Rabbits
description PURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS: This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS: Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION: The light-emitting diode is a promising tool for decellularization of soft tissues.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-01
2015-02-02T12:39:33Z
2015-02-02T12:39:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502014000800002
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 8, p. 485-492, 2014.
0102-8650
http://hdl.handle.net/11449/114466
10.1590/S0102-86502014000800002
S0102-86502014000800485
S0102-86502014000800485.pdf
7566862391932816
9646764071339214
url http://dx.doi.org/10.1590/S0102-86502014000800002
http://hdl.handle.net/11449/114466
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 8, p. 485-492, 2014.
0102-8650
10.1590/S0102-86502014000800002
S0102-86502014000800485
S0102-86502014000800485.pdf
7566862391932816
9646764071339214
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
0.933
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 485-492
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021417481142272

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