Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties

Detalhes bibliográficos
Autor(a) principal: Oliveira, Cláudia S.
Data de Publicação: 2018
Outros Autores: Caldeira, Cleópatra A.S., Diniz-Sousa, Rafaela, Romero, Dolores L., Marcussi, Silvana, Moura, Laura A., Fuly, André L., de Carvalho, Cicília [UNESP], Cavalcante, Walter L.G. [UNESP], Gallacci, Márcia [UNESP], Pai, Maeli Dal [UNESP], Zuliani, Juliana P., Calderon, Leonardo A., Soares, Andreimar M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s40409-018-0161-z
http://hdl.handle.net/11449/180136
Resumo: Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms.
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spelling Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor propertiesAntiophidicAntitumorBioprospectionCaribbean sea cnidariansNatural productsBackground: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms.Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio) Fundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)Brazilian Marine Biotechnology Network (BioTecMar Network)Universidade Federal de Rondônia (UNIR) Departamento de MedicinaUniversidad de La Habana Centro de Estudios de Proteínas Facultad de BiologíaUniversidade Federal de Lavras (UFLA) Departamento de QuímicaUniversidade Federal Fluminense (UFF) Departamento de Biologia Celular e Molecular (GCM) Instituto de BiologiaUniversidade Estadual Paulista (UNESP) Departamento de Farmacologia Instituto de BiociênciasUniversidade Federal de Minas Gerais (UFMG) Instituto de Ciências Biológicas Departamento de FarmacologiaUniversidade Estadual Paulista (UNESP) Departamento de Morfologia Instituto de BiociênciasCentro Universitário São Lucas (UniSL)Universidade Estadual Paulista (UNESP) Departamento de Farmacologia Instituto de BiociênciasUniversidade Estadual Paulista (UNESP) Departamento de Morfologia Instituto de BiociênciasFundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)Brazilian Marine Biotechnology Network (BioTecMar Network)Universidade Federal de Rondônia (UNIR)Facultad de BiologíaUniversidade Federal de Lavras (UFLA)Universidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Centro Universitário São Lucas (UniSL)Oliveira, Cláudia S.Caldeira, Cleópatra A.S.Diniz-Sousa, RafaelaRomero, Dolores L.Marcussi, SilvanaMoura, Laura A.Fuly, André L.de Carvalho, Cicília [UNESP]Cavalcante, Walter L.G. [UNESP]Gallacci, Márcia [UNESP]Pai, Maeli Dal [UNESP]Zuliani, Juliana P.Calderon, Leonardo A.Soares, Andreimar M.2018-12-11T17:38:18Z2018-12-11T17:38:18Z2018-08-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s40409-018-0161-zJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018.1678-91991678-9180http://hdl.handle.net/11449/18013610.1186/s40409-018-0161-zS1678-919920180001003132-s2.0-85052515898S1678-91992018000100313.pdf61713233416718219353490382598257Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases0,573info:eu-repo/semantics/openAccess2023-11-01T06:09:01Zoai:repositorio.unesp.br:11449/180136Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:35:28.201468Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
title Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
spellingShingle Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
Oliveira, Cláudia S.
Antiophidic
Antitumor
Bioprospection
Caribbean sea cnidarians
Natural products
title_short Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
title_full Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
title_fullStr Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
title_full_unstemmed Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
title_sort Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
author Oliveira, Cláudia S.
author_facet Oliveira, Cláudia S.
Caldeira, Cleópatra A.S.
Diniz-Sousa, Rafaela
Romero, Dolores L.
Marcussi, Silvana
Moura, Laura A.
Fuly, André L.
de Carvalho, Cicília [UNESP]
Cavalcante, Walter L.G. [UNESP]
Gallacci, Márcia [UNESP]
Pai, Maeli Dal [UNESP]
Zuliani, Juliana P.
Calderon, Leonardo A.
Soares, Andreimar M.
author_role author
author2 Caldeira, Cleópatra A.S.
Diniz-Sousa, Rafaela
Romero, Dolores L.
Marcussi, Silvana
Moura, Laura A.
Fuly, André L.
de Carvalho, Cicília [UNESP]
Cavalcante, Walter L.G. [UNESP]
Gallacci, Márcia [UNESP]
Pai, Maeli Dal [UNESP]
Zuliani, Juliana P.
Calderon, Leonardo A.
Soares, Andreimar M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)
Brazilian Marine Biotechnology Network (BioTecMar Network)
Universidade Federal de Rondônia (UNIR)
Facultad de Biología
Universidade Federal de Lavras (UFLA)
Universidade Federal Fluminense (UFF)
Universidade Estadual Paulista (Unesp)
Universidade Federal de Minas Gerais (UFMG)
Centro Universitário São Lucas (UniSL)
dc.contributor.author.fl_str_mv Oliveira, Cláudia S.
Caldeira, Cleópatra A.S.
Diniz-Sousa, Rafaela
Romero, Dolores L.
Marcussi, Silvana
Moura, Laura A.
Fuly, André L.
de Carvalho, Cicília [UNESP]
Cavalcante, Walter L.G. [UNESP]
Gallacci, Márcia [UNESP]
Pai, Maeli Dal [UNESP]
Zuliani, Juliana P.
Calderon, Leonardo A.
Soares, Andreimar M.
dc.subject.por.fl_str_mv Antiophidic
Antitumor
Bioprospection
Caribbean sea cnidarians
Natural products
topic Antiophidic
Antitumor
Bioprospection
Caribbean sea cnidarians
Natural products
description Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:38:18Z
2018-12-11T17:38:18Z
2018-08-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s40409-018-0161-z
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018.
1678-9199
1678-9180
http://hdl.handle.net/11449/180136
10.1186/s40409-018-0161-z
S1678-91992018000100313
2-s2.0-85052515898
S1678-91992018000100313.pdf
6171323341671821
9353490382598257
url http://dx.doi.org/10.1186/s40409-018-0161-z
http://hdl.handle.net/11449/180136
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018.
1678-9199
1678-9180
10.1186/s40409-018-0161-z
S1678-91992018000100313
2-s2.0-85052515898
S1678-91992018000100313.pdf
6171323341671821
9353490382598257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
0,573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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