Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40409-018-0161-z http://hdl.handle.net/11449/180136 |
Resumo: | Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms. |
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Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor propertiesAntiophidicAntitumorBioprospectionCaribbean sea cnidariansNatural productsBackground: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms.Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio) Fundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)Brazilian Marine Biotechnology Network (BioTecMar Network)Universidade Federal de Rondônia (UNIR) Departamento de MedicinaUniversidad de La Habana Centro de Estudios de Proteínas Facultad de BiologíaUniversidade Federal de Lavras (UFLA) Departamento de QuímicaUniversidade Federal Fluminense (UFF) Departamento de Biologia Celular e Molecular (GCM) Instituto de BiologiaUniversidade Estadual Paulista (UNESP) Departamento de Farmacologia Instituto de BiociênciasUniversidade Federal de Minas Gerais (UFMG) Instituto de Ciências Biológicas Departamento de FarmacologiaUniversidade Estadual Paulista (UNESP) Departamento de Morfologia Instituto de BiociênciasCentro Universitário São Lucas (UniSL)Universidade Estadual Paulista (UNESP) Departamento de Farmacologia Instituto de BiociênciasUniversidade Estadual Paulista (UNESP) Departamento de Morfologia Instituto de BiociênciasFundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)Brazilian Marine Biotechnology Network (BioTecMar Network)Universidade Federal de Rondônia (UNIR)Facultad de BiologíaUniversidade Federal de Lavras (UFLA)Universidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Centro Universitário São Lucas (UniSL)Oliveira, Cláudia S.Caldeira, Cleópatra A.S.Diniz-Sousa, RafaelaRomero, Dolores L.Marcussi, SilvanaMoura, Laura A.Fuly, André L.de Carvalho, Cicília [UNESP]Cavalcante, Walter L.G. [UNESP]Gallacci, Márcia [UNESP]Pai, Maeli Dal [UNESP]Zuliani, Juliana P.Calderon, Leonardo A.Soares, Andreimar M.2018-12-11T17:38:18Z2018-12-11T17:38:18Z2018-08-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s40409-018-0161-zJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018.1678-91991678-9180http://hdl.handle.net/11449/18013610.1186/s40409-018-0161-zS1678-919920180001003132-s2.0-85052515898S1678-91992018000100313.pdf61713233416718219353490382598257Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases0,573info:eu-repo/semantics/openAccess2023-11-01T06:09:01Zoai:repositorio.unesp.br:11449/180136Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:35:28.201468Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
title |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
spellingShingle |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties Oliveira, Cláudia S. Antiophidic Antitumor Bioprospection Caribbean sea cnidarians Natural products |
title_short |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
title_full |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
title_fullStr |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
title_full_unstemmed |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
title_sort |
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties |
author |
Oliveira, Cláudia S. |
author_facet |
Oliveira, Cláudia S. Caldeira, Cleópatra A.S. Diniz-Sousa, Rafaela Romero, Dolores L. Marcussi, Silvana Moura, Laura A. Fuly, André L. de Carvalho, Cicília [UNESP] Cavalcante, Walter L.G. [UNESP] Gallacci, Márcia [UNESP] Pai, Maeli Dal [UNESP] Zuliani, Juliana P. Calderon, Leonardo A. Soares, Andreimar M. |
author_role |
author |
author2 |
Caldeira, Cleópatra A.S. Diniz-Sousa, Rafaela Romero, Dolores L. Marcussi, Silvana Moura, Laura A. Fuly, André L. de Carvalho, Cicília [UNESP] Cavalcante, Walter L.G. [UNESP] Gallacci, Márcia [UNESP] Pai, Maeli Dal [UNESP] Zuliani, Juliana P. Calderon, Leonardo A. Soares, Andreimar M. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia) Brazilian Marine Biotechnology Network (BioTecMar Network) Universidade Federal de Rondônia (UNIR) Facultad de Biología Universidade Federal de Lavras (UFLA) Universidade Federal Fluminense (UFF) Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) Centro Universitário São Lucas (UniSL) |
dc.contributor.author.fl_str_mv |
Oliveira, Cláudia S. Caldeira, Cleópatra A.S. Diniz-Sousa, Rafaela Romero, Dolores L. Marcussi, Silvana Moura, Laura A. Fuly, André L. de Carvalho, Cicília [UNESP] Cavalcante, Walter L.G. [UNESP] Gallacci, Márcia [UNESP] Pai, Maeli Dal [UNESP] Zuliani, Juliana P. Calderon, Leonardo A. Soares, Andreimar M. |
dc.subject.por.fl_str_mv |
Antiophidic Antitumor Bioprospection Caribbean sea cnidarians Natural products |
topic |
Antiophidic Antitumor Bioprospection Caribbean sea cnidarians Natural products |
description |
Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:38:18Z 2018-12-11T17:38:18Z 2018-08-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40409-018-0161-z Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018. 1678-9199 1678-9180 http://hdl.handle.net/11449/180136 10.1186/s40409-018-0161-z S1678-91992018000100313 2-s2.0-85052515898 S1678-91992018000100313.pdf 6171323341671821 9353490382598257 |
url |
http://dx.doi.org/10.1186/s40409-018-0161-z http://hdl.handle.net/11449/180136 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018. 1678-9199 1678-9180 10.1186/s40409-018-0161-z S1678-91992018000100313 2-s2.0-85052515898 S1678-91992018000100313.pdf 6171323341671821 9353490382598257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128675187523584 |