Analysis of Escherichia coli isolated from bovine mastitic milk

Detalhes bibliográficos
Autor(a) principal: Rangel, Patricia
Data de Publicação: 2009
Outros Autores: Marin, José Moacir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2009000500001
http://hdl.handle.net/11449/211241
Resumo: Mastitis has been recognized for some time as the most costly disease in dairy herds. From February to November 2004, 670 samples of bovine mastitic milk from which 231 Escherichia coli strains were isolated, were collected from two Brazilian states. The strains were screened for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twenty (8.6%) strains were detected by PCR to harbor the Shiga toxin genes (8 the stx 1 gene, 12 the stx 2 gene and none both of them). Two (0.8%) of the Escherichia coli strains studied were eae positive non Shiga toxin-producing. The strains were also examined for resistance to 12 antimicrobial agents. The predominantly observed resistance was to tetracycline (92.2%), streptomycin (90.4%), nalidixic acid (88.3%), amikacin (86.5%) and cephalothin (84.8%). Multidrug resistance was found among 152 isolates (65.8%).
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spelling Analysis of Escherichia coli isolated from bovine mastitic milkAnálise de Escherichia coli isolada de leite de vacas com mastiteEscherichia colimastitisantimicrobial agentsmultidrug resistanceSTECeae geneEscherichia colimastiteagentes antimicrobianosresistência a múltiplas drogasSTECgene eaeMastitis has been recognized for some time as the most costly disease in dairy herds. From February to November 2004, 670 samples of bovine mastitic milk from which 231 Escherichia coli strains were isolated, were collected from two Brazilian states. The strains were screened for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twenty (8.6%) strains were detected by PCR to harbor the Shiga toxin genes (8 the stx 1 gene, 12 the stx 2 gene and none both of them). Two (0.8%) of the Escherichia coli strains studied were eae positive non Shiga toxin-producing. The strains were also examined for resistance to 12 antimicrobial agents. The predominantly observed resistance was to tetracycline (92.2%), streptomycin (90.4%), nalidixic acid (88.3%), amikacin (86.5%) and cephalothin (84.8%). Multidrug resistance was found among 152 isolates (65.8%).A um longo tempo a mastite tem sido reconhecida como a doença que provoca as maiores perdas econômicas nos rebanhos leiteiros. De fevereiro a novembro de 2004, foram coletadas 670 amostras de leite mastítico de vacas, provenientes de dois estados brasileiros, das quais foram isoladas 231 cepas de Escherichia coli. Estas cepas foram analisadas para a detecção dos genes de produção de Shiga toxina (stx 1 e stx 2) e do gene da intimina (eae). Vinte cepas (8,6%) foram detectadas através de PCR como contendo os genes da Shiga toxina (8 stx 1, 12 stx 2 e nenhuma delas ambos os genes). Duas cepas (0,8%) de E. coli eram eae positivo não produtoras de Shiga toxina. As cepas de E. coli foram também examinadas para detectar a resistência a 12 agentes antimicrobianos. As resistências mais freqüentes foram para tetraciclina (92,2%), estreptomicina (90,4%), ácido nalidixico (88,3%), amicacina (86,5%) e cefalotina (84,8%). A resistência a múltiplas drogas foi encontrada em 152 cepas (65,8%).Universidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasUniversidade de São Paulo, Faculdade de Odontologia de Ribeirão PretoUniversidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasColégio Brasileiro de Patologia Animal - CBPAUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Rangel, PatriciaMarin, José Moacir2021-07-14T10:21:22Z2021-07-14T10:21:22Z2009-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article363-368application/pdfhttp://dx.doi.org/10.1590/S0100-736X2009000500001Pesquisa Veterinária Brasileira. Rio de Janeiro, RJ, Brazil: Colégio Brasileiro de Patologia Animal - CBPA, v. 29, n. 5, p. 363-368, 2009.0100-736X1678-5150http://hdl.handle.net/11449/21124110.1590/S0100-736X2009000500001S0100-736X2009000500001S0100-736X2009000500001.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinária Brasileirainfo:eu-repo/semantics/openAccess2023-12-27T06:20:11Zoai:repositorio.unesp.br:11449/211241Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-27T06:20:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Analysis of Escherichia coli isolated from bovine mastitic milk
Análise de Escherichia coli isolada de leite de vacas com mastite
title Analysis of Escherichia coli isolated from bovine mastitic milk
spellingShingle Analysis of Escherichia coli isolated from bovine mastitic milk
Rangel, Patricia
Escherichia coli
mastitis
antimicrobial agents
multidrug resistance
STEC
eae gene
Escherichia coli
mastite
agentes antimicrobianos
resistência a múltiplas drogas
STEC
gene eae
title_short Analysis of Escherichia coli isolated from bovine mastitic milk
title_full Analysis of Escherichia coli isolated from bovine mastitic milk
title_fullStr Analysis of Escherichia coli isolated from bovine mastitic milk
title_full_unstemmed Analysis of Escherichia coli isolated from bovine mastitic milk
title_sort Analysis of Escherichia coli isolated from bovine mastitic milk
author Rangel, Patricia
author_facet Rangel, Patricia
Marin, José Moacir
author_role author
author2 Marin, José Moacir
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Rangel, Patricia
Marin, José Moacir
dc.subject.por.fl_str_mv Escherichia coli
mastitis
antimicrobial agents
multidrug resistance
STEC
eae gene
Escherichia coli
mastite
agentes antimicrobianos
resistência a múltiplas drogas
STEC
gene eae
topic Escherichia coli
mastitis
antimicrobial agents
multidrug resistance
STEC
eae gene
Escherichia coli
mastite
agentes antimicrobianos
resistência a múltiplas drogas
STEC
gene eae
description Mastitis has been recognized for some time as the most costly disease in dairy herds. From February to November 2004, 670 samples of bovine mastitic milk from which 231 Escherichia coli strains were isolated, were collected from two Brazilian states. The strains were screened for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twenty (8.6%) strains were detected by PCR to harbor the Shiga toxin genes (8 the stx 1 gene, 12 the stx 2 gene and none both of them). Two (0.8%) of the Escherichia coli strains studied were eae positive non Shiga toxin-producing. The strains were also examined for resistance to 12 antimicrobial agents. The predominantly observed resistance was to tetracycline (92.2%), streptomycin (90.4%), nalidixic acid (88.3%), amikacin (86.5%) and cephalothin (84.8%). Multidrug resistance was found among 152 isolates (65.8%).
publishDate 2009
dc.date.none.fl_str_mv 2009-05
2021-07-14T10:21:22Z
2021-07-14T10:21:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2009000500001
Pesquisa Veterinária Brasileira. Rio de Janeiro, RJ, Brazil: Colégio Brasileiro de Patologia Animal - CBPA, v. 29, n. 5, p. 363-368, 2009.
0100-736X
1678-5150
http://hdl.handle.net/11449/211241
10.1590/S0100-736X2009000500001
S0100-736X2009000500001
S0100-736X2009000500001.pdf
url http://dx.doi.org/10.1590/S0100-736X2009000500001
http://hdl.handle.net/11449/211241
identifier_str_mv Pesquisa Veterinária Brasileira. Rio de Janeiro, RJ, Brazil: Colégio Brasileiro de Patologia Animal - CBPA, v. 29, n. 5, p. 363-368, 2009.
0100-736X
1678-5150
10.1590/S0100-736X2009000500001
S0100-736X2009000500001
S0100-736X2009000500001.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinária Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 363-368
application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803047137034895360

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