In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://go.galegroup.com/ps/i.do?id=GALE%7CA317779721&v=2.1&u=capes58&it=r&p=AONE&sw=w http://hdl.handle.net/11449/11906 |
Resumo: | Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients.Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-gamma, IL-10, TGF-beta 1 and TNF-alpha were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-beta 1 were detected by immunohistochemistry in skin biopsies.Results: BT patients produced higher levels of IFN-gamma than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-beta 1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-beta 1 expression in skin biopsies, positive correlation between TGF-beta 1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN-gamma production.Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. |
id |
UNSP_9028d63011488febe56cfec51a87ecf6 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/11906 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosyObjective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients.Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-gamma, IL-10, TGF-beta 1 and TNF-alpha were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-beta 1 were detected by immunohistochemistry in skin biopsies.Results: BT patients produced higher levels of IFN-gamma than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-beta 1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-beta 1 expression in skin biopsies, positive correlation between TGF-beta 1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN-gamma production.Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Inst Lauro de Souza Lima, Equipe Tecn Biol, BR-17034971 Bauru, SP, BrazilUNESP, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, BrazilInst Lauro de Souza Lima, Equipe Tecn Patol, BR-17034971 Bauru, SP, BrazilInst Lauro de Souza Lima, Serv Epidemiol, BR-17034971 Bauru, SP, BrazilInst Lauro de Souza Lima, Div Pesquisa & Ensino, BR-17034971 Bauru, SP, BrazilUniv São Paulo, Fac Odontol Bauru, Bauru, SP, BrazilUNESP, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, BrazilLepraUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Venturini, James [UNESP]Soares, Cleverson TeixeiraFernandes Belone, Andrea de FariaBarreto, Jaison AntonioUra, SomeiPereira Lauris, Jose RobertoVilani-Moreno, Fatima Regina2014-05-20T13:34:41Z2014-05-20T13:34:41Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25-35http://go.galegroup.com/ps/i.do?id=GALE%7CA317779721&v=2.1&u=capes58&it=r&p=AONE&sw=wLeprosy Review. Colchester: Lepra, v. 82, n. 1, p. 25-35, 2011.0305-7518http://hdl.handle.net/11449/11906WOS:000298223600005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLeprosy Review0.7520,563info:eu-repo/semantics/openAccess2021-10-23T12:10:47Zoai:repositorio.unesp.br:11449/11906Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:10:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
spellingShingle |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy Venturini, James [UNESP] |
title_short |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_full |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_fullStr |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_full_unstemmed |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_sort |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
author |
Venturini, James [UNESP] |
author_facet |
Venturini, James [UNESP] Soares, Cleverson Teixeira Fernandes Belone, Andrea de Faria Barreto, Jaison Antonio Ura, Somei Pereira Lauris, Jose Roberto Vilani-Moreno, Fatima Regina |
author_role |
author |
author2 |
Soares, Cleverson Teixeira Fernandes Belone, Andrea de Faria Barreto, Jaison Antonio Ura, Somei Pereira Lauris, Jose Roberto Vilani-Moreno, Fatima Regina |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Venturini, James [UNESP] Soares, Cleverson Teixeira Fernandes Belone, Andrea de Faria Barreto, Jaison Antonio Ura, Somei Pereira Lauris, Jose Roberto Vilani-Moreno, Fatima Regina |
description |
Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients.Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-gamma, IL-10, TGF-beta 1 and TNF-alpha were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-beta 1 were detected by immunohistochemistry in skin biopsies.Results: BT patients produced higher levels of IFN-gamma than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-beta 1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-beta 1 expression in skin biopsies, positive correlation between TGF-beta 1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN-gamma production.Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2014-05-20T13:34:41Z 2014-05-20T13:34:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://go.galegroup.com/ps/i.do?id=GALE%7CA317779721&v=2.1&u=capes58&it=r&p=AONE&sw=w Leprosy Review. Colchester: Lepra, v. 82, n. 1, p. 25-35, 2011. 0305-7518 http://hdl.handle.net/11449/11906 WOS:000298223600005 |
url |
http://go.galegroup.com/ps/i.do?id=GALE%7CA317779721&v=2.1&u=capes58&it=r&p=AONE&sw=w http://hdl.handle.net/11449/11906 |
identifier_str_mv |
Leprosy Review. Colchester: Lepra, v. 82, n. 1, p. 25-35, 2011. 0305-7518 WOS:000298223600005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Leprosy Review 0.752 0,563 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25-35 |
dc.publisher.none.fl_str_mv |
Lepra |
publisher.none.fl_str_mv |
Lepra |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965756826320896 |