Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-09352012000300035 http://hdl.handle.net/11449/1682 |
Resumo: | Three selective enrichment broths - selenite cystine (SC), Muller-Kauffmann tetrathionate (MKT) and Rappaport-Vassiliadis (RV) - were compared, for Salmonella Typhimurium isolation from rectal swabs of a calf experimentally infected. The bacteriological procedure involved pre-enrichment in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment (SC, MKT and RV broths), culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antisera. SC and MKT broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological isolation (ten positive samples). |
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Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella TyphimuriumComparison of selenite cystine, tetrathionate Muller-Kauffmann and Rappaport-Vassiliadis broths for Salmonella Typhimurium isolationCalvesfecesselective enrichment brothSalmonella Typhimuriumbezerrofezesmeio de enriquecimento seletivoSalmonella TyphimuriumThree selective enrichment broths - selenite cystine (SC), Muller-Kauffmann tetrathionate (MKT) and Rappaport-Vassiliadis (RV) - were compared, for Salmonella Typhimurium isolation from rectal swabs of a calf experimentally infected. The bacteriological procedure involved pre-enrichment in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment (SC, MKT and RV broths), culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antisera. SC and MKT broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological isolation (ten positive samples).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Faculdade de Ciências Agrárias e VeterináriasUNESP Faculdade de Ciências Agrárias e VeterináriasUniversidade Federal de Minas Gerais (UFMG), Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Ávila, L.G.Silva, D.GFagliari, J.J. [UNESP]2014-05-20T13:14:07Z2014-05-20T13:14:07Z2012-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article773-775application/pdfhttp://dx.doi.org/10.1590/S0102-09352012000300035Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 64, n. 3, p. 773-775, 2012.0102-0935http://hdl.handle.net/11449/168210.1590/S0102-09352012000300035S0102-09352012000300035WOS:000306085200035S0102-09352012000300035.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-06-06T14:09:53Zoai:repositorio.unesp.br:11449/1682Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:02:17.753571Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium Comparison of selenite cystine, tetrathionate Muller-Kauffmann and Rappaport-Vassiliadis broths for Salmonella Typhimurium isolation |
title |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium |
spellingShingle |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium Ávila, L.G. Calves feces selective enrichment broth Salmonella Typhimurium bezerro fezes meio de enriquecimento seletivo Salmonella Typhimurium |
title_short |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium |
title_full |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium |
title_fullStr |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium |
title_full_unstemmed |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium |
title_sort |
Comparação dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium |
author |
Ávila, L.G. |
author_facet |
Ávila, L.G. Silva, D.G Fagliari, J.J. [UNESP] |
author_role |
author |
author2 |
Silva, D.G Fagliari, J.J. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ávila, L.G. Silva, D.G Fagliari, J.J. [UNESP] |
dc.subject.por.fl_str_mv |
Calves feces selective enrichment broth Salmonella Typhimurium bezerro fezes meio de enriquecimento seletivo Salmonella Typhimurium |
topic |
Calves feces selective enrichment broth Salmonella Typhimurium bezerro fezes meio de enriquecimento seletivo Salmonella Typhimurium |
description |
Three selective enrichment broths - selenite cystine (SC), Muller-Kauffmann tetrathionate (MKT) and Rappaport-Vassiliadis (RV) - were compared, for Salmonella Typhimurium isolation from rectal swabs of a calf experimentally infected. The bacteriological procedure involved pre-enrichment in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment (SC, MKT and RV broths), culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antisera. SC and MKT broths were more efficient in the isolation of Salmonella Typhimurium (12 positive samples), whereas RV broth had a lower efficiency in the microbiological isolation (ten positive samples). |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 2014-05-20T13:14:07Z 2014-05-20T13:14:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-09352012000300035 Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 64, n. 3, p. 773-775, 2012. 0102-0935 http://hdl.handle.net/11449/1682 10.1590/S0102-09352012000300035 S0102-09352012000300035 WOS:000306085200035 S0102-09352012000300035.pdf |
url |
http://dx.doi.org/10.1590/S0102-09352012000300035 http://hdl.handle.net/11449/1682 |
identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 64, n. 3, p. 773-775, 2012. 0102-0935 10.1590/S0102-09352012000300035 S0102-09352012000300035 WOS:000306085200035 S0102-09352012000300035.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia 0.286 0,248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
773-775 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128743719305216 |