Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats

Detalhes bibliográficos
Autor(a) principal: Beltrame, Flavia L.
Data de Publicação: 2012
Outros Autores: Yamauti, Caroline T., Caneguim, Breno H., Cerri, Paulo S., Miraglia, Sandra M., Sasso-Cerri, Estela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/196013
Resumo: Cimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature.
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spelling Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult ratsH-2 receptor antagonistMicrovascular densityVascular atrophyCell deathSmooth muscle cellCimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent,Fac Odontol Araraquara UNESP FOAr, BR-14801903 Araraquara, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, BrazilSao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent,Fac Odontol Araraquara UNESP FOAr, BR-14801903 Araraquara, SP, BrazilFAPESP: 2006/50324-3FAPESP: 2006/54776-6FAPESP: 2007/53207-0FAPESP: 2010/02409-5F HernandezUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Beltrame, Flavia L.Yamauti, Caroline T.Caneguim, Breno H.Cerri, Paulo S.Miraglia, Sandra M.Sasso-Cerri, Estela [UNESP]2020-12-10T19:30:31Z2020-12-10T19:30:31Z2012-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1343-1351Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012.0213-3911http://hdl.handle.net/11449/196013WOS:000308583700011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology And Histopathologyinfo:eu-repo/semantics/openAccess2024-09-27T15:15:10Zoai:repositorio.unesp.br:11449/196013Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
title Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
spellingShingle Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
Beltrame, Flavia L.
H-2 receptor antagonist
Microvascular density
Vascular atrophy
Cell death
Smooth muscle cell
title_short Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
title_full Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
title_fullStr Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
title_full_unstemmed Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
title_sort Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
author Beltrame, Flavia L.
author_facet Beltrame, Flavia L.
Yamauti, Caroline T.
Caneguim, Breno H.
Cerri, Paulo S.
Miraglia, Sandra M.
Sasso-Cerri, Estela [UNESP]
author_role author
author2 Yamauti, Caroline T.
Caneguim, Breno H.
Cerri, Paulo S.
Miraglia, Sandra M.
Sasso-Cerri, Estela [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Beltrame, Flavia L.
Yamauti, Caroline T.
Caneguim, Breno H.
Cerri, Paulo S.
Miraglia, Sandra M.
Sasso-Cerri, Estela [UNESP]
dc.subject.por.fl_str_mv H-2 receptor antagonist
Microvascular density
Vascular atrophy
Cell death
Smooth muscle cell
topic H-2 receptor antagonist
Microvascular density
Vascular atrophy
Cell death
Smooth muscle cell
description Cimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01
2020-12-10T19:30:31Z
2020-12-10T19:30:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012.
0213-3911
http://hdl.handle.net/11449/196013
WOS:000308583700011
identifier_str_mv Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012.
0213-3911
WOS:000308583700011
url http://hdl.handle.net/11449/196013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Histology And Histopathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1343-1351
dc.publisher.none.fl_str_mv F Hernandez
publisher.none.fl_str_mv F Hernandez
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1813546487906304000