Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/196013 |
Resumo: | Cimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature. |
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Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult ratsH-2 receptor antagonistMicrovascular densityVascular atrophyCell deathSmooth muscle cellCimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent,Fac Odontol Araraquara UNESP FOAr, BR-14801903 Araraquara, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Sao Paulo, BrazilSao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent,Fac Odontol Araraquara UNESP FOAr, BR-14801903 Araraquara, SP, BrazilFAPESP: 2006/50324-3FAPESP: 2006/54776-6FAPESP: 2007/53207-0FAPESP: 2010/02409-5F HernandezUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Beltrame, Flavia L.Yamauti, Caroline T.Caneguim, Breno H.Cerri, Paulo S.Miraglia, Sandra M.Sasso-Cerri, Estela [UNESP]2020-12-10T19:30:31Z2020-12-10T19:30:31Z2012-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1343-1351Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012.0213-3911http://hdl.handle.net/11449/196013WOS:000308583700011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology And Histopathologyinfo:eu-repo/semantics/openAccess2024-09-27T15:15:10Zoai:repositorio.unesp.br:11449/196013Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:15:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
title |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
spellingShingle |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats Beltrame, Flavia L. H-2 receptor antagonist Microvascular density Vascular atrophy Cell death Smooth muscle cell |
title_short |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
title_full |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
title_fullStr |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
title_full_unstemmed |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
title_sort |
Cimetidine-induced vascular cell apoptosis impairs testicular microvasculature in adult rats |
author |
Beltrame, Flavia L. |
author_facet |
Beltrame, Flavia L. Yamauti, Caroline T. Caneguim, Breno H. Cerri, Paulo S. Miraglia, Sandra M. Sasso-Cerri, Estela [UNESP] |
author_role |
author |
author2 |
Yamauti, Caroline T. Caneguim, Breno H. Cerri, Paulo S. Miraglia, Sandra M. Sasso-Cerri, Estela [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Beltrame, Flavia L. Yamauti, Caroline T. Caneguim, Breno H. Cerri, Paulo S. Miraglia, Sandra M. Sasso-Cerri, Estela [UNESP] |
dc.subject.por.fl_str_mv |
H-2 receptor antagonist Microvascular density Vascular atrophy Cell death Smooth muscle cell |
topic |
H-2 receptor antagonist Microvascular density Vascular atrophy Cell death Smooth muscle cell |
description |
Cimetidine, an H-2 receptor antagonist used for treatment of gastric ulcers, exerts antiandrogenic and antiangiogenic effects. In the testes cimetidine impairs spermatogenesis, Sertoli cells and peritubular tissue, inducing apoptosis in the myoid cells. Regarding the importance of histamine and androgens for vascular maintenance, the effect of cimetidine on the structural integrity of the testicular vasculature was evaluated. Adult male rats received cimetidine (CMTG) and saline (CG) for 50 days. The testes were fixed in buffered 4% formaldehyde and embedded in historesin and paraffin. In the PAS-stained sections, the microvascular density (MVD) and the vascular luminal area (VLA) were obtained. TUNEL method was performed for detection of cell death. Testicular fragments embedded in Araldite were analyzed under transmission electron microscopy. A significant decrease in the MVD and VLA and a high number of collapsed blood vessel profiles were observed in CMTG. Endothelial cells and vascular muscle cells were TUNEL-positive and showed ultrastructural features of apoptosis. These results indicate that cimetidine induces apoptosis in vascular cells, leading to testicular vascular atrophy. A possible antagonist effect of cimetidine on the H-2 receptors and/or androgen receptors in the vascular cells may be responsible for the impairment of the testicular microvasculature. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 2020-12-10T19:30:31Z 2020-12-10T19:30:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012. 0213-3911 http://hdl.handle.net/11449/196013 WOS:000308583700011 |
identifier_str_mv |
Histology And Histopathology. Murcia: F Hernandez, v. 27, n. 10, p. 1343-1351, 2012. 0213-3911 WOS:000308583700011 |
url |
http://hdl.handle.net/11449/196013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Histology And Histopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1343-1351 |
dc.publisher.none.fl_str_mv |
F Hernandez |
publisher.none.fl_str_mv |
F Hernandez |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546487906304000 |