Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2020.101815 http://hdl.handle.net/11449/198927 |
Resumo: | Photodynamic therapy has been applied for the treatment of many diseases, especially skin diseases. However, poor aqueous solubility and toxicity of some photosensitizer drugs are the main disadvantages for their direct clinical applications. Thus, biotechnology and nanotechnology are important tools in the development of new ways of obtaining photoactive compounds that are biocompatible. We investigated the potential of a new nanostructured photosensitizer, an anthraquinone derivative produced by biotechnological process; then we associated nanotechnology to obtain a nanostructured anthraquinone active molecule. For this, it was prepared a classical nanocapsule formulations containing poly(lactide-co-glycolide) (PLGA) coating for encapsulation of anthraquinone derivative. These formulations were characterized by their physicochemical, morphological, photophysical properties, and stability. We performed in vitro biocompatibility and photodynamic activity assays of free and nanostructured anthraquinone. Nanocapsule formulations containing anthraquinone derivative showed a nanometric profile with particle size around 250 nm, negative zeta potential around −30 mV, and partially monodisperse. Besides that, characteristic spherical morphology of nanocapsules and homogeneous particle surface were observed by AFM analyses. The in vitro biocompatibility assay showed absence of cytotoxicity for all tested RD/NC concentrations and also for unloaded/NC in NIH3T3 cells. In vitro photoactivation assay using NIH3T3 cells showed that nanocapsules promoted greater drug uptake by NIH3T3 cells, around of 87%, of cell death compared to free drug showed around 48% of cell death. The anthraquinone derivative showed potential for use in PDT. Besides the association with nanocapsules improved cell uptake of photosensitizer resulting in increased cell death compared to free anthraquinone. |
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Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapyAnthraquinoneBiotechnological photosensitizersPhotodynamic therapyPLGA-nanocapsulePolymeric nanomaterialsPhotodynamic therapy has been applied for the treatment of many diseases, especially skin diseases. However, poor aqueous solubility and toxicity of some photosensitizer drugs are the main disadvantages for their direct clinical applications. Thus, biotechnology and nanotechnology are important tools in the development of new ways of obtaining photoactive compounds that are biocompatible. We investigated the potential of a new nanostructured photosensitizer, an anthraquinone derivative produced by biotechnological process; then we associated nanotechnology to obtain a nanostructured anthraquinone active molecule. For this, it was prepared a classical nanocapsule formulations containing poly(lactide-co-glycolide) (PLGA) coating for encapsulation of anthraquinone derivative. These formulations were characterized by their physicochemical, morphological, photophysical properties, and stability. We performed in vitro biocompatibility and photodynamic activity assays of free and nanostructured anthraquinone. Nanocapsule formulations containing anthraquinone derivative showed a nanometric profile with particle size around 250 nm, negative zeta potential around −30 mV, and partially monodisperse. Besides that, characteristic spherical morphology of nanocapsules and homogeneous particle surface were observed by AFM analyses. The in vitro biocompatibility assay showed absence of cytotoxicity for all tested RD/NC concentrations and also for unloaded/NC in NIH3T3 cells. In vitro photoactivation assay using NIH3T3 cells showed that nanocapsules promoted greater drug uptake by NIH3T3 cells, around of 87%, of cell death compared to free drug showed around 48% of cell death. The anthraquinone derivative showed potential for use in PDT. Besides the association with nanocapsules improved cell uptake of photosensitizer resulting in increased cell death compared to free anthraquinone.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University - UNESPDepartment of Chemistry Center of Nanotechnology and Tissue Engineering – Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo - USPGraduate Program of Chemical Engineering Federal University of São CarlosCollege of Pharmaceutical Sciences Federal University of Sao Francisco Valley - UNIVASFDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University - UNESPUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Federal University of Sao Francisco Valley - UNIVASFAmantino, Camila F. [UNESP]de Baptista-Neto, Álvaro [UNESP]Badino, Alberto C.Siqueira-Moura, Marigilson P.Tedesco, Antonio C.Primo, Fernando L. [UNESP]2020-12-12T01:25:44Z2020-12-12T01:25:44Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2020.101815Photodiagnosis and Photodynamic Therapy, v. 31.1873-15971572-1000http://hdl.handle.net/11449/19892710.1016/j.pdpdt.2020.1018152-s2.0-85085888786Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2021-10-22T21:03:08Zoai:repositorio.unesp.br:11449/198927Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:11:39.201522Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
title |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
spellingShingle |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy Amantino, Camila F. [UNESP] Anthraquinone Biotechnological photosensitizers Photodynamic therapy PLGA-nanocapsule Polymeric nanomaterials |
title_short |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
title_full |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
title_fullStr |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
title_full_unstemmed |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
title_sort |
Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy |
author |
Amantino, Camila F. [UNESP] |
author_facet |
Amantino, Camila F. [UNESP] de Baptista-Neto, Álvaro [UNESP] Badino, Alberto C. Siqueira-Moura, Marigilson P. Tedesco, Antonio C. Primo, Fernando L. [UNESP] |
author_role |
author |
author2 |
de Baptista-Neto, Álvaro [UNESP] Badino, Alberto C. Siqueira-Moura, Marigilson P. Tedesco, Antonio C. Primo, Fernando L. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) Federal University of Sao Francisco Valley - UNIVASF |
dc.contributor.author.fl_str_mv |
Amantino, Camila F. [UNESP] de Baptista-Neto, Álvaro [UNESP] Badino, Alberto C. Siqueira-Moura, Marigilson P. Tedesco, Antonio C. Primo, Fernando L. [UNESP] |
dc.subject.por.fl_str_mv |
Anthraquinone Biotechnological photosensitizers Photodynamic therapy PLGA-nanocapsule Polymeric nanomaterials |
topic |
Anthraquinone Biotechnological photosensitizers Photodynamic therapy PLGA-nanocapsule Polymeric nanomaterials |
description |
Photodynamic therapy has been applied for the treatment of many diseases, especially skin diseases. However, poor aqueous solubility and toxicity of some photosensitizer drugs are the main disadvantages for their direct clinical applications. Thus, biotechnology and nanotechnology are important tools in the development of new ways of obtaining photoactive compounds that are biocompatible. We investigated the potential of a new nanostructured photosensitizer, an anthraquinone derivative produced by biotechnological process; then we associated nanotechnology to obtain a nanostructured anthraquinone active molecule. For this, it was prepared a classical nanocapsule formulations containing poly(lactide-co-glycolide) (PLGA) coating for encapsulation of anthraquinone derivative. These formulations were characterized by their physicochemical, morphological, photophysical properties, and stability. We performed in vitro biocompatibility and photodynamic activity assays of free and nanostructured anthraquinone. Nanocapsule formulations containing anthraquinone derivative showed a nanometric profile with particle size around 250 nm, negative zeta potential around −30 mV, and partially monodisperse. Besides that, characteristic spherical morphology of nanocapsules and homogeneous particle surface were observed by AFM analyses. The in vitro biocompatibility assay showed absence of cytotoxicity for all tested RD/NC concentrations and also for unloaded/NC in NIH3T3 cells. In vitro photoactivation assay using NIH3T3 cells showed that nanocapsules promoted greater drug uptake by NIH3T3 cells, around of 87%, of cell death compared to free drug showed around 48% of cell death. The anthraquinone derivative showed potential for use in PDT. Besides the association with nanocapsules improved cell uptake of photosensitizer resulting in increased cell death compared to free anthraquinone. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:25:44Z 2020-12-12T01:25:44Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2020.101815 Photodiagnosis and Photodynamic Therapy, v. 31. 1873-1597 1572-1000 http://hdl.handle.net/11449/198927 10.1016/j.pdpdt.2020.101815 2-s2.0-85085888786 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2020.101815 http://hdl.handle.net/11449/198927 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 31. 1873-1597 1572-1000 10.1016/j.pdpdt.2020.101815 2-s2.0-85085888786 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129593872220160 |