Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells

Detalhes bibliográficos
Autor(a) principal: Balera Brito, Victor Gustavo
Data de Publicação: 2019
Outros Autores: Chaves-Neto, Antonio Hernandes, Landim de Barros, Thamine, Penha Oliveira, Sandra Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jep.2019.112131
http://hdl.handle.net/11449/189730
Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro.
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spelling Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cellsBone marrow-derived mesenchymal stromal cellsIlex paraguariensisOsteoblast differentiationSoluble yerba mateETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro.Programa Multicêntrico de Pós-Graduaçãoem Ciências Fisiológicas, SBFis/UNESP, Brazil; Laboratory of Pharmacology, Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil. Electronic address: victorbalera@gmail.comPrograma Multicêntrico de Pós-Graduaçãoem Ciências Fisiológicas, SBFis/UNESP, Brazil; Laboratory of Biochemistry, Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, BrazilPrograma Multicêntrico de Pós-Graduaçãoem Ciências Fisiológicas, SBFis/UNESP, Brazil; Laboratory of Pharmacology, Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, BrazilUniversidade Estadual Paulista (Unesp)Balera Brito, Victor GustavoChaves-Neto, Antonio HernandesLandim de Barros, ThaminePenha Oliveira, Sandra Helena2019-10-06T16:50:23Z2019-10-06T16:50:23Z2019-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article112131http://dx.doi.org/10.1016/j.jep.2019.112131Journal of ethnopharmacology, v. 244, p. 112131-.1872-7573http://hdl.handle.net/11449/18973010.1016/j.jep.2019.1121312-s2.0-85071782540Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of ethnopharmacologyinfo:eu-repo/semantics/openAccess2024-04-10T14:46:00Zoai:repositorio.unesp.br:11449/189730Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:52:22.353459Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
title Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
spellingShingle Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
Balera Brito, Victor Gustavo
Bone marrow-derived mesenchymal stromal cells
Ilex paraguariensis
Osteoblast differentiation
Soluble yerba mate
title_short Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
title_full Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
title_fullStr Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
title_full_unstemmed Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
title_sort Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
author Balera Brito, Victor Gustavo
author_facet Balera Brito, Victor Gustavo
Chaves-Neto, Antonio Hernandes
Landim de Barros, Thamine
Penha Oliveira, Sandra Helena
author_role author
author2 Chaves-Neto, Antonio Hernandes
Landim de Barros, Thamine
Penha Oliveira, Sandra Helena
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Balera Brito, Victor Gustavo
Chaves-Neto, Antonio Hernandes
Landim de Barros, Thamine
Penha Oliveira, Sandra Helena
dc.subject.por.fl_str_mv Bone marrow-derived mesenchymal stromal cells
Ilex paraguariensis
Osteoblast differentiation
Soluble yerba mate
topic Bone marrow-derived mesenchymal stromal cells
Ilex paraguariensis
Osteoblast differentiation
Soluble yerba mate
description ETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:50:23Z
2019-10-06T16:50:23Z
2019-11-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jep.2019.112131
Journal of ethnopharmacology, v. 244, p. 112131-.
1872-7573
http://hdl.handle.net/11449/189730
10.1016/j.jep.2019.112131
2-s2.0-85071782540
url http://dx.doi.org/10.1016/j.jep.2019.112131
http://hdl.handle.net/11449/189730
identifier_str_mv Journal of ethnopharmacology, v. 244, p. 112131-.
1872-7573
10.1016/j.jep.2019.112131
2-s2.0-85071782540
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of ethnopharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 112131
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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