Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2019.112131 http://hdl.handle.net/11449/189730 |
Resumo: | ETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro. |
id |
UNSP_910d70298cb3b1512acb376dfe47613a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/189730 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cellsBone marrow-derived mesenchymal stromal cellsIlex paraguariensisOsteoblast differentiationSoluble yerba mateETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro.Programa Multicêntrico de Pós-Graduaçãoem Ciências Fisiológicas, SBFis/UNESP, Brazil; Laboratory of Pharmacology, Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil. Electronic address: victorbalera@gmail.comPrograma Multicêntrico de Pós-Graduaçãoem Ciências Fisiológicas, SBFis/UNESP, Brazil; Laboratory of Biochemistry, Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, BrazilPrograma Multicêntrico de Pós-Graduaçãoem Ciências Fisiológicas, SBFis/UNESP, Brazil; Laboratory of Pharmacology, Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, BrazilUniversidade Estadual Paulista (Unesp)Balera Brito, Victor GustavoChaves-Neto, Antonio HernandesLandim de Barros, ThaminePenha Oliveira, Sandra Helena2019-10-06T16:50:23Z2019-10-06T16:50:23Z2019-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article112131http://dx.doi.org/10.1016/j.jep.2019.112131Journal of ethnopharmacology, v. 244, p. 112131-.1872-7573http://hdl.handle.net/11449/18973010.1016/j.jep.2019.1121312-s2.0-85071782540Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of ethnopharmacologyinfo:eu-repo/semantics/openAccess2024-04-10T14:46:00Zoai:repositorio.unesp.br:11449/189730Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:52:22.353459Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
title |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
spellingShingle |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells Balera Brito, Victor Gustavo Bone marrow-derived mesenchymal stromal cells Ilex paraguariensis Osteoblast differentiation Soluble yerba mate |
title_short |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
title_full |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
title_fullStr |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
title_full_unstemmed |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
title_sort |
Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells |
author |
Balera Brito, Victor Gustavo |
author_facet |
Balera Brito, Victor Gustavo Chaves-Neto, Antonio Hernandes Landim de Barros, Thamine Penha Oliveira, Sandra Helena |
author_role |
author |
author2 |
Chaves-Neto, Antonio Hernandes Landim de Barros, Thamine Penha Oliveira, Sandra Helena |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Balera Brito, Victor Gustavo Chaves-Neto, Antonio Hernandes Landim de Barros, Thamine Penha Oliveira, Sandra Helena |
dc.subject.por.fl_str_mv |
Bone marrow-derived mesenchymal stromal cells Ilex paraguariensis Osteoblast differentiation Soluble yerba mate |
topic |
Bone marrow-derived mesenchymal stromal cells Ilex paraguariensis Osteoblast differentiation Soluble yerba mate |
description |
ETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:50:23Z 2019-10-06T16:50:23Z 2019-11-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2019.112131 Journal of ethnopharmacology, v. 244, p. 112131-. 1872-7573 http://hdl.handle.net/11449/189730 10.1016/j.jep.2019.112131 2-s2.0-85071782540 |
url |
http://dx.doi.org/10.1016/j.jep.2019.112131 http://hdl.handle.net/11449/189730 |
identifier_str_mv |
Journal of ethnopharmacology, v. 244, p. 112131-. 1872-7573 10.1016/j.jep.2019.112131 2-s2.0-85071782540 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of ethnopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
112131 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129368091787264 |