Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/jpn.13478 http://hdl.handle.net/11449/210615 |
Resumo: | Most amino acid requirement trials appear for whole-body responses, but there is little information concerning amino acid incorporation in individual tissues, which may vary according to the age. L-[N-15] threonine was used to evaluate its incorporation rate and distribution among broiler tissues in different ages. Seventy-two male broiler chickens were distributed into three different phases: starter (4 to 9 days old), grower (18 to 23 days old) and finisher phase (32 to 37 days old). L-[N-15] threonine was added on balanced diets, and birds were fed for five days in each phase. Enriched samples of breast muscle, feathers, liver, jejunum and plasma were collected at 0, 6, 12, 24, 48, 72, 96 and 120 hr after fed birds with the tracer in each phase. In the tissues were analysed dry matter, nitrogen and stable nitrogen. The N-15 isotope abundance according to the time was fitted into exponential or linear equations using a same intercept. The ratio of the steepness or slope coefficients was determined to compare the L-[N-15] threonine incorporation according to the age. In addition, L-[N-15] threonine mass balances were performed to assess the L-[N-15] threonine distribution among the evaluated tissues. Except for feathers, the L-[N-15] threonine incorporation rate decreased with ageing. Taking into account the L-[N-15] threonine distribution in the tissues, only in the jejunum was not observed an increase as the broiler grew. The L-[N-15] incorporation varied in each tissue and according to the age of the broiler chickens. These outcomes could be useful to comprehend changes in amino acid requirements tissue-specific according to age. |
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Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonineamino acidisotopemetabolismnitrogenproteinMost amino acid requirement trials appear for whole-body responses, but there is little information concerning amino acid incorporation in individual tissues, which may vary according to the age. L-[N-15] threonine was used to evaluate its incorporation rate and distribution among broiler tissues in different ages. Seventy-two male broiler chickens were distributed into three different phases: starter (4 to 9 days old), grower (18 to 23 days old) and finisher phase (32 to 37 days old). L-[N-15] threonine was added on balanced diets, and birds were fed for five days in each phase. Enriched samples of breast muscle, feathers, liver, jejunum and plasma were collected at 0, 6, 12, 24, 48, 72, 96 and 120 hr after fed birds with the tracer in each phase. In the tissues were analysed dry matter, nitrogen and stable nitrogen. The N-15 isotope abundance according to the time was fitted into exponential or linear equations using a same intercept. The ratio of the steepness or slope coefficients was determined to compare the L-[N-15] threonine incorporation according to the age. In addition, L-[N-15] threonine mass balances were performed to assess the L-[N-15] threonine distribution among the evaluated tissues. Except for feathers, the L-[N-15] threonine incorporation rate decreased with ageing. Taking into account the L-[N-15] threonine distribution in the tissues, only in the jejunum was not observed an increase as the broiler grew. The L-[N-15] incorporation varied in each tissue and according to the age of the broiler chickens. These outcomes could be useful to comprehend changes in amino acid requirements tissue-specific according to age.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Sch Agr & Veterinarian Sci, Jaboticabal, BrazilSao Paulo State Univ, Stable Isotope Ctr Prof Carlos Ducatti, Botucatu, SP, BrazilSao Paulo State Univ, Sch Agr & Veterinarian Sci, Jaboticabal, BrazilSao Paulo State Univ, Stable Isotope Ctr Prof Carlos Ducatti, Botucatu, SP, BrazilFAPESP: 2013/25761-4CAPES: 1318250Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Suzuki, Rafael Massami [UNESP]Reis, Matheus de Paula [UNESP]Denadai, Juliana Celia [UNESP]Pacheco, Leticia Graziele [UNESP]Nagib Nascimento, Carolina Cardoso [UNESP]Sakomura, Nilva Kazue [UNESP]2021-06-25T23:35:38Z2021-06-25T23:35:38Z2021-01-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5http://dx.doi.org/10.1111/jpn.13478Journal Of Animal Physiology And Animal Nutrition. Hoboken: Wiley, 5 p., 2021.0931-2439http://hdl.handle.net/11449/21061510.1111/jpn.13478WOS:000605310500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Animal Physiology And Animal Nutritioninfo:eu-repo/semantics/openAccess2024-06-07T18:42:05Zoai:repositorio.unesp.br:11449/210615Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T18:42:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
title |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
spellingShingle |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine Suzuki, Rafael Massami [UNESP] amino acid isotope metabolism nitrogen protein |
title_short |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
title_full |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
title_fullStr |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
title_full_unstemmed |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
title_sort |
Threonine incorporation in tissues of growing broiler chickens using L-[N-15] threonine |
author |
Suzuki, Rafael Massami [UNESP] |
author_facet |
Suzuki, Rafael Massami [UNESP] Reis, Matheus de Paula [UNESP] Denadai, Juliana Celia [UNESP] Pacheco, Leticia Graziele [UNESP] Nagib Nascimento, Carolina Cardoso [UNESP] Sakomura, Nilva Kazue [UNESP] |
author_role |
author |
author2 |
Reis, Matheus de Paula [UNESP] Denadai, Juliana Celia [UNESP] Pacheco, Leticia Graziele [UNESP] Nagib Nascimento, Carolina Cardoso [UNESP] Sakomura, Nilva Kazue [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Suzuki, Rafael Massami [UNESP] Reis, Matheus de Paula [UNESP] Denadai, Juliana Celia [UNESP] Pacheco, Leticia Graziele [UNESP] Nagib Nascimento, Carolina Cardoso [UNESP] Sakomura, Nilva Kazue [UNESP] |
dc.subject.por.fl_str_mv |
amino acid isotope metabolism nitrogen protein |
topic |
amino acid isotope metabolism nitrogen protein |
description |
Most amino acid requirement trials appear for whole-body responses, but there is little information concerning amino acid incorporation in individual tissues, which may vary according to the age. L-[N-15] threonine was used to evaluate its incorporation rate and distribution among broiler tissues in different ages. Seventy-two male broiler chickens were distributed into three different phases: starter (4 to 9 days old), grower (18 to 23 days old) and finisher phase (32 to 37 days old). L-[N-15] threonine was added on balanced diets, and birds were fed for five days in each phase. Enriched samples of breast muscle, feathers, liver, jejunum and plasma were collected at 0, 6, 12, 24, 48, 72, 96 and 120 hr after fed birds with the tracer in each phase. In the tissues were analysed dry matter, nitrogen and stable nitrogen. The N-15 isotope abundance according to the time was fitted into exponential or linear equations using a same intercept. The ratio of the steepness or slope coefficients was determined to compare the L-[N-15] threonine incorporation according to the age. In addition, L-[N-15] threonine mass balances were performed to assess the L-[N-15] threonine distribution among the evaluated tissues. Except for feathers, the L-[N-15] threonine incorporation rate decreased with ageing. Taking into account the L-[N-15] threonine distribution in the tissues, only in the jejunum was not observed an increase as the broiler grew. The L-[N-15] incorporation varied in each tissue and according to the age of the broiler chickens. These outcomes could be useful to comprehend changes in amino acid requirements tissue-specific according to age. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T23:35:38Z 2021-06-25T23:35:38Z 2021-01-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jpn.13478 Journal Of Animal Physiology And Animal Nutrition. Hoboken: Wiley, 5 p., 2021. 0931-2439 http://hdl.handle.net/11449/210615 10.1111/jpn.13478 WOS:000605310500001 |
url |
http://dx.doi.org/10.1111/jpn.13478 http://hdl.handle.net/11449/210615 |
identifier_str_mv |
Journal Of Animal Physiology And Animal Nutrition. Hoboken: Wiley, 5 p., 2021. 0931-2439 10.1111/jpn.13478 WOS:000605310500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Animal Physiology And Animal Nutrition |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045363328745472 |