Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/mdr.2019.0038 http://hdl.handle.net/11449/185854 |
Resumo: | We investigated the responses and mechanisms of action of methicillin-resistant Staphylococcus aureus (MRSA) metabolism when exposed under sublethal concentrations of the synergistic antibacterial combination of nisin + oxacillin (1/4 of maximum sublethal concentration) and sublethal concentrations of oxacillin only and nisin only. A total of 135 proteins were identified, showing an alteration in the expression of 85 proteins when treatment was compared with untreated bacteria (control). When the bacteria were treated using the combination, there was an increase in the expression of proteins related to resistance (e.g., beta-lactamase) and also in the ones involved in protein synthesis, and there was a decrease in the expression of proteins related to stress and alterations in proteins related to bacterial energy metabolism. Bacterial oxidative stress showed that the combination was able to induce oxidative stress (p < 0.05) and increase enzyme activities and lipid hydroperoxide levels compared with individual treatments. The analysis of cell ultrastructure showed damage in MRSA, especially on the bacterial wall and the plasma membrane, with cell lysis and death. Thus, the changes caused by these treatments affected different proteins related to the bacterial biological processes and signaling pathways such as cell division, structure, stress, regulation, bacterial resistance, protein synthesis, gene expression, energetic metabolism, and virulence. It was observed that synergism among antimicrobials has high potential in therapeutic use and may reduce the required amounts of antibacterial substances in addition to being effective on different targets in bacterial cells. |
id |
UNSP_91e5dc80890b3ffd7f1cbe48e54414a3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/185854 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillinantimicrobial peptidebacterial resistancesynergismLC-MS/MSoxidative stresstransmission electron microscopyWe investigated the responses and mechanisms of action of methicillin-resistant Staphylococcus aureus (MRSA) metabolism when exposed under sublethal concentrations of the synergistic antibacterial combination of nisin + oxacillin (1/4 of maximum sublethal concentration) and sublethal concentrations of oxacillin only and nisin only. A total of 135 proteins were identified, showing an alteration in the expression of 85 proteins when treatment was compared with untreated bacteria (control). When the bacteria were treated using the combination, there was an increase in the expression of proteins related to resistance (e.g., beta-lactamase) and also in the ones involved in protein synthesis, and there was a decrease in the expression of proteins related to stress and alterations in proteins related to bacterial energy metabolism. Bacterial oxidative stress showed that the combination was able to induce oxidative stress (p < 0.05) and increase enzyme activities and lipid hydroperoxide levels compared with individual treatments. The analysis of cell ultrastructure showed damage in MRSA, especially on the bacterial wall and the plasma membrane, with cell lysis and death. Thus, the changes caused by these treatments affected different proteins related to the bacterial biological processes and signaling pathways such as cell division, structure, stress, regulation, bacterial resistance, protein synthesis, gene expression, energetic metabolism, and virulence. It was observed that synergism among antimicrobials has high potential in therapeutic use and may reduce the required amounts of antibacterial substances in addition to being effective on different targets in bacterial cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ UNESP, IBB, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, BrazilSao Paulo State Univ UNESP, IBB, Dept Chem & Biochem, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch FMB, Grad Program Trop Dis, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Ctr Study Venom & Venomous Anim CEVAP, Botucatu, SP, BrazilUniv Paranaense UNIPAR, Grad Program Anim Sci Emphasis Bioact Prod, Umuarama, BrazilSao Paulo State Univ UNESP, IBB, Elect Microscopy Ctr, Botucatu, SP, BrazilSao Paulo State Univ UNESP, IBB, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, BrazilSao Paulo State Univ UNESP, IBB, Dept Chem & Biochem, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch FMB, Grad Program Trop Dis, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Ctr Study Venom & Venomous Anim CEVAP, Botucatu, SP, BrazilSao Paulo State Univ UNESP, IBB, Elect Microscopy Ctr, Botucatu, SP, BrazilFAPESP: 2015/14278-6Mary Ann Liebert, IncUniversidade Estadual Paulista (Unesp)Univ Paranaense UNIPARBergamo Alves, Fernanda Cristina [UNESP]Albano, Mariana [UNESP]Murbach Teles Andrade, Bruna Fernanda [UNESP]Chechi, Jessica Luana [UNESP]Marques Pereira, Ana Flavia [UNESP]Furlanetto, Alessandra [UNESP]Mores Rall, Vera Lucia [UNESP]Henrique Fernandes, Ana Angelica [UNESP]Santos, Lucilene Delazari dos [UNESP]Barbosa, Lidiane NunesFernandes Junior, Ary [UNESP]2019-10-04T12:39:10Z2019-10-04T12:39:10Z2019-06-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1089/mdr.2019.0038Microbial Drug Resistance. New Rochelle: Mary Ann Liebert, Inc, 11 p., 2019.1076-6294http://hdl.handle.net/11449/18585410.1089/mdr.2019.0038WOS:0004733051000013368404126695911Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Drug Resistanceinfo:eu-repo/semantics/openAccess2021-10-22T18:13:15Zoai:repositorio.unesp.br:11449/185854Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T18:13:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
title |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
spellingShingle |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin Bergamo Alves, Fernanda Cristina [UNESP] antimicrobial peptide bacterial resistance synergism LC-MS/MS oxidative stress transmission electron microscopy |
title_short |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
title_full |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
title_fullStr |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
title_full_unstemmed |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
title_sort |
Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin |
author |
Bergamo Alves, Fernanda Cristina [UNESP] |
author_facet |
Bergamo Alves, Fernanda Cristina [UNESP] Albano, Mariana [UNESP] Murbach Teles Andrade, Bruna Fernanda [UNESP] Chechi, Jessica Luana [UNESP] Marques Pereira, Ana Flavia [UNESP] Furlanetto, Alessandra [UNESP] Mores Rall, Vera Lucia [UNESP] Henrique Fernandes, Ana Angelica [UNESP] Santos, Lucilene Delazari dos [UNESP] Barbosa, Lidiane Nunes Fernandes Junior, Ary [UNESP] |
author_role |
author |
author2 |
Albano, Mariana [UNESP] Murbach Teles Andrade, Bruna Fernanda [UNESP] Chechi, Jessica Luana [UNESP] Marques Pereira, Ana Flavia [UNESP] Furlanetto, Alessandra [UNESP] Mores Rall, Vera Lucia [UNESP] Henrique Fernandes, Ana Angelica [UNESP] Santos, Lucilene Delazari dos [UNESP] Barbosa, Lidiane Nunes Fernandes Junior, Ary [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Paranaense UNIPAR |
dc.contributor.author.fl_str_mv |
Bergamo Alves, Fernanda Cristina [UNESP] Albano, Mariana [UNESP] Murbach Teles Andrade, Bruna Fernanda [UNESP] Chechi, Jessica Luana [UNESP] Marques Pereira, Ana Flavia [UNESP] Furlanetto, Alessandra [UNESP] Mores Rall, Vera Lucia [UNESP] Henrique Fernandes, Ana Angelica [UNESP] Santos, Lucilene Delazari dos [UNESP] Barbosa, Lidiane Nunes Fernandes Junior, Ary [UNESP] |
dc.subject.por.fl_str_mv |
antimicrobial peptide bacterial resistance synergism LC-MS/MS oxidative stress transmission electron microscopy |
topic |
antimicrobial peptide bacterial resistance synergism LC-MS/MS oxidative stress transmission electron microscopy |
description |
We investigated the responses and mechanisms of action of methicillin-resistant Staphylococcus aureus (MRSA) metabolism when exposed under sublethal concentrations of the synergistic antibacterial combination of nisin + oxacillin (1/4 of maximum sublethal concentration) and sublethal concentrations of oxacillin only and nisin only. A total of 135 proteins were identified, showing an alteration in the expression of 85 proteins when treatment was compared with untreated bacteria (control). When the bacteria were treated using the combination, there was an increase in the expression of proteins related to resistance (e.g., beta-lactamase) and also in the ones involved in protein synthesis, and there was a decrease in the expression of proteins related to stress and alterations in proteins related to bacterial energy metabolism. Bacterial oxidative stress showed that the combination was able to induce oxidative stress (p < 0.05) and increase enzyme activities and lipid hydroperoxide levels compared with individual treatments. The analysis of cell ultrastructure showed damage in MRSA, especially on the bacterial wall and the plasma membrane, with cell lysis and death. Thus, the changes caused by these treatments affected different proteins related to the bacterial biological processes and signaling pathways such as cell division, structure, stress, regulation, bacterial resistance, protein synthesis, gene expression, energetic metabolism, and virulence. It was observed that synergism among antimicrobials has high potential in therapeutic use and may reduce the required amounts of antibacterial substances in addition to being effective on different targets in bacterial cells. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:39:10Z 2019-10-04T12:39:10Z 2019-06-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/mdr.2019.0038 Microbial Drug Resistance. New Rochelle: Mary Ann Liebert, Inc, 11 p., 2019. 1076-6294 http://hdl.handle.net/11449/185854 10.1089/mdr.2019.0038 WOS:000473305100001 3368404126695911 |
url |
http://dx.doi.org/10.1089/mdr.2019.0038 http://hdl.handle.net/11449/185854 |
identifier_str_mv |
Microbial Drug Resistance. New Rochelle: Mary Ann Liebert, Inc, 11 p., 2019. 1076-6294 10.1089/mdr.2019.0038 WOS:000473305100001 3368404126695911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Drug Resistance |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert, Inc |
publisher.none.fl_str_mv |
Mary Ann Liebert, Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965719963631616 |