Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/186757 |
Resumo: | Statement of problem. The daily immersion of dentures in disinfectant solutions can cause the incorporation of toxic substances in the acrylic resins, and studies evaluating the cumulative effect of disinfectant solutions on cell culture are lacking. Purpose. The purpose of this in vitro study was to evaluate the cytotoxic potential of cell cultures of denture base and reline acrylic resins after immersion in disinfectant solutions. Material and methods. Disk-shaped specimens (14x1.2 mm) were obtained and divided into groups (n=9) according to the disinfectant solutions (distilled water [control], 2% chlorhexidine digluconate, 3.8% sodium perborate, 0.5% sodium hypochlorite, and apple vinegar) and to the storage period (0, 1, 3, and 6 months). Solutions were changed daily. After the different storage periods, specimens were immersed in culture medium for 24 hours, and extracts were obtained. Human keratinocytes were cultivated, and the cellular metabolism was evaluated by using Alamar Blue. Data were submitted to 3-way analysis of variance and Games-Howell post hoc tests (alpha=.05). Results. Both of the acrylic resins tested showed similar biocompatibility properties after immersion in different solutions (P=.400). Immersion in distilled water, 3.8% sodium perborate, and 0.5% sodium hypochlorite did not affect the cellular metabolism of the keratinocytes (P>.05), regardless of the immersion period and the type of acrylic resin (P>.05). Immersion in 2% chlorhexidine digluconate or apple vinegar resulted in high cytotoxicity over time, until the third month (P<.05), after which no changes were observed (P>.05). Conclusions. The type of acrylic resin (base or reline) had no significant effect on the viability of cells. Vinegar and chlorhexidine digluconate solutions increased in cytotoxic effect over time, and were strongly cytotoxic after 6 months of immersion. Sodium perborate and sodium hypochlorite were noncytotoxic in all periods of time tested. |
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Repositório Institucional da UNESP |
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spelling |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutionsStatement of problem. The daily immersion of dentures in disinfectant solutions can cause the incorporation of toxic substances in the acrylic resins, and studies evaluating the cumulative effect of disinfectant solutions on cell culture are lacking. Purpose. The purpose of this in vitro study was to evaluate the cytotoxic potential of cell cultures of denture base and reline acrylic resins after immersion in disinfectant solutions. Material and methods. Disk-shaped specimens (14x1.2 mm) were obtained and divided into groups (n=9) according to the disinfectant solutions (distilled water [control], 2% chlorhexidine digluconate, 3.8% sodium perborate, 0.5% sodium hypochlorite, and apple vinegar) and to the storage period (0, 1, 3, and 6 months). Solutions were changed daily. After the different storage periods, specimens were immersed in culture medium for 24 hours, and extracts were obtained. Human keratinocytes were cultivated, and the cellular metabolism was evaluated by using Alamar Blue. Data were submitted to 3-way analysis of variance and Games-Howell post hoc tests (alpha=.05). Results. Both of the acrylic resins tested showed similar biocompatibility properties after immersion in different solutions (P=.400). Immersion in distilled water, 3.8% sodium perborate, and 0.5% sodium hypochlorite did not affect the cellular metabolism of the keratinocytes (P>.05), regardless of the immersion period and the type of acrylic resin (P>.05). Immersion in 2% chlorhexidine digluconate or apple vinegar resulted in high cytotoxicity over time, until the third month (P<.05), after which no changes were observed (P>.05). Conclusions. The type of acrylic resin (base or reline) had no significant effect on the viability of cells. Vinegar and chlorhexidine digluconate solutions increased in cytotoxic effect over time, and were strongly cytotoxic after 6 months of immersion. Sodium perborate and sodium hypochlorite were noncytotoxic in all periods of time tested.Sao Paulo State Univ UNESP, Araraquara Dent Sch, Campus Araraqu,Rua Humaita 1680, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Araraquara Dent Sch, Campus Araraqu,Rua Humaita 1680, Araraquara, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Masetti, Paula [UNESP]Amaya Arbelaez, Maria Isabel [UNESP]Pavarina, Ana Claudia [UNESP]Sanita, Paula Volpato [UNESP]Jorge, Janaina Habib [UNESP]2019-10-06T02:14:22Z2019-10-06T02:14:22Z2018-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7Journal Of Prosthetic Dentistry. New York: Mosby-elsevier, v. 120, n. 1, 7 p., 2018.0022-3913http://hdl.handle.net/11449/186757WOS:000469125100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Prosthetic Dentistryinfo:eu-repo/semantics/openAccess2021-10-22T22:23:39Zoai:repositorio.unesp.br:11449/186757Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:08:37.154354Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
title |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
spellingShingle |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions Masetti, Paula [UNESP] |
title_short |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
title_full |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
title_fullStr |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
title_full_unstemmed |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
title_sort |
Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions |
author |
Masetti, Paula [UNESP] |
author_facet |
Masetti, Paula [UNESP] Amaya Arbelaez, Maria Isabel [UNESP] Pavarina, Ana Claudia [UNESP] Sanita, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
author_role |
author |
author2 |
Amaya Arbelaez, Maria Isabel [UNESP] Pavarina, Ana Claudia [UNESP] Sanita, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Masetti, Paula [UNESP] Amaya Arbelaez, Maria Isabel [UNESP] Pavarina, Ana Claudia [UNESP] Sanita, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
description |
Statement of problem. The daily immersion of dentures in disinfectant solutions can cause the incorporation of toxic substances in the acrylic resins, and studies evaluating the cumulative effect of disinfectant solutions on cell culture are lacking. Purpose. The purpose of this in vitro study was to evaluate the cytotoxic potential of cell cultures of denture base and reline acrylic resins after immersion in disinfectant solutions. Material and methods. Disk-shaped specimens (14x1.2 mm) were obtained and divided into groups (n=9) according to the disinfectant solutions (distilled water [control], 2% chlorhexidine digluconate, 3.8% sodium perborate, 0.5% sodium hypochlorite, and apple vinegar) and to the storage period (0, 1, 3, and 6 months). Solutions were changed daily. After the different storage periods, specimens were immersed in culture medium for 24 hours, and extracts were obtained. Human keratinocytes were cultivated, and the cellular metabolism was evaluated by using Alamar Blue. Data were submitted to 3-way analysis of variance and Games-Howell post hoc tests (alpha=.05). Results. Both of the acrylic resins tested showed similar biocompatibility properties after immersion in different solutions (P=.400). Immersion in distilled water, 3.8% sodium perborate, and 0.5% sodium hypochlorite did not affect the cellular metabolism of the keratinocytes (P>.05), regardless of the immersion period and the type of acrylic resin (P>.05). Immersion in 2% chlorhexidine digluconate or apple vinegar resulted in high cytotoxicity over time, until the third month (P<.05), after which no changes were observed (P>.05). Conclusions. The type of acrylic resin (base or reline) had no significant effect on the viability of cells. Vinegar and chlorhexidine digluconate solutions increased in cytotoxic effect over time, and were strongly cytotoxic after 6 months of immersion. Sodium perborate and sodium hypochlorite were noncytotoxic in all periods of time tested. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-01 2019-10-06T02:14:22Z 2019-10-06T02:14:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal Of Prosthetic Dentistry. New York: Mosby-elsevier, v. 120, n. 1, 7 p., 2018. 0022-3913 http://hdl.handle.net/11449/186757 WOS:000469125100001 |
identifier_str_mv |
Journal Of Prosthetic Dentistry. New York: Mosby-elsevier, v. 120, n. 1, 7 p., 2018. 0022-3913 WOS:000469125100001 |
url |
http://hdl.handle.net/11449/186757 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Prosthetic Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129396848984064 |