Análises bioquímicas e densitometria óssea de ratas ovariectomizadas submetidas à terapia de reposição hormonal

• Autor(a) principal: Prates, Lyvia Fernandes [UNESP]
• Data de Publicação: 2010
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149409; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-26/000851552.pdf
• topic: Remodelação óssea; Estradiol; Osteoporose; Menopausa; Ovariectomia; Bone remodeling

Estrogen is an important female sex hormone that regulates several physiological reactions. In postmenopausal women, the decrease of estrogen is associated with several phatologies, which include bone resorption. The effectiveness of hormone replacement therapy is established, whereas it reduces the symptoms of menopause, including preventing cardiovascular diseases. However, side effects like weight gain and risk of breast cancer and endometrial cancer are worrying. The purpose of this study was to determine systematically the dose-response effect estradiol on bone tissue of ovariectomized rats. Wistar rats (6 months) were selected after consideration of the estrous cycle and ovariectomized (OVX). 15 days after receiving pellets containing corn oil or 100, 200 or 300 μg of 17β-estradiol for 60 days. After this period, and under anesthesia (xylazina-4mg/Kg; ketamina-40mg/Kg), blood was collected from the jugular vein and then sacrificed by excess anesthesia for the removal of the femurs. The blood was centrifuged and plasma stored in a freezer for subsequent biochemical determinations of calcium, phosphorus and alkaline phosphatase. The femurs were subjected to analysis of bone density using digital Digora. The results shows that the optical density was lower in the femoral condyle in the group of animals that received 300μg/estradiol for 60 days. The plasma concentration of calcium was similar in both groups, phosphorus was significantly lower in the OVX group treated with 100 μg/Estradiol and alkaline phosphatase was lower in animals treated with 200 and 300 μg/Estradiol. These results suggests important differences between cortical and cancellous bone after treatment with estradiol