Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0007114520000896 http://hdl.handle.net/11449/200186 |
Resumo: | The aim of the present work was to compare the capacity to modulate the intestinal microbiota and the production of metabolites after 14 d administration of a commercial dietary supplement and a manufactured ice cream, both containing the same quantity of inulin and the same viable counts of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis BB-12, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model. Samples of the colonic contents were evaluated microbiologically by real-time quantitative PCR (qRT-PCR) and next-generation sequencing and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions (NH 4 ). Statistical analyses were carried out for all the variables using the two-way ANOVA followed by the Tukey multiple comparisons test (P < 0-05) for metabolite production, qRT-PCR and the bioinformatics analysis for microbiota diversity. Dietary supplement and ice cream were able to deliver the probiotic L. acidophilus and B. animalis to the simulated colon and modulate the microbiota, increasing beneficial micro-organisms such as Bifidobacterium spp., Bacteroides spp. and Faecalibacterium spp. for dietary supplement administration, and Lactobacillus spp. for ice cream supplementation. However, the ice cream matrix was probably more favourable for the maintenance of the metabolic activity of the probiotics in the SHIME® model, due to the larger amounts of acetate, propionate, butyrate and ammonium ions obtained after 14 d of supplementation. In conclusion, both ways of probiotic supplementation could be efficient, each with its own particularities. |
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Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probioticsAmmoniaBifidobacterium animalisFatty acidsLactobacillus acidophilusSHIME®The aim of the present work was to compare the capacity to modulate the intestinal microbiota and the production of metabolites after 14 d administration of a commercial dietary supplement and a manufactured ice cream, both containing the same quantity of inulin and the same viable counts of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis BB-12, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model. Samples of the colonic contents were evaluated microbiologically by real-time quantitative PCR (qRT-PCR) and next-generation sequencing and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions (NH 4 ). Statistical analyses were carried out for all the variables using the two-way ANOVA followed by the Tukey multiple comparisons test (P < 0-05) for metabolite production, qRT-PCR and the bioinformatics analysis for microbiota diversity. Dietary supplement and ice cream were able to deliver the probiotic L. acidophilus and B. animalis to the simulated colon and modulate the microbiota, increasing beneficial micro-organisms such as Bifidobacterium spp., Bacteroides spp. and Faecalibacterium spp. for dietary supplement administration, and Lactobacillus spp. for ice cream supplementation. However, the ice cream matrix was probably more favourable for the maintenance of the metabolic activity of the probiotics in the SHIME® model, due to the larger amounts of acetate, propionate, butyrate and ammonium ions obtained after 14 d of supplementation. In conclusion, both ways of probiotic supplementation could be efficient, each with its own particularities.School of Applied Sciences (FCA) State University of CampinasDepartment of Food and Nutrition School of Pharmaceutical Sciences S o Paulo State University (UNESP)Division of Organic and Pharmaceutical Chemistry Pluridisciplinary Center for Chemical Biological and Agricultural Research (CPQBA) State University of CampinasBrazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM)Genome Research Division Research Informatics Core Research Resource Center University of Illinois at ChicagoDepartment of Food and Nutrition School of Pharmaceutical Sciences S o Paulo State University (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Brazilian Center for Research in Energy and Materials (CNPEM)University of Illinois at ChicagoDa Cruz Rodrigues, Vivian CristinaRocha Faria Duque, Ana Luiza [UNESP]De Carvalho Fino, LucianaSimabuco, Fernando MoreiraSartoratto, AdilsonCabral, Luc liaNoronha, Melline FontesSivieri, Katia [UNESP]Antunes, Adriane Elisabete Costa2020-12-12T01:59:55Z2020-12-12T01:59:55Z2020-07-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article57-68http://dx.doi.org/10.1017/S0007114520000896British Journal of Nutrition, v. 124, n. 1, p. 57-68, 2020.1475-26620007-1145http://hdl.handle.net/11449/20018610.1017/S00071145200008962-s2.0-85081932656Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Nutritioninfo:eu-repo/semantics/openAccess2024-06-21T12:46:31Zoai:repositorio.unesp.br:11449/200186Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:46:13.305478Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
title |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
spellingShingle |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics Da Cruz Rodrigues, Vivian Cristina Ammonia Bifidobacterium animalis Fatty acids Lactobacillus acidophilus SHIME® |
title_short |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
title_full |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
title_fullStr |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
title_full_unstemmed |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
title_sort |
Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics |
author |
Da Cruz Rodrigues, Vivian Cristina |
author_facet |
Da Cruz Rodrigues, Vivian Cristina Rocha Faria Duque, Ana Luiza [UNESP] De Carvalho Fino, Luciana Simabuco, Fernando Moreira Sartoratto, Adilson Cabral, Luc lia Noronha, Melline Fontes Sivieri, Katia [UNESP] Antunes, Adriane Elisabete Costa |
author_role |
author |
author2 |
Rocha Faria Duque, Ana Luiza [UNESP] De Carvalho Fino, Luciana Simabuco, Fernando Moreira Sartoratto, Adilson Cabral, Luc lia Noronha, Melline Fontes Sivieri, Katia [UNESP] Antunes, Adriane Elisabete Costa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Brazilian Center for Research in Energy and Materials (CNPEM) University of Illinois at Chicago |
dc.contributor.author.fl_str_mv |
Da Cruz Rodrigues, Vivian Cristina Rocha Faria Duque, Ana Luiza [UNESP] De Carvalho Fino, Luciana Simabuco, Fernando Moreira Sartoratto, Adilson Cabral, Luc lia Noronha, Melline Fontes Sivieri, Katia [UNESP] Antunes, Adriane Elisabete Costa |
dc.subject.por.fl_str_mv |
Ammonia Bifidobacterium animalis Fatty acids Lactobacillus acidophilus SHIME® |
topic |
Ammonia Bifidobacterium animalis Fatty acids Lactobacillus acidophilus SHIME® |
description |
The aim of the present work was to compare the capacity to modulate the intestinal microbiota and the production of metabolites after 14 d administration of a commercial dietary supplement and a manufactured ice cream, both containing the same quantity of inulin and the same viable counts of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis BB-12, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model. Samples of the colonic contents were evaluated microbiologically by real-time quantitative PCR (qRT-PCR) and next-generation sequencing and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions (NH 4 ). Statistical analyses were carried out for all the variables using the two-way ANOVA followed by the Tukey multiple comparisons test (P < 0-05) for metabolite production, qRT-PCR and the bioinformatics analysis for microbiota diversity. Dietary supplement and ice cream were able to deliver the probiotic L. acidophilus and B. animalis to the simulated colon and modulate the microbiota, increasing beneficial micro-organisms such as Bifidobacterium spp., Bacteroides spp. and Faecalibacterium spp. for dietary supplement administration, and Lactobacillus spp. for ice cream supplementation. However, the ice cream matrix was probably more favourable for the maintenance of the metabolic activity of the probiotics in the SHIME® model, due to the larger amounts of acetate, propionate, butyrate and ammonium ions obtained after 14 d of supplementation. In conclusion, both ways of probiotic supplementation could be efficient, each with its own particularities. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:59:55Z 2020-12-12T01:59:55Z 2020-07-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0007114520000896 British Journal of Nutrition, v. 124, n. 1, p. 57-68, 2020. 1475-2662 0007-1145 http://hdl.handle.net/11449/200186 10.1017/S0007114520000896 2-s2.0-85081932656 |
url |
http://dx.doi.org/10.1017/S0007114520000896 http://hdl.handle.net/11449/200186 |
identifier_str_mv |
British Journal of Nutrition, v. 124, n. 1, p. 57-68, 2020. 1475-2662 0007-1145 10.1017/S0007114520000896 2-s2.0-85081932656 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
British Journal of Nutrition |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
57-68 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128274296995840 |