Embryonic development of teleost Brycon orbignyanus
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0967199418000229 http://hdl.handle.net/11449/185004 |
Resumo: | Brycon orbignyanus is an important large teleost that is currently on the list of endangered species, therefore studies on its reproductive biology and embryology arc fundamental to help species conservation and recovery. The objective of this research was to characterize the events that occur during extrusion, fertilization and embryonic development of the species. The samples were collected at predetermined times, fixed and processed for light microscopy and scanning electron microscopy. The greenish oocytes were spherical, had translucent chorion and a mean diameter of 1.3 + 0.11 mm. The eggs had well defined animal and vegetative poles approximately 18 min post-fertilization. Stages from 2 to 128 blastomeres occurred between 20 min and 3 h post-fertilization (hPF) when the morula was Characterized. The blastula stage was observed between 2 and 3 hPF, and the gastrula between 3 and 7 hPF, when the embryonic shield emerged and the cellular migration with the consequent formation of epiblast and hypoblast. At 8 hPF the formation of the neural tube, above the notochord and the encephalic region, was observed, delimiting the forebrain, mesencephalon and rhombencephalon regions. From 11 hPF onward, the optic vesicle was formed close to the forebrain and the embryo tail was well developed. The optic vesicle was observed from 12 hPF onwnrd, and the tail showed an intense movement that culminated with the rupture of the chorion and consequent hatching of the larva at 13 hPF and 27 degrees C. |
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Embryonic development of teleost Brycon orbignyanusEmbryologyFishHistologyReproductionUltrastructureBrycon orbignyanus is an important large teleost that is currently on the list of endangered species, therefore studies on its reproductive biology and embryology arc fundamental to help species conservation and recovery. The objective of this research was to characterize the events that occur during extrusion, fertilization and embryonic development of the species. The samples were collected at predetermined times, fixed and processed for light microscopy and scanning electron microscopy. The greenish oocytes were spherical, had translucent chorion and a mean diameter of 1.3 + 0.11 mm. The eggs had well defined animal and vegetative poles approximately 18 min post-fertilization. Stages from 2 to 128 blastomeres occurred between 20 min and 3 h post-fertilization (hPF) when the morula was Characterized. The blastula stage was observed between 2 and 3 hPF, and the gastrula between 3 and 7 hPF, when the embryonic shield emerged and the cellular migration with the consequent formation of epiblast and hypoblast. At 8 hPF the formation of the neural tube, above the notochord and the encephalic region, was observed, delimiting the forebrain, mesencephalon and rhombencephalon regions. From 11 hPF onward, the optic vesicle was formed close to the forebrain and the embryo tail was well developed. The optic vesicle was observed from 12 hPF onwnrd, and the tail showed an intense movement that culminated with the rupture of the chorion and consequent hatching of the larva at 13 hPF and 27 degrees C.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Embrapa, Caixa Postal 90, BR-77001970 Palmas, TO, BrazilAquaculture Ctr Sao Paulo State Univ, Jaboticabai, SP, BrazilSao Paulo State Univ, Dept Biol Sci, Bauru, SP, BrazilSao Paulo State Univ, Dept Anim Morphol & Physiol, Jaboticabai, SP, BrazilAquaculture Ctr Sao Paulo State Univ, Jaboticabai, SP, BrazilSao Paulo State Univ, Dept Biol Sci, Bauru, SP, BrazilSao Paulo State Univ, Dept Anim Morphol & Physiol, Jaboticabai, SP, BrazilCambridge Univ PressEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Ganeco-Kirschnik, Luciana Nakaghi [UNESP]Franceschini-Vicentini, Irene Bastos [UNESP]Faria Paes, Maria do Carmo [UNESP]Okada Nakaghi, Laura Satiko [UNESP]2019-10-04T12:31:51Z2019-10-04T12:31:51Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article294-300http://dx.doi.org/10.1017/S0967199418000229Zygote. New York: Cambridge Univ Press, v. 26, n. 4, p. 294-300, 2018.0967-1994http://hdl.handle.net/11449/18500410.1017/S0967199418000229WOS:000448811000006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygoteinfo:eu-repo/semantics/openAccess2024-04-23T15:23:16Zoai:repositorio.unesp.br:11449/185004Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-23T15:23:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Embryonic development of teleost Brycon orbignyanus |
title |
Embryonic development of teleost Brycon orbignyanus |
spellingShingle |
Embryonic development of teleost Brycon orbignyanus Ganeco-Kirschnik, Luciana Nakaghi [UNESP] Embryology Fish Histology Reproduction Ultrastructure |
title_short |
Embryonic development of teleost Brycon orbignyanus |
title_full |
Embryonic development of teleost Brycon orbignyanus |
title_fullStr |
Embryonic development of teleost Brycon orbignyanus |
title_full_unstemmed |
Embryonic development of teleost Brycon orbignyanus |
title_sort |
Embryonic development of teleost Brycon orbignyanus |
author |
Ganeco-Kirschnik, Luciana Nakaghi [UNESP] |
author_facet |
Ganeco-Kirschnik, Luciana Nakaghi [UNESP] Franceschini-Vicentini, Irene Bastos [UNESP] Faria Paes, Maria do Carmo [UNESP] Okada Nakaghi, Laura Satiko [UNESP] |
author_role |
author |
author2 |
Franceschini-Vicentini, Irene Bastos [UNESP] Faria Paes, Maria do Carmo [UNESP] Okada Nakaghi, Laura Satiko [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ganeco-Kirschnik, Luciana Nakaghi [UNESP] Franceschini-Vicentini, Irene Bastos [UNESP] Faria Paes, Maria do Carmo [UNESP] Okada Nakaghi, Laura Satiko [UNESP] |
dc.subject.por.fl_str_mv |
Embryology Fish Histology Reproduction Ultrastructure |
topic |
Embryology Fish Histology Reproduction Ultrastructure |
description |
Brycon orbignyanus is an important large teleost that is currently on the list of endangered species, therefore studies on its reproductive biology and embryology arc fundamental to help species conservation and recovery. The objective of this research was to characterize the events that occur during extrusion, fertilization and embryonic development of the species. The samples were collected at predetermined times, fixed and processed for light microscopy and scanning electron microscopy. The greenish oocytes were spherical, had translucent chorion and a mean diameter of 1.3 + 0.11 mm. The eggs had well defined animal and vegetative poles approximately 18 min post-fertilization. Stages from 2 to 128 blastomeres occurred between 20 min and 3 h post-fertilization (hPF) when the morula was Characterized. The blastula stage was observed between 2 and 3 hPF, and the gastrula between 3 and 7 hPF, when the embryonic shield emerged and the cellular migration with the consequent formation of epiblast and hypoblast. At 8 hPF the formation of the neural tube, above the notochord and the encephalic region, was observed, delimiting the forebrain, mesencephalon and rhombencephalon regions. From 11 hPF onward, the optic vesicle was formed close to the forebrain and the embryo tail was well developed. The optic vesicle was observed from 12 hPF onwnrd, and the tail showed an intense movement that culminated with the rupture of the chorion and consequent hatching of the larva at 13 hPF and 27 degrees C. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-01 2019-10-04T12:31:51Z 2019-10-04T12:31:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199418000229 Zygote. New York: Cambridge Univ Press, v. 26, n. 4, p. 294-300, 2018. 0967-1994 http://hdl.handle.net/11449/185004 10.1017/S0967199418000229 WOS:000448811000006 |
url |
http://dx.doi.org/10.1017/S0967199418000229 http://hdl.handle.net/11449/185004 |
identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 26, n. 4, p. 294-300, 2018. 0967-1994 10.1017/S0967199418000229 WOS:000448811000006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
294-300 |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046109591896064 |