Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols

Detalhes bibliográficos
Autor(a) principal: Roza de Abreu, Mariana [UNESP]
Data de Publicação: 2021
Outros Autores: Silva, Laíza Maria de Jesus [UNESP], Figueiredo-Ariki, Daniel Guimarães [UNESP], Sato, Rafael Tomoda [UNESP], Kuradomi, Rafael Yutaka [UNESP], Batlouni, Sergio Ricardo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/are.14905
http://hdl.handle.net/11449/206578
Resumo: We evaluated lambari (Astyanax altiparanae) reproductive performance by administration of gradually increasing doses of analogue of salmon gonadotropin-releasing hormone (sGnRHa). We performed two consecutive experiments applying six sGnRHa single doses in three replicates (ranging from 1 to 160 µg/kg) (Exp. 1) and three sGnRHa-fractioned doses in four replicates (10% + 90% – 12 hr interval) (10, 100 and 1,000 µg/kg) (Exp. 2) in 6 months old breeders. In both experiments, 6 mg of Carp pituitary extract (CPE)/kg and saline solution (without hormonal therapy) were used as positive and negative controls, respectively. Reproductive performance was recorded for both experiments; and quantification of 17α-20β-dihydroxy-4-pregnen-3-one (17,20β-P) and prostaglandin F2α plasma levels and ovarian histological evaluations were introduced in Exp. 2. Using a single dose, the spawning rate was low and similar between all groups (from 0% to 20%). Using fractioned doses, the spawning rate of CPE (60%) was higher than other groups (from 10% to 25%), but similar to 1,000 µg of sGnRHa/kg (40%). 17,20β-P increased at the final sampling regardless of treatment (including controls) or successful spawning, suggesting that the simple grouping of fish caused this increase. 17,20β-P and PGF2α plasma levels did not explain ovulation failures in this species. CPE-fractioned doses improved lambari ovulation and provided final maturation in all evaluated females; however, hormonal induction was not conditional for obtaining ovulation since spawning was registered in negative control. Concluding, CPE-fractioned doses protocol can be applied to increase reproductive performance of this species.
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spelling Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocolsbrood-stock managementcontrolled reproductionegg maturation and ovulationovarian developmentprostaglandinsex steroids hormonesWe evaluated lambari (Astyanax altiparanae) reproductive performance by administration of gradually increasing doses of analogue of salmon gonadotropin-releasing hormone (sGnRHa). We performed two consecutive experiments applying six sGnRHa single doses in three replicates (ranging from 1 to 160 µg/kg) (Exp. 1) and three sGnRHa-fractioned doses in four replicates (10% + 90% – 12 hr interval) (10, 100 and 1,000 µg/kg) (Exp. 2) in 6 months old breeders. In both experiments, 6 mg of Carp pituitary extract (CPE)/kg and saline solution (without hormonal therapy) were used as positive and negative controls, respectively. Reproductive performance was recorded for both experiments; and quantification of 17α-20β-dihydroxy-4-pregnen-3-one (17,20β-P) and prostaglandin F2α plasma levels and ovarian histological evaluations were introduced in Exp. 2. Using a single dose, the spawning rate was low and similar between all groups (from 0% to 20%). Using fractioned doses, the spawning rate of CPE (60%) was higher than other groups (from 10% to 25%), but similar to 1,000 µg of sGnRHa/kg (40%). 17,20β-P increased at the final sampling regardless of treatment (including controls) or successful spawning, suggesting that the simple grouping of fish caused this increase. 17,20β-P and PGF2α plasma levels did not explain ovulation failures in this species. CPE-fractioned doses improved lambari ovulation and provided final maturation in all evaluated females; however, hormonal induction was not conditional for obtaining ovulation since spawning was registered in negative control. Concluding, CPE-fractioned doses protocol can be applied to increase reproductive performance of this species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESPEmbrapa Amazônia Ocidental, Rodovia AM-010, Km 29, s/n, Zona Rural – CEP: 69010-970, Caixa Postal 319Instituto de Ciências Exatas e Tecnologia (ICET) Universidade Federal do Amazonas (UFAM)Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESPFAPESP: 2016/25988-7FAPESP: 2019/05741-5Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal do Amazonas (UFAM)Roza de Abreu, Mariana [UNESP]Silva, Laíza Maria de Jesus [UNESP]Figueiredo-Ariki, Daniel Guimarães [UNESP]Sato, Rafael Tomoda [UNESP]Kuradomi, Rafael Yutaka [UNESP]Batlouni, Sergio Ricardo [UNESP]2021-06-25T10:34:38Z2021-06-25T10:34:38Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article471-483http://dx.doi.org/10.1111/are.14905Aquaculture Research, v. 52, n. 2, p. 471-483, 2021.1365-21091355-557Xhttp://hdl.handle.net/11449/20657810.1111/are.149052-s2.0-85091684375Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture Researchinfo:eu-repo/semantics/openAccess2024-04-09T15:29:46Zoai:repositorio.unesp.br:11449/206578Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:08:13.030474Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
title Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
spellingShingle Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
Roza de Abreu, Mariana [UNESP]
brood-stock management
controlled reproduction
egg maturation and ovulation
ovarian development
prostaglandin
sex steroids hormones
title_short Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
title_full Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
title_fullStr Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
title_full_unstemmed Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
title_sort Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols
author Roza de Abreu, Mariana [UNESP]
author_facet Roza de Abreu, Mariana [UNESP]
Silva, Laíza Maria de Jesus [UNESP]
Figueiredo-Ariki, Daniel Guimarães [UNESP]
Sato, Rafael Tomoda [UNESP]
Kuradomi, Rafael Yutaka [UNESP]
Batlouni, Sergio Ricardo [UNESP]
author_role author
author2 Silva, Laíza Maria de Jesus [UNESP]
Figueiredo-Ariki, Daniel Guimarães [UNESP]
Sato, Rafael Tomoda [UNESP]
Kuradomi, Rafael Yutaka [UNESP]
Batlouni, Sergio Ricardo [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal do Amazonas (UFAM)
dc.contributor.author.fl_str_mv Roza de Abreu, Mariana [UNESP]
Silva, Laíza Maria de Jesus [UNESP]
Figueiredo-Ariki, Daniel Guimarães [UNESP]
Sato, Rafael Tomoda [UNESP]
Kuradomi, Rafael Yutaka [UNESP]
Batlouni, Sergio Ricardo [UNESP]
dc.subject.por.fl_str_mv brood-stock management
controlled reproduction
egg maturation and ovulation
ovarian development
prostaglandin
sex steroids hormones
topic brood-stock management
controlled reproduction
egg maturation and ovulation
ovarian development
prostaglandin
sex steroids hormones
description We evaluated lambari (Astyanax altiparanae) reproductive performance by administration of gradually increasing doses of analogue of salmon gonadotropin-releasing hormone (sGnRHa). We performed two consecutive experiments applying six sGnRHa single doses in three replicates (ranging from 1 to 160 µg/kg) (Exp. 1) and three sGnRHa-fractioned doses in four replicates (10% + 90% – 12 hr interval) (10, 100 and 1,000 µg/kg) (Exp. 2) in 6 months old breeders. In both experiments, 6 mg of Carp pituitary extract (CPE)/kg and saline solution (without hormonal therapy) were used as positive and negative controls, respectively. Reproductive performance was recorded for both experiments; and quantification of 17α-20β-dihydroxy-4-pregnen-3-one (17,20β-P) and prostaglandin F2α plasma levels and ovarian histological evaluations were introduced in Exp. 2. Using a single dose, the spawning rate was low and similar between all groups (from 0% to 20%). Using fractioned doses, the spawning rate of CPE (60%) was higher than other groups (from 10% to 25%), but similar to 1,000 µg of sGnRHa/kg (40%). 17,20β-P increased at the final sampling regardless of treatment (including controls) or successful spawning, suggesting that the simple grouping of fish caused this increase. 17,20β-P and PGF2α plasma levels did not explain ovulation failures in this species. CPE-fractioned doses improved lambari ovulation and provided final maturation in all evaluated females; however, hormonal induction was not conditional for obtaining ovulation since spawning was registered in negative control. Concluding, CPE-fractioned doses protocol can be applied to increase reproductive performance of this species.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:34:38Z
2021-06-25T10:34:38Z
2021-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/are.14905
Aquaculture Research, v. 52, n. 2, p. 471-483, 2021.
1365-2109
1355-557X
http://hdl.handle.net/11449/206578
10.1111/are.14905
2-s2.0-85091684375
url http://dx.doi.org/10.1111/are.14905
http://hdl.handle.net/11449/206578
identifier_str_mv Aquaculture Research, v. 52, n. 2, p. 471-483, 2021.
1365-2109
1355-557X
10.1111/are.14905
2-s2.0-85091684375
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquaculture Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 471-483
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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