The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1677-04202002000200003 http://hdl.handle.net/11449/67080 |
Resumo: | Coffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25°C) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80% acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results indicated a generalized reduction in the quantum yield of PSII when the seedlings were maintained in the dark. The reduction occurred in the seedlings submitted to chilling treatment as well as in the control ones. This demonstrates that not only chilling acts to cause an alteration in PSII. It is possible that the tissue storage reserves had been totally exhausted, with the respiratory rate exceeding the photosynthetic rate; the later was nil, since the seedlings were kept in the dark. The efficiency in the capture, transfer and utilization of light energy in PS11 photochemical reactions requires a sequence of photochemical, biochemical and biophysical events which depend on the structural integrity of the photosynthetic apparatus. However, this efficiency was found to be related to the protective action of chloroplastid pigments, rather than to the concentration of these pigments. |
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The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigmentsChlorophyll a fluorescenceCoffeeLipid peroxidationPhotosynthetic pigmentsCoffea arabicaCoffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25°C) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80% acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results indicated a generalized reduction in the quantum yield of PSII when the seedlings were maintained in the dark. The reduction occurred in the seedlings submitted to chilling treatment as well as in the control ones. This demonstrates that not only chilling acts to cause an alteration in PSII. It is possible that the tissue storage reserves had been totally exhausted, with the respiratory rate exceeding the photosynthetic rate; the later was nil, since the seedlings were kept in the dark. The efficiency in the capture, transfer and utilization of light energy in PS11 photochemical reactions requires a sequence of photochemical, biochemical and biophysical events which depend on the structural integrity of the photosynthetic apparatus. However, this efficiency was found to be related to the protective action of chloroplastid pigments, rather than to the concentration of these pigments.Lab. de Melhoramento Genetico Veg. Ctro. Cie. Tecnologias Agropecuarias Univ. Estadual do Notre Fluminense, CEP 28013-600, Campos dos GoytacazesDepto. de Biol. Apl. a Agropecuaria Faculdade Cie. Agrarias Veterinarias Univ. Estadual de São Paulo, CEP 14870-000, Jaboticabal, SPDepartamento de Fisiologia Vegetal Instituto de Biologia Universidade Estadual de Campinas, 13083-970 Campinas, SPUniversidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Oliveira, Jurandi Gonçalves deAlves, Pedro Luis C. A.Magalhães, Antonio Celso2014-05-27T11:20:33Z2014-05-27T11:20:33Z2002-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-104application/pdfhttp://dx.doi.org/10.1590/S1677-04202002000200003Brasilian Journal of Plant Physiology, v. 14, n. 2, p. 95-104, 2002.1677-0420http://hdl.handle.net/11449/6708010.1590/S1677-04202002000200003S1677-042020020002000032-s2.0-00370020162-s2.0-0037002016.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Plant Physiology0,123info:eu-repo/semantics/openAccess2023-10-06T06:01:29Zoai:repositorio.unesp.br:11449/67080Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462023-10-06T06:01:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
title |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
spellingShingle |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments Oliveira, Jurandi Gonçalves de Chlorophyll a fluorescence Coffee Lipid peroxidation Photosynthetic pigments Coffea arabica |
title_short |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
title_full |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
title_fullStr |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
title_full_unstemmed |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
title_sort |
The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments |
author |
Oliveira, Jurandi Gonçalves de |
author_facet |
Oliveira, Jurandi Gonçalves de Alves, Pedro Luis C. A. Magalhães, Antonio Celso |
author_role |
author |
author2 |
Alves, Pedro Luis C. A. Magalhães, Antonio Celso |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Oliveira, Jurandi Gonçalves de Alves, Pedro Luis C. A. Magalhães, Antonio Celso |
dc.subject.por.fl_str_mv |
Chlorophyll a fluorescence Coffee Lipid peroxidation Photosynthetic pigments Coffea arabica |
topic |
Chlorophyll a fluorescence Coffee Lipid peroxidation Photosynthetic pigments Coffea arabica |
description |
Coffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25°C) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80% acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results indicated a generalized reduction in the quantum yield of PSII when the seedlings were maintained in the dark. The reduction occurred in the seedlings submitted to chilling treatment as well as in the control ones. This demonstrates that not only chilling acts to cause an alteration in PSII. It is possible that the tissue storage reserves had been totally exhausted, with the respiratory rate exceeding the photosynthetic rate; the later was nil, since the seedlings were kept in the dark. The efficiency in the capture, transfer and utilization of light energy in PS11 photochemical reactions requires a sequence of photochemical, biochemical and biophysical events which depend on the structural integrity of the photosynthetic apparatus. However, this efficiency was found to be related to the protective action of chloroplastid pigments, rather than to the concentration of these pigments. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12-01 2014-05-27T11:20:33Z 2014-05-27T11:20:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1677-04202002000200003 Brasilian Journal of Plant Physiology, v. 14, n. 2, p. 95-104, 2002. 1677-0420 http://hdl.handle.net/11449/67080 10.1590/S1677-04202002000200003 S1677-04202002000200003 2-s2.0-0037002016 2-s2.0-0037002016.pdf |
url |
http://dx.doi.org/10.1590/S1677-04202002000200003 http://hdl.handle.net/11449/67080 |
identifier_str_mv |
Brasilian Journal of Plant Physiology, v. 14, n. 2, p. 95-104, 2002. 1677-0420 10.1590/S1677-04202002000200003 S1677-04202002000200003 2-s2.0-0037002016 2-s2.0-0037002016.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Plant Physiology 0,123 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
95-104 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1826303515317764096 |