The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments

Detalhes bibliográficos
Autor(a) principal: Oliveira, Jurandi Gonçalves de
Data de Publicação: 2002
Outros Autores: Alves, Pedro Luis C. A., Magalhães, Antonio Celso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1677-04202002000200003
http://hdl.handle.net/11449/67080
Resumo: Coffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25°C) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80% acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results indicated a generalized reduction in the quantum yield of PSII when the seedlings were maintained in the dark. The reduction occurred in the seedlings submitted to chilling treatment as well as in the control ones. This demonstrates that not only chilling acts to cause an alteration in PSII. It is possible that the tissue storage reserves had been totally exhausted, with the respiratory rate exceeding the photosynthetic rate; the later was nil, since the seedlings were kept in the dark. The efficiency in the capture, transfer and utilization of light energy in PS11 photochemical reactions requires a sequence of photochemical, biochemical and biophysical events which depend on the structural integrity of the photosynthetic apparatus. However, this efficiency was found to be related to the protective action of chloroplastid pigments, rather than to the concentration of these pigments.
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spelling The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigmentsChlorophyll a fluorescenceCoffeeLipid peroxidationPhotosynthetic pigmentsCoffea arabicaCoffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25°C) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80% acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results indicated a generalized reduction in the quantum yield of PSII when the seedlings were maintained in the dark. The reduction occurred in the seedlings submitted to chilling treatment as well as in the control ones. This demonstrates that not only chilling acts to cause an alteration in PSII. It is possible that the tissue storage reserves had been totally exhausted, with the respiratory rate exceeding the photosynthetic rate; the later was nil, since the seedlings were kept in the dark. The efficiency in the capture, transfer and utilization of light energy in PS11 photochemical reactions requires a sequence of photochemical, biochemical and biophysical events which depend on the structural integrity of the photosynthetic apparatus. However, this efficiency was found to be related to the protective action of chloroplastid pigments, rather than to the concentration of these pigments.Lab. de Melhoramento Genetico Veg. Ctro. Cie. Tecnologias Agropecuarias Univ. Estadual do Notre Fluminense, CEP 28013-600, Campos dos GoytacazesDepto. de Biol. Apl. a Agropecuaria Faculdade Cie. Agrarias Veterinarias Univ. Estadual de São Paulo, CEP 14870-000, Jaboticabal, SPDepartamento de Fisiologia Vegetal Instituto de Biologia Universidade Estadual de Campinas, 13083-970 Campinas, SPUniversidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Oliveira, Jurandi Gonçalves deAlves, Pedro Luis C. A.Magalhães, Antonio Celso2014-05-27T11:20:33Z2014-05-27T11:20:33Z2002-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-104application/pdfhttp://dx.doi.org/10.1590/S1677-04202002000200003Brasilian Journal of Plant Physiology, v. 14, n. 2, p. 95-104, 2002.1677-0420http://hdl.handle.net/11449/6708010.1590/S1677-04202002000200003S1677-042020020002000032-s2.0-00370020162-s2.0-0037002016.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Plant Physiology0,123info:eu-repo/semantics/openAccess2023-10-06T06:01:29Zoai:repositorio.unesp.br:11449/67080Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462023-10-06T06:01:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
title The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
spellingShingle The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
Oliveira, Jurandi Gonçalves de
Chlorophyll a fluorescence
Coffee
Lipid peroxidation
Photosynthetic pigments
Coffea arabica
title_short The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
title_full The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
title_fullStr The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
title_full_unstemmed The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
title_sort The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings. The protective action of chloroplastid pigments
author Oliveira, Jurandi Gonçalves de
author_facet Oliveira, Jurandi Gonçalves de
Alves, Pedro Luis C. A.
Magalhães, Antonio Celso
author_role author
author2 Alves, Pedro Luis C. A.
Magalhães, Antonio Celso
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Oliveira, Jurandi Gonçalves de
Alves, Pedro Luis C. A.
Magalhães, Antonio Celso
dc.subject.por.fl_str_mv Chlorophyll a fluorescence
Coffee
Lipid peroxidation
Photosynthetic pigments
Coffea arabica
topic Chlorophyll a fluorescence
Coffee
Lipid peroxidation
Photosynthetic pigments
Coffea arabica
description Coffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25°C) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80% acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results indicated a generalized reduction in the quantum yield of PSII when the seedlings were maintained in the dark. The reduction occurred in the seedlings submitted to chilling treatment as well as in the control ones. This demonstrates that not only chilling acts to cause an alteration in PSII. It is possible that the tissue storage reserves had been totally exhausted, with the respiratory rate exceeding the photosynthetic rate; the later was nil, since the seedlings were kept in the dark. The efficiency in the capture, transfer and utilization of light energy in PS11 photochemical reactions requires a sequence of photochemical, biochemical and biophysical events which depend on the structural integrity of the photosynthetic apparatus. However, this efficiency was found to be related to the protective action of chloroplastid pigments, rather than to the concentration of these pigments.
publishDate 2002
dc.date.none.fl_str_mv 2002-12-01
2014-05-27T11:20:33Z
2014-05-27T11:20:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1677-04202002000200003
Brasilian Journal of Plant Physiology, v. 14, n. 2, p. 95-104, 2002.
1677-0420
http://hdl.handle.net/11449/67080
10.1590/S1677-04202002000200003
S1677-04202002000200003
2-s2.0-0037002016
2-s2.0-0037002016.pdf
url http://dx.doi.org/10.1590/S1677-04202002000200003
http://hdl.handle.net/11449/67080
identifier_str_mv Brasilian Journal of Plant Physiology, v. 14, n. 2, p. 95-104, 2002.
1677-0420
10.1590/S1677-04202002000200003
S1677-04202002000200003
2-s2.0-0037002016
2-s2.0-0037002016.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Plant Physiology
0,123
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 95-104
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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