Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient

Detalhes bibliográficos
Autor(a) principal: Camargo, Rosalinda Yasato
Data de Publicação: 2018
Outros Autores: Kanamura, Cristina Takami, Friguglietti, Celso Ubirajara, Nogueira, Célia Regina [UNESP], Iorcansky, Sonia, Tincani, Alfio José, Bezerra, Ana Karina, Brust, Ester, Koyama, Fernanda Christtanini, Camargo, Anamaria Aranha, Rego, Fernanda Orpinelli R., Galante, Pedro Alexandre Favoretto, Medeiros-Neto, Geraldo, Rubio, Ileana Gabriela Sanchez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2018/4682876
http://hdl.handle.net/11449/175931
Resumo: Ectopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients.
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spelling Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult PatientEctopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients.Thyroid Unit Cellular and Molecular Endocrine Laboratory LIM-25 Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Doutor Arnaldo 455, Cerqueira CésarAdolfo Lutz Institute São Paulo Public Health Service, Av. Dr. Arnaldo 355, Cerqueira CésarHead and Neck Surgery of Santa Catarina Hospital, Av. Paulista 200, Bela VistaDepartment of Internal Medicine Botucatu School of Medicine UNESP, Av. Prof. Montenegro, s/n Distrito de Rubião JuniorServicio de Endocrinología Hospital de Pediatría Dr. Juan Garrahan, Combate de los Pozos 1881Departamento de Cirurgia Na Disciplina de Cirurgia de Cabeça e Pescoço Faculdade de Ciências Médicas UNICAMP, R. Tessália Vieira de Camargo 126Medicine School Universidade de Fortaleza (Unifor), Av. Washington Soares 1321, Edson QueirozPostgraduate Program in Biotechnology Universidade Federal de São Paulo (UNIFESP), Pedro de Toledo 669Thyroid Molecular Sciences Laboratory Universidade Federal de São Paulo Departamento de Ciências Biológicas Postgraduation Programs in Biotechnology and Structural and Functional Biology UNIFESP, Pedro de Toledo 669Molecular Oncology Center Hospital Sírio-Libanés, Rua Prof. Daher Cutait 69Department of Internal Medicine Botucatu School of Medicine UNESP, Av. Prof. Montenegro, s/n Distrito de Rubião JuniorUniversidade de São Paulo (USP)São Paulo Public Health ServiceHead and Neck Surgery of Santa Catarina HospitalUniversidade Estadual Paulista (Unesp)Hospital de Pediatría Dr. Juan GarrahanUniversidade Estadual de Campinas (UNICAMP)Universidade de Fortaleza (Unifor)Universidade Federal de São Paulo (UNIFESP)Hospital Sírio-LibanésCamargo, Rosalinda YasatoKanamura, Cristina TakamiFriguglietti, Celso UbirajaraNogueira, Célia Regina [UNESP]Iorcansky, SoniaTincani, Alfio JoséBezerra, Ana KarinaBrust, EsterKoyama, Fernanda ChristtaniniCamargo, Anamaria AranhaRego, Fernanda Orpinelli R.Galante, Pedro Alexandre FavorettoMedeiros-Neto, GeraldoRubio, Ileana Gabriela Sanchez2018-12-11T17:18:12Z2018-12-11T17:18:12Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2018/4682876International Journal of Endocrinology, v. 2018.1687-83451687-8337http://hdl.handle.net/11449/17593110.1155/2018/46828762-s2.0-850424695142-s2.0-85042469514.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Endocrinology1,0121,012info:eu-repo/semantics/openAccess2024-08-14T17:37:05Zoai:repositorio.unesp.br:11449/175931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:37:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
title Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
spellingShingle Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
Camargo, Rosalinda Yasato
title_short Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
title_full Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
title_fullStr Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
title_full_unstemmed Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
title_sort Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
author Camargo, Rosalinda Yasato
author_facet Camargo, Rosalinda Yasato
Kanamura, Cristina Takami
Friguglietti, Celso Ubirajara
Nogueira, Célia Regina [UNESP]
Iorcansky, Sonia
Tincani, Alfio José
Bezerra, Ana Karina
Brust, Ester
Koyama, Fernanda Christtanini
Camargo, Anamaria Aranha
Rego, Fernanda Orpinelli R.
Galante, Pedro Alexandre Favoretto
Medeiros-Neto, Geraldo
Rubio, Ileana Gabriela Sanchez
author_role author
author2 Kanamura, Cristina Takami
Friguglietti, Celso Ubirajara
Nogueira, Célia Regina [UNESP]
Iorcansky, Sonia
Tincani, Alfio José
Bezerra, Ana Karina
Brust, Ester
Koyama, Fernanda Christtanini
Camargo, Anamaria Aranha
Rego, Fernanda Orpinelli R.
Galante, Pedro Alexandre Favoretto
Medeiros-Neto, Geraldo
Rubio, Ileana Gabriela Sanchez
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
São Paulo Public Health Service
Head and Neck Surgery of Santa Catarina Hospital
Universidade Estadual Paulista (Unesp)
Hospital de Pediatría Dr. Juan Garrahan
Universidade Estadual de Campinas (UNICAMP)
Universidade de Fortaleza (Unifor)
Universidade Federal de São Paulo (UNIFESP)
Hospital Sírio-Libanés
dc.contributor.author.fl_str_mv Camargo, Rosalinda Yasato
Kanamura, Cristina Takami
Friguglietti, Celso Ubirajara
Nogueira, Célia Regina [UNESP]
Iorcansky, Sonia
Tincani, Alfio José
Bezerra, Ana Karina
Brust, Ester
Koyama, Fernanda Christtanini
Camargo, Anamaria Aranha
Rego, Fernanda Orpinelli R.
Galante, Pedro Alexandre Favoretto
Medeiros-Neto, Geraldo
Rubio, Ileana Gabriela Sanchez
description Ectopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:18:12Z
2018-12-11T17:18:12Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2018/4682876
International Journal of Endocrinology, v. 2018.
1687-8345
1687-8337
http://hdl.handle.net/11449/175931
10.1155/2018/4682876
2-s2.0-85042469514
2-s2.0-85042469514.pdf
url http://dx.doi.org/10.1155/2018/4682876
http://hdl.handle.net/11449/175931
identifier_str_mv International Journal of Endocrinology, v. 2018.
1687-8345
1687-8337
10.1155/2018/4682876
2-s2.0-85042469514
2-s2.0-85042469514.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Endocrinology
1,012
1,012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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