Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2018/4682876 http://hdl.handle.net/11449/175931 |
Resumo: | Ectopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients. |
id |
UNSP_95f7af70ec44b978c167cd355b8d95f2 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/175931 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult PatientEctopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients.Thyroid Unit Cellular and Molecular Endocrine Laboratory LIM-25 Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Doutor Arnaldo 455, Cerqueira CésarAdolfo Lutz Institute São Paulo Public Health Service, Av. Dr. Arnaldo 355, Cerqueira CésarHead and Neck Surgery of Santa Catarina Hospital, Av. Paulista 200, Bela VistaDepartment of Internal Medicine Botucatu School of Medicine UNESP, Av. Prof. Montenegro, s/n Distrito de Rubião JuniorServicio de Endocrinología Hospital de Pediatría Dr. Juan Garrahan, Combate de los Pozos 1881Departamento de Cirurgia Na Disciplina de Cirurgia de Cabeça e Pescoço Faculdade de Ciências Médicas UNICAMP, R. Tessália Vieira de Camargo 126Medicine School Universidade de Fortaleza (Unifor), Av. Washington Soares 1321, Edson QueirozPostgraduate Program in Biotechnology Universidade Federal de São Paulo (UNIFESP), Pedro de Toledo 669Thyroid Molecular Sciences Laboratory Universidade Federal de São Paulo Departamento de Ciências Biológicas Postgraduation Programs in Biotechnology and Structural and Functional Biology UNIFESP, Pedro de Toledo 669Molecular Oncology Center Hospital Sírio-Libanés, Rua Prof. Daher Cutait 69Department of Internal Medicine Botucatu School of Medicine UNESP, Av. Prof. Montenegro, s/n Distrito de Rubião JuniorUniversidade de São Paulo (USP)São Paulo Public Health ServiceHead and Neck Surgery of Santa Catarina HospitalUniversidade Estadual Paulista (Unesp)Hospital de Pediatría Dr. Juan GarrahanUniversidade Estadual de Campinas (UNICAMP)Universidade de Fortaleza (Unifor)Universidade Federal de São Paulo (UNIFESP)Hospital Sírio-LibanésCamargo, Rosalinda YasatoKanamura, Cristina TakamiFriguglietti, Celso UbirajaraNogueira, Célia Regina [UNESP]Iorcansky, SoniaTincani, Alfio JoséBezerra, Ana KarinaBrust, EsterKoyama, Fernanda ChristtaniniCamargo, Anamaria AranhaRego, Fernanda Orpinelli R.Galante, Pedro Alexandre FavorettoMedeiros-Neto, GeraldoRubio, Ileana Gabriela Sanchez2018-12-11T17:18:12Z2018-12-11T17:18:12Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2018/4682876International Journal of Endocrinology, v. 2018.1687-83451687-8337http://hdl.handle.net/11449/17593110.1155/2018/46828762-s2.0-850424695142-s2.0-85042469514.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Endocrinology1,0121,012info:eu-repo/semantics/openAccess2024-08-14T17:37:05Zoai:repositorio.unesp.br:11449/175931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:37:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
title |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
spellingShingle |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient Camargo, Rosalinda Yasato |
title_short |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
title_full |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
title_fullStr |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
title_full_unstemmed |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
title_sort |
Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
author |
Camargo, Rosalinda Yasato |
author_facet |
Camargo, Rosalinda Yasato Kanamura, Cristina Takami Friguglietti, Celso Ubirajara Nogueira, Célia Regina [UNESP] Iorcansky, Sonia Tincani, Alfio José Bezerra, Ana Karina Brust, Ester Koyama, Fernanda Christtanini Camargo, Anamaria Aranha Rego, Fernanda Orpinelli R. Galante, Pedro Alexandre Favoretto Medeiros-Neto, Geraldo Rubio, Ileana Gabriela Sanchez |
author_role |
author |
author2 |
Kanamura, Cristina Takami Friguglietti, Celso Ubirajara Nogueira, Célia Regina [UNESP] Iorcansky, Sonia Tincani, Alfio José Bezerra, Ana Karina Brust, Ester Koyama, Fernanda Christtanini Camargo, Anamaria Aranha Rego, Fernanda Orpinelli R. Galante, Pedro Alexandre Favoretto Medeiros-Neto, Geraldo Rubio, Ileana Gabriela Sanchez |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) São Paulo Public Health Service Head and Neck Surgery of Santa Catarina Hospital Universidade Estadual Paulista (Unesp) Hospital de Pediatría Dr. Juan Garrahan Universidade Estadual de Campinas (UNICAMP) Universidade de Fortaleza (Unifor) Universidade Federal de São Paulo (UNIFESP) Hospital Sírio-Libanés |
dc.contributor.author.fl_str_mv |
Camargo, Rosalinda Yasato Kanamura, Cristina Takami Friguglietti, Celso Ubirajara Nogueira, Célia Regina [UNESP] Iorcansky, Sonia Tincani, Alfio José Bezerra, Ana Karina Brust, Ester Koyama, Fernanda Christtanini Camargo, Anamaria Aranha Rego, Fernanda Orpinelli R. Galante, Pedro Alexandre Favoretto Medeiros-Neto, Geraldo Rubio, Ileana Gabriela Sanchez |
description |
Ectopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:12Z 2018-12-11T17:18:12Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2018/4682876 International Journal of Endocrinology, v. 2018. 1687-8345 1687-8337 http://hdl.handle.net/11449/175931 10.1155/2018/4682876 2-s2.0-85042469514 2-s2.0-85042469514.pdf |
url |
http://dx.doi.org/10.1155/2018/4682876 http://hdl.handle.net/11449/175931 |
identifier_str_mv |
International Journal of Endocrinology, v. 2018. 1687-8345 1687-8337 10.1155/2018/4682876 2-s2.0-85042469514 2-s2.0-85042469514.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Endocrinology 1,012 1,012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128215330324480 |