Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2023.139073 http://hdl.handle.net/11449/249100 |
Resumo: | Pseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m−2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m−2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L−1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems. |
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Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cellAcetic acidAnodic oxidationBiodieselButyric acidExternal resistanceGlycerolPseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m−2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m−2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L−1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de São Paulo- Faculdade de Filosofia Ciências e Letras de Ribeirão Preto – FFCLRP – SP. Departamento de Química, Av. Bandeirantes, 3900, SPUniversidade de São Paulo - Faculdade de Ciências Farmacêuticas de Ribeirão Preto – FCFRP – SP. Departamento de Análises Clínicas Toxicológicas e Bromatológicas, Av. Bandeirantes, 3900, SPUniversidade de São Paulo - Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Departamento de Biologia, Av. Bandeirantes 3900, SPUnesp National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry, P.O. Box 355, SPUnesp National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry, P.O. Box 355, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Zani, Ana Clara BonizolAlmeida, Érica Janaina Rodrigues deFurlan, João Pedro RuedaPedrino, MatheusGuazzaroni, María-EugeniaStehling, Eliana GuedesAndrade, Adalgisa Rodrigues de [UNESP]Reginatto, Valeria2023-07-29T14:02:26Z2023-07-29T14:02:26Z2023-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.chemosphere.2023.139073Chemosphere, v. 335.1879-12980045-6535http://hdl.handle.net/11449/24910010.1016/j.chemosphere.2023.1390732-s2.0-85161026590Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2024-06-24T14:51:41Zoai:repositorio.unesp.br:11449/249100Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:19:24.280877Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
title |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
spellingShingle |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell Zani, Ana Clara Bonizol Acetic acid Anodic oxidation Biodiesel Butyric acid External resistance Glycerol |
title_short |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
title_full |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
title_fullStr |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
title_full_unstemmed |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
title_sort |
Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell |
author |
Zani, Ana Clara Bonizol |
author_facet |
Zani, Ana Clara Bonizol Almeida, Érica Janaina Rodrigues de Furlan, João Pedro Rueda Pedrino, Matheus Guazzaroni, María-Eugenia Stehling, Eliana Guedes Andrade, Adalgisa Rodrigues de [UNESP] Reginatto, Valeria |
author_role |
author |
author2 |
Almeida, Érica Janaina Rodrigues de Furlan, João Pedro Rueda Pedrino, Matheus Guazzaroni, María-Eugenia Stehling, Eliana Guedes Andrade, Adalgisa Rodrigues de [UNESP] Reginatto, Valeria |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Zani, Ana Clara Bonizol Almeida, Érica Janaina Rodrigues de Furlan, João Pedro Rueda Pedrino, Matheus Guazzaroni, María-Eugenia Stehling, Eliana Guedes Andrade, Adalgisa Rodrigues de [UNESP] Reginatto, Valeria |
dc.subject.por.fl_str_mv |
Acetic acid Anodic oxidation Biodiesel Butyric acid External resistance Glycerol |
topic |
Acetic acid Anodic oxidation Biodiesel Butyric acid External resistance Glycerol |
description |
Pseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m−2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m−2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L−1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T14:02:26Z 2023-07-29T14:02:26Z 2023-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2023.139073 Chemosphere, v. 335. 1879-1298 0045-6535 http://hdl.handle.net/11449/249100 10.1016/j.chemosphere.2023.139073 2-s2.0-85161026590 |
url |
http://dx.doi.org/10.1016/j.chemosphere.2023.139073 http://hdl.handle.net/11449/249100 |
identifier_str_mv |
Chemosphere, v. 335. 1879-1298 0045-6535 10.1016/j.chemosphere.2023.139073 2-s2.0-85161026590 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128919444914176 |