Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell

Detalhes bibliográficos
Autor(a) principal: Zani, Ana Clara Bonizol
Data de Publicação: 2023
Outros Autores: Almeida, Érica Janaina Rodrigues de, Furlan, João Pedro Rueda, Pedrino, Matheus, Guazzaroni, María-Eugenia, Stehling, Eliana Guedes, Andrade, Adalgisa Rodrigues de [UNESP], Reginatto, Valeria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.chemosphere.2023.139073
http://hdl.handle.net/11449/249100
Resumo: Pseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m−2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m−2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L−1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems.
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spelling Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cellAcetic acidAnodic oxidationBiodieselButyric acidExternal resistanceGlycerolPseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m−2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m−2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L−1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de São Paulo- Faculdade de Filosofia Ciências e Letras de Ribeirão Preto – FFCLRP – SP. Departamento de Química, Av. Bandeirantes, 3900, SPUniversidade de São Paulo - Faculdade de Ciências Farmacêuticas de Ribeirão Preto – FCFRP – SP. Departamento de Análises Clínicas Toxicológicas e Bromatológicas, Av. Bandeirantes, 3900, SPUniversidade de São Paulo - Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Departamento de Biologia, Av. Bandeirantes 3900, SPUnesp National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry, P.O. Box 355, SPUnesp National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry, P.O. Box 355, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Zani, Ana Clara BonizolAlmeida, Érica Janaina Rodrigues deFurlan, João Pedro RuedaPedrino, MatheusGuazzaroni, María-EugeniaStehling, Eliana GuedesAndrade, Adalgisa Rodrigues de [UNESP]Reginatto, Valeria2023-07-29T14:02:26Z2023-07-29T14:02:26Z2023-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.chemosphere.2023.139073Chemosphere, v. 335.1879-12980045-6535http://hdl.handle.net/11449/24910010.1016/j.chemosphere.2023.1390732-s2.0-85161026590Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2024-06-24T14:51:41Zoai:repositorio.unesp.br:11449/249100Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:19:24.280877Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
title Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
spellingShingle Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
Zani, Ana Clara Bonizol
Acetic acid
Anodic oxidation
Biodiesel
Butyric acid
External resistance
Glycerol
title_short Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
title_full Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
title_fullStr Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
title_full_unstemmed Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
title_sort Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell
author Zani, Ana Clara Bonizol
author_facet Zani, Ana Clara Bonizol
Almeida, Érica Janaina Rodrigues de
Furlan, João Pedro Rueda
Pedrino, Matheus
Guazzaroni, María-Eugenia
Stehling, Eliana Guedes
Andrade, Adalgisa Rodrigues de [UNESP]
Reginatto, Valeria
author_role author
author2 Almeida, Érica Janaina Rodrigues de
Furlan, João Pedro Rueda
Pedrino, Matheus
Guazzaroni, María-Eugenia
Stehling, Eliana Guedes
Andrade, Adalgisa Rodrigues de [UNESP]
Reginatto, Valeria
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Zani, Ana Clara Bonizol
Almeida, Érica Janaina Rodrigues de
Furlan, João Pedro Rueda
Pedrino, Matheus
Guazzaroni, María-Eugenia
Stehling, Eliana Guedes
Andrade, Adalgisa Rodrigues de [UNESP]
Reginatto, Valeria
dc.subject.por.fl_str_mv Acetic acid
Anodic oxidation
Biodiesel
Butyric acid
External resistance
Glycerol
topic Acetic acid
Anodic oxidation
Biodiesel
Butyric acid
External resistance
Glycerol
description Pseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m−2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m−2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L−1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T14:02:26Z
2023-07-29T14:02:26Z
2023-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.chemosphere.2023.139073
Chemosphere, v. 335.
1879-1298
0045-6535
http://hdl.handle.net/11449/249100
10.1016/j.chemosphere.2023.139073
2-s2.0-85161026590
url http://dx.doi.org/10.1016/j.chemosphere.2023.139073
http://hdl.handle.net/11449/249100
identifier_str_mv Chemosphere, v. 335.
1879-1298
0045-6535
10.1016/j.chemosphere.2023.139073
2-s2.0-85161026590
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemosphere
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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