Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/cid/cix558 http://hdl.handle.net/11449/163340 |
Resumo: | Background. The pathogenesis of severe dengue disease involves immune components as biomarkers. The mechanism by which some dengue virus (DENV)-infected individuals progress to severe disease is poorly understood. Most studies on the pathogenesis of severe dengue disease focus on the process of antibody-dependent enhancement (ADE) as a primary risk factor. With the circulation of Zika virus (ZIKV) in DENV-endemic areas, many people infected by ZIKV were likely exposed to DENV. The influence of such exposure on Zika disease outcomes remains unknown. Methods. We investigated whether patients previously exposed to DENV exhibited higher viremia when exposed to a subsequent, heterologous dengue or Zika infection than those patients not previously exposed to dengue. We measured viral loads and cytokine profile during patients' acute infections. Results. Neither dengue nor Zika viremia was higher in patients with prior DENV infection, although the power to detect such a difference was only adequate in the ZIKV analysis. Of the 10 cytokines measured, only 1 significant difference was detected: Levels of interleukin 1 beta (IL-1 beta) were lower in dengue-infected patients who had experienced a previous dengue infection than patients infected with dengue for the first time. However, power to detect differences between groups was low. In Zika-infected patients, levels of IL-1 beta showed a significant, positive correlation with viral load. Conclusions. No signs of ADE were observed in vivo in patients with acute ZIKV infection who had prior exposure to DENV. |
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Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected PatientsZIKVDENVADEcytokinesBackground. The pathogenesis of severe dengue disease involves immune components as biomarkers. The mechanism by which some dengue virus (DENV)-infected individuals progress to severe disease is poorly understood. Most studies on the pathogenesis of severe dengue disease focus on the process of antibody-dependent enhancement (ADE) as a primary risk factor. With the circulation of Zika virus (ZIKV) in DENV-endemic areas, many people infected by ZIKV were likely exposed to DENV. The influence of such exposure on Zika disease outcomes remains unknown. Methods. We investigated whether patients previously exposed to DENV exhibited higher viremia when exposed to a subsequent, heterologous dengue or Zika infection than those patients not previously exposed to dengue. We measured viral loads and cytokine profile during patients' acute infections. Results. Neither dengue nor Zika viremia was higher in patients with prior DENV infection, although the power to detect such a difference was only adequate in the ZIKV analysis. Of the 10 cytokines measured, only 1 significant difference was detected: Levels of interleukin 1 beta (IL-1 beta) were lower in dengue-infected patients who had experienced a previous dengue infection than patients infected with dengue for the first time. However, power to detect differences between groups was low. In Zika-infected patients, levels of IL-1 beta showed a significant, positive correlation with viral load. Conclusions. No signs of ADE were observed in vivo in patients with acute ZIKV infection who had prior exposure to DENV.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Butantan InstituteBrazilian National Institutes for Science and Technology for Dengue Studies (INCT em Dengue)National Institutes of HealthSao Paulo State Univ, Sao Jose do Rio Preto Sch Med, Sao Jose Do Rio Preto, BrazilSao Paulo State Univ, Butantan Inst, Sao Jose Do Rio Preto, BrazilSao Paulo State Univ, Dept Biol, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, BrazilNew Mexico State Univ, Las Cruces, NM 88003 USAUniv Texas Med Branch, Galveston, TX 77555 USAUniv Sao Paulo, Sch Med, Sao Paulo, BrazilSao Paulo State Univ, Sao Jose do Rio Preto Sch Med, Sao Jose Do Rio Preto, BrazilSao Paulo State Univ, Butantan Inst, Sao Jose Do Rio Preto, BrazilSao Paulo State Univ, Dept Biol, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, BrazilFAPESP: 2013/21719-3FAPESP: 2015/12295-0National Institutes of Health: 1U01AI115577-01Oxford Univ Press IncUniversidade Estadual Paulista (Unesp)New Mexico State UnivUniv Texas Med BranchUniversidade de São Paulo (USP)Bernardes Terzian, Ana Carolina [UNESP]Schanoski, Alessandra Soares [UNESP]Oliveira Mota, Minh' Tasso de [UNESP]Silva, Rafael Alves da [UNESP]Estofolete, Cassia Fernanda [UNESP]Colombo, Tatiana Elias [UNESP]Rahal, Paula [UNESP]Hanley, Kathryn A.Vasilakis, NikosKalil, JorgeNogueiral, Mauricio Lacerda [UNESP]2018-11-26T17:41:03Z2018-11-26T17:41:03Z2017-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1260-1265application/pdfhttp://dx.doi.org/10.1093/cid/cix558Clinical Infectious Diseases. Cary: Oxford Univ Press Inc, v. 65, n. 8, p. 1260-1265, 2017.1058-4838http://hdl.handle.net/11449/16334010.1093/cid/cix558WOS:000412022500002WOS000412022500002.pdf79910823626712120000-0001-5693-6148Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Infectious Diseases5,051info:eu-repo/semantics/openAccess2024-01-19T06:33:08Zoai:repositorio.unesp.br:11449/163340Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-19T06:33:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
title |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
spellingShingle |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients Bernardes Terzian, Ana Carolina [UNESP] ZIKV DENV ADE cytokines |
title_short |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
title_full |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
title_fullStr |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
title_full_unstemmed |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
title_sort |
Viral Load and Cytokine Response Profile Does Not Support Antibody-Dependent Enhancement in Dengue-Primed Zika Virus-Infected Patients |
author |
Bernardes Terzian, Ana Carolina [UNESP] |
author_facet |
Bernardes Terzian, Ana Carolina [UNESP] Schanoski, Alessandra Soares [UNESP] Oliveira Mota, Minh' Tasso de [UNESP] Silva, Rafael Alves da [UNESP] Estofolete, Cassia Fernanda [UNESP] Colombo, Tatiana Elias [UNESP] Rahal, Paula [UNESP] Hanley, Kathryn A. Vasilakis, Nikos Kalil, Jorge Nogueiral, Mauricio Lacerda [UNESP] |
author_role |
author |
author2 |
Schanoski, Alessandra Soares [UNESP] Oliveira Mota, Minh' Tasso de [UNESP] Silva, Rafael Alves da [UNESP] Estofolete, Cassia Fernanda [UNESP] Colombo, Tatiana Elias [UNESP] Rahal, Paula [UNESP] Hanley, Kathryn A. Vasilakis, Nikos Kalil, Jorge Nogueiral, Mauricio Lacerda [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) New Mexico State Univ Univ Texas Med Branch Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Bernardes Terzian, Ana Carolina [UNESP] Schanoski, Alessandra Soares [UNESP] Oliveira Mota, Minh' Tasso de [UNESP] Silva, Rafael Alves da [UNESP] Estofolete, Cassia Fernanda [UNESP] Colombo, Tatiana Elias [UNESP] Rahal, Paula [UNESP] Hanley, Kathryn A. Vasilakis, Nikos Kalil, Jorge Nogueiral, Mauricio Lacerda [UNESP] |
dc.subject.por.fl_str_mv |
ZIKV DENV ADE cytokines |
topic |
ZIKV DENV ADE cytokines |
description |
Background. The pathogenesis of severe dengue disease involves immune components as biomarkers. The mechanism by which some dengue virus (DENV)-infected individuals progress to severe disease is poorly understood. Most studies on the pathogenesis of severe dengue disease focus on the process of antibody-dependent enhancement (ADE) as a primary risk factor. With the circulation of Zika virus (ZIKV) in DENV-endemic areas, many people infected by ZIKV were likely exposed to DENV. The influence of such exposure on Zika disease outcomes remains unknown. Methods. We investigated whether patients previously exposed to DENV exhibited higher viremia when exposed to a subsequent, heterologous dengue or Zika infection than those patients not previously exposed to dengue. We measured viral loads and cytokine profile during patients' acute infections. Results. Neither dengue nor Zika viremia was higher in patients with prior DENV infection, although the power to detect such a difference was only adequate in the ZIKV analysis. Of the 10 cytokines measured, only 1 significant difference was detected: Levels of interleukin 1 beta (IL-1 beta) were lower in dengue-infected patients who had experienced a previous dengue infection than patients infected with dengue for the first time. However, power to detect differences between groups was low. In Zika-infected patients, levels of IL-1 beta showed a significant, positive correlation with viral load. Conclusions. No signs of ADE were observed in vivo in patients with acute ZIKV infection who had prior exposure to DENV. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-15 2018-11-26T17:41:03Z 2018-11-26T17:41:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/cid/cix558 Clinical Infectious Diseases. Cary: Oxford Univ Press Inc, v. 65, n. 8, p. 1260-1265, 2017. 1058-4838 http://hdl.handle.net/11449/163340 10.1093/cid/cix558 WOS:000412022500002 WOS000412022500002.pdf 7991082362671212 0000-0001-5693-6148 |
url |
http://dx.doi.org/10.1093/cid/cix558 http://hdl.handle.net/11449/163340 |
identifier_str_mv |
Clinical Infectious Diseases. Cary: Oxford Univ Press Inc, v. 65, n. 8, p. 1260-1265, 2017. 1058-4838 10.1093/cid/cix558 WOS:000412022500002 WOS000412022500002.pdf 7991082362671212 0000-0001-5693-6148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Infectious Diseases 5,051 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1260-1265 application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Univ Press Inc |
publisher.none.fl_str_mv |
Oxford Univ Press Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965667169927168 |