Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules25092134 http://hdl.handle.net/11449/200375 |
Resumo: | Ageratina havanensis (Kunth) R. M. King & H. Robinson is a species of flowering shrub in the family Asteraceae, native to the Caribbean and Texas. The aim of this work was to compare the quantitative chemical composition of extracts obtained from Ageratina havanensis in its flowering and vegetative stages with the antioxidant potential and to determine the effects on P-glycoprotein (P-gp) function. The quantitative chemical composition of the extracts was determined quantifying their major flavonoids by UPLC-ESI-MS/MS and by PCA analysis. The effects of the extracts on P-gp activity was evaluated by Rhodamine 123 assay; antioxidant properties were determined by DPPH, FRAP and inhibition of lipid peroxidation methods. The obtained results show that major flavonoids were present in higher concentrations in vegetative stage than flowering stage. In particular, the extracts obtained in the flowering season showed a significantly higher ability to sequester free radicals compared to those of the vegetative season, meanwhile, the extracts obtained during the vegetative stage showed a significant inhibitory effect against brain lipid peroxidation and a strong reductive capacity. This study also showed the inhibitory effects of all ethanolic extracts on P-gp function in 4T1 cell line; these effects were unrelated to the phenological stage. This work shows, therefore, the first evidence on: The inhibition of P-gp function, the antioxidant effects and the content of major flavonoids of Ageratina havanensis. According to the obtained results, the species Ageratina havanensis (Kunth) R. M. King & H. Robinson could be a source of new potential inhibitors of drug efflux mediated by P-gp. A special focus on all these aspects must be taking into account for future studies. |
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Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein functionAgeratina havanensisAntioxidant potentialFlavonoidsP-glycoproteinUPLC-ESI-MS/MSAgeratina havanensis (Kunth) R. M. King & H. Robinson is a species of flowering shrub in the family Asteraceae, native to the Caribbean and Texas. The aim of this work was to compare the quantitative chemical composition of extracts obtained from Ageratina havanensis in its flowering and vegetative stages with the antioxidant potential and to determine the effects on P-glycoprotein (P-gp) function. The quantitative chemical composition of the extracts was determined quantifying their major flavonoids by UPLC-ESI-MS/MS and by PCA analysis. The effects of the extracts on P-gp activity was evaluated by Rhodamine 123 assay; antioxidant properties were determined by DPPH, FRAP and inhibition of lipid peroxidation methods. The obtained results show that major flavonoids were present in higher concentrations in vegetative stage than flowering stage. In particular, the extracts obtained in the flowering season showed a significantly higher ability to sequester free radicals compared to those of the vegetative season, meanwhile, the extracts obtained during the vegetative stage showed a significant inhibitory effect against brain lipid peroxidation and a strong reductive capacity. This study also showed the inhibitory effects of all ethanolic extracts on P-gp function in 4T1 cell line; these effects were unrelated to the phenological stage. This work shows, therefore, the first evidence on: The inhibition of P-gp function, the antioxidant effects and the content of major flavonoids of Ageratina havanensis. According to the obtained results, the species Ageratina havanensis (Kunth) R. M. King & H. Robinson could be a source of new potential inhibitors of drug efflux mediated by P-gp. A special focus on all these aspects must be taking into account for future studies.Center for Natural Product Research Faculty of Chemistry University of HavanaDepartment of Chemistry Federal University of MaranhãoDepartment of Pharmacology Institute of Marine Sciences (ICIMAR), Loma 14 Alturas del Vedado Plaza de la RevoluciónInstitute of Ecology and SystematicsLaboratory of Antitumor Natural Substances Department of Pharmacology Institute of Biological Sciences Federal University of Minas Gerais (UFMG)Experimental Campus of Sao Vicente UNESP-Sao Paulo State UniversityDepartment of Pharmacy University of Salerno, Via Giovanni Paolo IIExperimental Campus of Sao Vicente UNESP-Sao Paulo State UniversityUniversity of HavanaFederal University of MaranhãoInstitute of Marine Sciences (ICIMAR)Institute of Ecology and SystematicsUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)University of SalernoGarcía, Trina H.Da Rocha, Claudia QuintinoDelgado-Roche, LivanRodeiro, IdaniaÁvila, YaiserHernández, IvonesCuellar, CindelLopes, Miriam Teresa PazVilegas, Wagner [UNESP]Auriemma, GiuliaSpengler, IraidaRastrelli, Luca2020-12-12T02:04:59Z2020-12-12T02:04:59Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules25092134Molecules, v. 25, n. 9, 2020.1420-3049http://hdl.handle.net/11449/20037510.3390/molecules250921342-s2.0-85084276622Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2021-10-23T12:39:35Zoai:repositorio.unesp.br:11449/200375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:05:21.591365Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
title |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
spellingShingle |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function García, Trina H. Ageratina havanensis Antioxidant potential Flavonoids P-glycoprotein UPLC-ESI-MS/MS |
title_short |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
title_full |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
title_fullStr |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
title_full_unstemmed |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
title_sort |
Influence of the phenological state of in the antioxidant potential and chemical composition of ageratina havanensis. effects on the p-glycoprotein function |
author |
García, Trina H. |
author_facet |
García, Trina H. Da Rocha, Claudia Quintino Delgado-Roche, Livan Rodeiro, Idania Ávila, Yaiser Hernández, Ivones Cuellar, Cindel Lopes, Miriam Teresa Paz Vilegas, Wagner [UNESP] Auriemma, Giulia Spengler, Iraida Rastrelli, Luca |
author_role |
author |
author2 |
Da Rocha, Claudia Quintino Delgado-Roche, Livan Rodeiro, Idania Ávila, Yaiser Hernández, Ivones Cuellar, Cindel Lopes, Miriam Teresa Paz Vilegas, Wagner [UNESP] Auriemma, Giulia Spengler, Iraida Rastrelli, Luca |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Havana Federal University of Maranhão Institute of Marine Sciences (ICIMAR) Institute of Ecology and Systematics Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (Unesp) University of Salerno |
dc.contributor.author.fl_str_mv |
García, Trina H. Da Rocha, Claudia Quintino Delgado-Roche, Livan Rodeiro, Idania Ávila, Yaiser Hernández, Ivones Cuellar, Cindel Lopes, Miriam Teresa Paz Vilegas, Wagner [UNESP] Auriemma, Giulia Spengler, Iraida Rastrelli, Luca |
dc.subject.por.fl_str_mv |
Ageratina havanensis Antioxidant potential Flavonoids P-glycoprotein UPLC-ESI-MS/MS |
topic |
Ageratina havanensis Antioxidant potential Flavonoids P-glycoprotein UPLC-ESI-MS/MS |
description |
Ageratina havanensis (Kunth) R. M. King & H. Robinson is a species of flowering shrub in the family Asteraceae, native to the Caribbean and Texas. The aim of this work was to compare the quantitative chemical composition of extracts obtained from Ageratina havanensis in its flowering and vegetative stages with the antioxidant potential and to determine the effects on P-glycoprotein (P-gp) function. The quantitative chemical composition of the extracts was determined quantifying their major flavonoids by UPLC-ESI-MS/MS and by PCA analysis. The effects of the extracts on P-gp activity was evaluated by Rhodamine 123 assay; antioxidant properties were determined by DPPH, FRAP and inhibition of lipid peroxidation methods. The obtained results show that major flavonoids were present in higher concentrations in vegetative stage than flowering stage. In particular, the extracts obtained in the flowering season showed a significantly higher ability to sequester free radicals compared to those of the vegetative season, meanwhile, the extracts obtained during the vegetative stage showed a significant inhibitory effect against brain lipid peroxidation and a strong reductive capacity. This study also showed the inhibitory effects of all ethanolic extracts on P-gp function in 4T1 cell line; these effects were unrelated to the phenological stage. This work shows, therefore, the first evidence on: The inhibition of P-gp function, the antioxidant effects and the content of major flavonoids of Ageratina havanensis. According to the obtained results, the species Ageratina havanensis (Kunth) R. M. King & H. Robinson could be a source of new potential inhibitors of drug efflux mediated by P-gp. A special focus on all these aspects must be taking into account for future studies. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:04:59Z 2020-12-12T02:04:59Z 2020-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules25092134 Molecules, v. 25, n. 9, 2020. 1420-3049 http://hdl.handle.net/11449/200375 10.3390/molecules25092134 2-s2.0-85084276622 |
url |
http://dx.doi.org/10.3390/molecules25092134 http://hdl.handle.net/11449/200375 |
identifier_str_mv |
Molecules, v. 25, n. 9, 2020. 1420-3049 10.3390/molecules25092134 2-s2.0-85084276622 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129283078488064 |