Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/8626987 http://hdl.handle.net/11449/173539 |
Resumo: | Due to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogen Porphyromonas gingivalis by using Galleria mellonella as in vivo model. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension of P. gingivalis (106 cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox, P < 0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action against P. gingivalis in vivo model using G. mellonella. |
id |
UNSP_97215d8947164f8ad9c5559c7f452af3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/173539 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella modelDue to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogen Porphyromonas gingivalis by using Galleria mellonella as in vivo model. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension of P. gingivalis (106 cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox, P < 0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action against P. gingivalis in vivo model using G. mellonella.Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777Universidade Estadual Paulista (Unesp)Aparecida Procópio Gomes, Livia [UNESP]Alves Figueiredo, Lívia Mara [UNESP]Luiza Do Rosário Palma, Ana [UNESP]Corrêa Geraldo, Barbara Maria [UNESP]Isler Castro, Kelly Cristine [UNESP]Ruano De Oliveira Fugisaki, Luciana [UNESP]Olavo Cardoso Jorge, Antônio [UNESP]Dias De Oliveira, Luciane [UNESP]Campos Junqueira, Juliana [UNESP]2018-12-11T17:06:09Z2018-12-11T17:06:09Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/8626987Scientific World Journal, v. 2016.1537-744X2356-6140http://hdl.handle.net/11449/17353910.1155/2016/86269872-s2.0-849889749982-s2.0-84988974998.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific World Journalinfo:eu-repo/semantics/openAccess2024-01-19T06:27:04Zoai:repositorio.unesp.br:11449/173539Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:29.472598Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
title |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
spellingShingle |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model Aparecida Procópio Gomes, Livia [UNESP] |
title_short |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
title_full |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
title_fullStr |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
title_full_unstemmed |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
title_sort |
Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model |
author |
Aparecida Procópio Gomes, Livia [UNESP] |
author_facet |
Aparecida Procópio Gomes, Livia [UNESP] Alves Figueiredo, Lívia Mara [UNESP] Luiza Do Rosário Palma, Ana [UNESP] Corrêa Geraldo, Barbara Maria [UNESP] Isler Castro, Kelly Cristine [UNESP] Ruano De Oliveira Fugisaki, Luciana [UNESP] Olavo Cardoso Jorge, Antônio [UNESP] Dias De Oliveira, Luciane [UNESP] Campos Junqueira, Juliana [UNESP] |
author_role |
author |
author2 |
Alves Figueiredo, Lívia Mara [UNESP] Luiza Do Rosário Palma, Ana [UNESP] Corrêa Geraldo, Barbara Maria [UNESP] Isler Castro, Kelly Cristine [UNESP] Ruano De Oliveira Fugisaki, Luciana [UNESP] Olavo Cardoso Jorge, Antônio [UNESP] Dias De Oliveira, Luciane [UNESP] Campos Junqueira, Juliana [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Aparecida Procópio Gomes, Livia [UNESP] Alves Figueiredo, Lívia Mara [UNESP] Luiza Do Rosário Palma, Ana [UNESP] Corrêa Geraldo, Barbara Maria [UNESP] Isler Castro, Kelly Cristine [UNESP] Ruano De Oliveira Fugisaki, Luciana [UNESP] Olavo Cardoso Jorge, Antônio [UNESP] Dias De Oliveira, Luciane [UNESP] Campos Junqueira, Juliana [UNESP] |
description |
Due to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogen Porphyromonas gingivalis by using Galleria mellonella as in vivo model. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension of P. gingivalis (106 cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox, P < 0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action against P. gingivalis in vivo model using G. mellonella. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:06:09Z 2018-12-11T17:06:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/8626987 Scientific World Journal, v. 2016. 1537-744X 2356-6140 http://hdl.handle.net/11449/173539 10.1155/2016/8626987 2-s2.0-84988974998 2-s2.0-84988974998.pdf |
url |
http://dx.doi.org/10.1155/2016/8626987 http://hdl.handle.net/11449/173539 |
identifier_str_mv |
Scientific World Journal, v. 2016. 1537-744X 2356-6140 10.1155/2016/8626987 2-s2.0-84988974998 2-s2.0-84988974998.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific World Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129514769743872 |