CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE

Detalhes bibliográficos
Autor(a) principal: CONRADO, Pollyanna Cristina Vincenzi
Data de Publicação: 2019
Outros Autores: SAKITA, Karina Mayumi, ARITA, Glaucia Sayuri, GALINARI, Camila Barros, BONFIM-MENDONÇA, Patrícia de Souza, KIOSHIMA, Erika Seki
Tipo de documento: Artigo
Título da fonte: Repositório Digital Unicesumar
Texto Completo: http://rdu.unicesumar.edu.br//handle/123456789/3677
Resumo: Photodynamic inactivation (PDI) is a non-invasive technology, recognized since 1900, that uses a combination of a light source and a photosensitizer (PS), causing photochemical reactions. Hypericin (Hyp) is a napthodianthrone naturally isolated compound, with promising photodynamic properties by high penetration and absorption. A novel formulation was developed the encapsulated Hyp in P-123 copolymeric micelles (P123-Hyp) formed at low copolymeric concentration, with the intention to improve the photodynamic characteristics of Hyp. Therefore, its necessary evaluate if PDI mediated P-123-Hyp cause cytotoxicity in cell line human. For this, assay was performed to evaluate the action of photoinactivation on keratinocytes in vitro. In cytotoxicity assay, Keratinocytes cells were incubated with concentrations of P-123-Hyp (0.09 - 50 μmol/L) for 2 h in the dark at 37ᐤC in 0.5% CO2, subsequently the cells were irradiated with LED at 27 J/cm2 . Then, post-irradiation keratinocytes were re-incubated for 24h in the dark at 37ᐤC in 0.5% CO2. The cell viability were evaluated with MTS, compared to untreated control. The results demonstrated non-cytotoxic in cells at concentrations ranging 0.78 - 50 μmol/L, and slight cytotoxicity in concentrations 0.09 - 0.39 μmol/L of P-123-Hyp. Thus, this study showed that the use of P-123-Hyp-PDI is safe promising treatment to several diseases.
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spelling CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINEKeratinocytesPhotodynamic inactivationP-123-hypericinPhotodynamic inactivation (PDI) is a non-invasive technology, recognized since 1900, that uses a combination of a light source and a photosensitizer (PS), causing photochemical reactions. Hypericin (Hyp) is a napthodianthrone naturally isolated compound, with promising photodynamic properties by high penetration and absorption. A novel formulation was developed the encapsulated Hyp in P-123 copolymeric micelles (P123-Hyp) formed at low copolymeric concentration, with the intention to improve the photodynamic characteristics of Hyp. Therefore, its necessary evaluate if PDI mediated P-123-Hyp cause cytotoxicity in cell line human. For this, assay was performed to evaluate the action of photoinactivation on keratinocytes in vitro. In cytotoxicity assay, Keratinocytes cells were incubated with concentrations of P-123-Hyp (0.09 - 50 μmol/L) for 2 h in the dark at 37ᐤC in 0.5% CO2, subsequently the cells were irradiated with LED at 27 J/cm2 . Then, post-irradiation keratinocytes were re-incubated for 24h in the dark at 37ᐤC in 0.5% CO2. The cell viability were evaluated with MTS, compared to untreated control. The results demonstrated non-cytotoxic in cells at concentrations ranging 0.78 - 50 μmol/L, and slight cytotoxicity in concentrations 0.09 - 0.39 μmol/L of P-123-Hyp. Thus, this study showed that the use of P-123-Hyp-PDI is safe promising treatment to several diseases.UniCesumar2019-12-30T18:32:18Z2019-12-30T18:32:18Z2019-10-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf978-85-459-1960-52594-4991http://rdu.unicesumar.edu.br//handle/123456789/3677otherCONRADO, Pollyanna Cristina VincenziSAKITA, Karina MayumiARITA, Glaucia SayuriGALINARI, Camila BarrosBONFIM-MENDONÇA, Patrícia de SouzaKIOSHIMA, Erika Sekireponame:Repositório Digital Unicesumarinstname:Centro Universitário de Maringáinstacron:UniCesumarinfo:eu-repo/semantics/openAccess2020-06-13T17:10:16Zhttp://rdu.unicesumar.edu.br/PRIhttp://rdu.unicesumar.edu.br/oai/requestjoao.souza@unicesumar.edu.bropendoar:2020-06-13 17:10:21.479Repositório Digital Unicesumar - Centro Universitário de Maringáfalse
dc.title.none.fl_str_mv CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
title CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
spellingShingle CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
CONRADO, Pollyanna Cristina Vincenzi
Keratinocytes
Photodynamic inactivation
P-123-hypericin
title_short CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
title_full CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
title_fullStr CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
title_full_unstemmed CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
title_sort CYTOTOXICITY OF P-123-HYPERICIN PHOTODYNAMIC INACTIVATION ON HUMAN CELLS LINE
author CONRADO, Pollyanna Cristina Vincenzi
author_facet CONRADO, Pollyanna Cristina Vincenzi
SAKITA, Karina Mayumi
ARITA, Glaucia Sayuri
GALINARI, Camila Barros
BONFIM-MENDONÇA, Patrícia de Souza
KIOSHIMA, Erika Seki
author_role author
author2 SAKITA, Karina Mayumi
ARITA, Glaucia Sayuri
GALINARI, Camila Barros
BONFIM-MENDONÇA, Patrícia de Souza
KIOSHIMA, Erika Seki
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv CONRADO, Pollyanna Cristina Vincenzi
SAKITA, Karina Mayumi
ARITA, Glaucia Sayuri
GALINARI, Camila Barros
BONFIM-MENDONÇA, Patrícia de Souza
KIOSHIMA, Erika Seki
dc.subject.por.fl_str_mv Keratinocytes
Photodynamic inactivation
P-123-hypericin
topic Keratinocytes
Photodynamic inactivation
P-123-hypericin
dc.description.none.fl_txt_mv Photodynamic inactivation (PDI) is a non-invasive technology, recognized since 1900, that uses a combination of a light source and a photosensitizer (PS), causing photochemical reactions. Hypericin (Hyp) is a napthodianthrone naturally isolated compound, with promising photodynamic properties by high penetration and absorption. A novel formulation was developed the encapsulated Hyp in P-123 copolymeric micelles (P123-Hyp) formed at low copolymeric concentration, with the intention to improve the photodynamic characteristics of Hyp. Therefore, its necessary evaluate if PDI mediated P-123-Hyp cause cytotoxicity in cell line human. For this, assay was performed to evaluate the action of photoinactivation on keratinocytes in vitro. In cytotoxicity assay, Keratinocytes cells were incubated with concentrations of P-123-Hyp (0.09 - 50 μmol/L) for 2 h in the dark at 37ᐤC in 0.5% CO2, subsequently the cells were irradiated with LED at 27 J/cm2 . Then, post-irradiation keratinocytes were re-incubated for 24h in the dark at 37ᐤC in 0.5% CO2. The cell viability were evaluated with MTS, compared to untreated control. The results demonstrated non-cytotoxic in cells at concentrations ranging 0.78 - 50 μmol/L, and slight cytotoxicity in concentrations 0.09 - 0.39 μmol/L of P-123-Hyp. Thus, this study showed that the use of P-123-Hyp-PDI is safe promising treatment to several diseases.
description Photodynamic inactivation (PDI) is a non-invasive technology, recognized since 1900, that uses a combination of a light source and a photosensitizer (PS), causing photochemical reactions. Hypericin (Hyp) is a napthodianthrone naturally isolated compound, with promising photodynamic properties by high penetration and absorption. A novel formulation was developed the encapsulated Hyp in P-123 copolymeric micelles (P123-Hyp) formed at low copolymeric concentration, with the intention to improve the photodynamic characteristics of Hyp. Therefore, its necessary evaluate if PDI mediated P-123-Hyp cause cytotoxicity in cell line human. For this, assay was performed to evaluate the action of photoinactivation on keratinocytes in vitro. In cytotoxicity assay, Keratinocytes cells were incubated with concentrations of P-123-Hyp (0.09 - 50 μmol/L) for 2 h in the dark at 37ᐤC in 0.5% CO2, subsequently the cells were irradiated with LED at 27 J/cm2 . Then, post-irradiation keratinocytes were re-incubated for 24h in the dark at 37ᐤC in 0.5% CO2. The cell viability were evaluated with MTS, compared to untreated control. The results demonstrated non-cytotoxic in cells at concentrations ranging 0.78 - 50 μmol/L, and slight cytotoxicity in concentrations 0.09 - 0.39 μmol/L of P-123-Hyp. Thus, this study showed that the use of P-123-Hyp-PDI is safe promising treatment to several diseases.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-30T18:32:18Z
2019-12-30T18:32:18Z
2019-10-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
status_str publishedVersion
format article
dc.identifier.uri.fl_str_mv 978-85-459-1960-5
2594-4991
http://rdu.unicesumar.edu.br//handle/123456789/3677
identifier_str_mv 978-85-459-1960-5
2594-4991
url http://rdu.unicesumar.edu.br//handle/123456789/3677
dc.language.iso.fl_str_mv other
language_invalid_str_mv other
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UniCesumar
publisher.none.fl_str_mv UniCesumar
dc.source.none.fl_str_mv reponame:Repositório Digital Unicesumar
instname:Centro Universitário de Maringá
instacron:UniCesumar
reponame_str Repositório Digital Unicesumar
collection Repositório Digital Unicesumar
instname_str Centro Universitário de Maringá
instacron_str UniCesumar
institution UniCesumar
repository.name.fl_str_mv Repositório Digital Unicesumar - Centro Universitário de Maringá
repository.mail.fl_str_mv joao.souza@unicesumar.edu.br
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