Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/plants9040525 http://hdl.handle.net/11449/198795 |
Resumo: | Terpenoids are a diverse class of metabolites that impact plant metabolism in response to environmental cues. They are synthesized either via a predominantly cytosolic (MVA) pathway or a plastidic pathway (MEP). In Arabidopsis, several enzymes from the MVA and MEP pathways are encoded by gene families, excluding MVK and DXR, which are single-copy genes. In this study, we assess the diversity, evolution and expression of DXR and MVK genes in selected angiosperms and Coffea arabica in particular. Evolutionary analysis revealed that DXR and MVK underwent purifying selection, but the selection effect for DXR was stronger than it was for MVK. Digital gene expression (DGE) profile analysis of six species revealed that expression levels of MVK in flowers and roots were high, whereas for DXR peak values were observed in leaves. In C. arabica, both genes were highly expressed in flowers, and CaDXR was upregulated in response to methyl jasmonate. C. arabica DGE data were validated by assessing gene expression in selected organs, and by plants treated with hexanoic acid (Hx) using RT-qPCR. MVK expression was upregulated in roots treated with Hx. CaDXR was downregulated in leaves by Hx treatment in a genotype-specific manner, indicating a differential response to priming. |
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Repositório Institucional da UNESP |
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Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiospermsCoffeaMVA and MEP pathwaysPurifying selectionRT-qPCRTerpenoids are a diverse class of metabolites that impact plant metabolism in response to environmental cues. They are synthesized either via a predominantly cytosolic (MVA) pathway or a plastidic pathway (MEP). In Arabidopsis, several enzymes from the MVA and MEP pathways are encoded by gene families, excluding MVK and DXR, which are single-copy genes. In this study, we assess the diversity, evolution and expression of DXR and MVK genes in selected angiosperms and Coffea arabica in particular. Evolutionary analysis revealed that DXR and MVK underwent purifying selection, but the selection effect for DXR was stronger than it was for MVK. Digital gene expression (DGE) profile analysis of six species revealed that expression levels of MVK in flowers and roots were high, whereas for DXR peak values were observed in leaves. In C. arabica, both genes were highly expressed in flowers, and CaDXR was upregulated in response to methyl jasmonate. C. arabica DGE data were validated by assessing gene expression in selected organs, and by plants treated with hexanoic acid (Hx) using RT-qPCR. MVK expression was upregulated in roots treated with Hx. CaDXR was downregulated in leaves by Hx treatment in a genotype-specific manner, indicating a differential response to priming.Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESPLaboratório de Biotecnologia Vegetal Empresa Brasileira de Pesquisa Agropecuária (Embrapa-Café)Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESPUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Silva, Natacha [UNESP]Ivamoto-Suzuki, Suzana Tiemi [UNESP]Camargo, Paula Oliveira [UNESP]Rosa, Raíssa Scalzoni [UNESP]Pereira, Luiz Filipe ProtasioDomingues, Douglas Silva [UNESP]2020-12-12T01:22:14Z2020-12-12T01:22:14Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/plants9040525Plants, v. 9, n. 4, 2020.2223-7747http://hdl.handle.net/11449/19879510.3390/plants90405252-s2.0-85084214060Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlantsinfo:eu-repo/semantics/openAccess2021-10-22T20:28:32Zoai:repositorio.unesp.br:11449/198795Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:28:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
title |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
spellingShingle |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms Silva, Natacha [UNESP] Coffea MVA and MEP pathways Purifying selection RT-qPCR |
title_short |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
title_full |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
title_fullStr |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
title_full_unstemmed |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
title_sort |
Low-copy genes in terpenoid metabolism: The evolution and expression of MVK and DXR genes in angiosperms |
author |
Silva, Natacha [UNESP] |
author_facet |
Silva, Natacha [UNESP] Ivamoto-Suzuki, Suzana Tiemi [UNESP] Camargo, Paula Oliveira [UNESP] Rosa, Raíssa Scalzoni [UNESP] Pereira, Luiz Filipe Protasio Domingues, Douglas Silva [UNESP] |
author_role |
author |
author2 |
Ivamoto-Suzuki, Suzana Tiemi [UNESP] Camargo, Paula Oliveira [UNESP] Rosa, Raíssa Scalzoni [UNESP] Pereira, Luiz Filipe Protasio Domingues, Douglas Silva [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Silva, Natacha [UNESP] Ivamoto-Suzuki, Suzana Tiemi [UNESP] Camargo, Paula Oliveira [UNESP] Rosa, Raíssa Scalzoni [UNESP] Pereira, Luiz Filipe Protasio Domingues, Douglas Silva [UNESP] |
dc.subject.por.fl_str_mv |
Coffea MVA and MEP pathways Purifying selection RT-qPCR |
topic |
Coffea MVA and MEP pathways Purifying selection RT-qPCR |
description |
Terpenoids are a diverse class of metabolites that impact plant metabolism in response to environmental cues. They are synthesized either via a predominantly cytosolic (MVA) pathway or a plastidic pathway (MEP). In Arabidopsis, several enzymes from the MVA and MEP pathways are encoded by gene families, excluding MVK and DXR, which are single-copy genes. In this study, we assess the diversity, evolution and expression of DXR and MVK genes in selected angiosperms and Coffea arabica in particular. Evolutionary analysis revealed that DXR and MVK underwent purifying selection, but the selection effect for DXR was stronger than it was for MVK. Digital gene expression (DGE) profile analysis of six species revealed that expression levels of MVK in flowers and roots were high, whereas for DXR peak values were observed in leaves. In C. arabica, both genes were highly expressed in flowers, and CaDXR was upregulated in response to methyl jasmonate. C. arabica DGE data were validated by assessing gene expression in selected organs, and by plants treated with hexanoic acid (Hx) using RT-qPCR. MVK expression was upregulated in roots treated with Hx. CaDXR was downregulated in leaves by Hx treatment in a genotype-specific manner, indicating a differential response to priming. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:22:14Z 2020-12-12T01:22:14Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/plants9040525 Plants, v. 9, n. 4, 2020. 2223-7747 http://hdl.handle.net/11449/198795 10.3390/plants9040525 2-s2.0-85084214060 |
url |
http://dx.doi.org/10.3390/plants9040525 http://hdl.handle.net/11449/198795 |
identifier_str_mv |
Plants, v. 9, n. 4, 2020. 2223-7747 10.3390/plants9040525 2-s2.0-85084214060 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789660988047360 |