Characterization of a monoclonal antibody recognizing mast cells
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/230896 |
Resumo: | Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells. |
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Characterization of a monoclonal antibody recognizing mast cellsConnective tissueHeterogeneityImmunohistochemistryIntestineMast cellsImmunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells.School of Dentistry of São José dos Campos UNESP, São PauloDepartment of Anatomy and Cell Biology Faculty of Medicine University of Toronto, Toronto, Ont.Faculdade de Odontologia de São José dos Campos Departmento de Patologia, Av. José Longo, 777, CEP 12245 Sao Jose dos Campos, SPSchool of Dentistry of São José dos Campos UNESP, São PauloUniversidade Estadual Paulista (UNESP)University of TorontoFaculdade de Odontologia de São José dos CamposDe Oliveira Nogueira, T. [UNESP]Aubin, J. E.2022-04-29T08:42:30Z2022-04-29T08:42:30Z1997-10-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article919-923Histology and Histopathology, v. 12, n. 4, p. 919-923, 1997.0213-3911http://hdl.handle.net/11449/2308962-s2.0-0030861549Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology and Histopathologyinfo:eu-repo/semantics/openAccess2022-04-29T08:42:30Zoai:repositorio.unesp.br:11449/230896Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:37:39.537300Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of a monoclonal antibody recognizing mast cells |
title |
Characterization of a monoclonal antibody recognizing mast cells |
spellingShingle |
Characterization of a monoclonal antibody recognizing mast cells De Oliveira Nogueira, T. [UNESP] Connective tissue Heterogeneity Immunohistochemistry Intestine Mast cells |
title_short |
Characterization of a monoclonal antibody recognizing mast cells |
title_full |
Characterization of a monoclonal antibody recognizing mast cells |
title_fullStr |
Characterization of a monoclonal antibody recognizing mast cells |
title_full_unstemmed |
Characterization of a monoclonal antibody recognizing mast cells |
title_sort |
Characterization of a monoclonal antibody recognizing mast cells |
author |
De Oliveira Nogueira, T. [UNESP] |
author_facet |
De Oliveira Nogueira, T. [UNESP] Aubin, J. E. |
author_role |
author |
author2 |
Aubin, J. E. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Toronto Faculdade de Odontologia de São José dos Campos |
dc.contributor.author.fl_str_mv |
De Oliveira Nogueira, T. [UNESP] Aubin, J. E. |
dc.subject.por.fl_str_mv |
Connective tissue Heterogeneity Immunohistochemistry Intestine Mast cells |
topic |
Connective tissue Heterogeneity Immunohistochemistry Intestine Mast cells |
description |
Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-10-08 2022-04-29T08:42:30Z 2022-04-29T08:42:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Histology and Histopathology, v. 12, n. 4, p. 919-923, 1997. 0213-3911 http://hdl.handle.net/11449/230896 2-s2.0-0030861549 |
identifier_str_mv |
Histology and Histopathology, v. 12, n. 4, p. 919-923, 1997. 0213-3911 2-s2.0-0030861549 |
url |
http://hdl.handle.net/11449/230896 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Histology and Histopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
919-923 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128390157303808 |