Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2017.05.007 http://hdl.handle.net/11449/174570 |
Resumo: | Ten normal prostates, 22 benign prostatic hyperplasia (BPH) and 29 prostate cancer (PC) were morphometrically analyzed with regard to mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear diameter (MND), coefficient of variation of the nuclear area (NACV), mean nuclear diameter maximum (MDx), mean nuclear diameter minimum (MDm), mean nuclear form ellipse (MNFe) and form factor (FF). The relationship between nuclear morphometric parameters and histological type, Gleason score, methods of sample collection, presence of metastases and survival time of canine PC were also investigated. Overall, nuclei from neoplastic cells were larger, with greater variation in nuclear size and shape compared to normal and hyperplastic cells. Significant differences were found between more (small acinar/ductal) and less (cribriform, solid) differentiated PCs with regard to FF (p < 0.05). MNA, MNP, MND, MDx, and MDm were significantly correlated with the Gleason score of PC (p < 0.05). MNA, MNP, MDx and MNFe may also have important prognostic implications in canine prostatic cancer since negatively correlated with the survival time. Biopsy specimens contained nuclei that were smaller and more irregular in comparison to those in prostatectomy and necropsy specimens and therefore factors associated with tissue sampling and processing may influence the overall morphometric evaluation. The results indicate that nuclear morphometric analysis in combination with Gleason score can help in canine prostate cancer grading, thus contributing to the establishment of a more precise prognosis and patient's management. |
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Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival timeCancerDogNuclear morphometryProstateTen normal prostates, 22 benign prostatic hyperplasia (BPH) and 29 prostate cancer (PC) were morphometrically analyzed with regard to mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear diameter (MND), coefficient of variation of the nuclear area (NACV), mean nuclear diameter maximum (MDx), mean nuclear diameter minimum (MDm), mean nuclear form ellipse (MNFe) and form factor (FF). The relationship between nuclear morphometric parameters and histological type, Gleason score, methods of sample collection, presence of metastases and survival time of canine PC were also investigated. Overall, nuclei from neoplastic cells were larger, with greater variation in nuclear size and shape compared to normal and hyperplastic cells. Significant differences were found between more (small acinar/ductal) and less (cribriform, solid) differentiated PCs with regard to FF (p < 0.05). MNA, MNP, MND, MDx, and MDm were significantly correlated with the Gleason score of PC (p < 0.05). MNA, MNP, MDx and MNFe may also have important prognostic implications in canine prostatic cancer since negatively correlated with the survival time. Biopsy specimens contained nuclei that were smaller and more irregular in comparison to those in prostatectomy and necropsy specimens and therefore factors associated with tissue sampling and processing may influence the overall morphometric evaluation. The results indicate that nuclear morphometric analysis in combination with Gleason score can help in canine prostate cancer grading, thus contributing to the establishment of a more precise prognosis and patient's management.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise “G. Caporale”Department of Veterinary Clinic School of Veterinary Medicine and Animal Science – Univ. Estadual Paulista – UNESPSchool of Veterinary Science The University of Queensland, Gatton campusDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science – Univ. Estadual Paulista – UNESPCNPq: 306055/2011-2012CNPq: 443884/2014-2015Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise “G. Caporale”Universidade Estadual Paulista (Unesp)The University of QueenslandDi Donato, GuidoLaufer-Amorim, Renée [UNESP]Palmieri, Chiara2018-12-11T17:11:45Z2018-12-11T17:11:45Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article212-217application/pdfhttp://dx.doi.org/10.1016/j.rvsc.2017.05.007Research in Veterinary Science, v. 114, p. 212-217.1532-26610034-5288http://hdl.handle.net/11449/17457010.1016/j.rvsc.2017.05.0072-s2.0-850191390442-s2.0-85019139044.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Science0,593info:eu-repo/semantics/openAccess2023-11-30T06:15:27Zoai:repositorio.unesp.br:11449/174570Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:08:35.707720Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
title |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
spellingShingle |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time Di Donato, Guido Cancer Dog Nuclear morphometry Prostate |
title_short |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
title_full |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
title_fullStr |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
title_full_unstemmed |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
title_sort |
Nuclear morphometry in histological specimens of canine prostate cancer: Correlation with histological subtypes, Gleason score, methods of collection and survival time |
author |
Di Donato, Guido |
author_facet |
Di Donato, Guido Laufer-Amorim, Renée [UNESP] Palmieri, Chiara |
author_role |
author |
author2 |
Laufer-Amorim, Renée [UNESP] Palmieri, Chiara |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise “G. Caporale” Universidade Estadual Paulista (Unesp) The University of Queensland |
dc.contributor.author.fl_str_mv |
Di Donato, Guido Laufer-Amorim, Renée [UNESP] Palmieri, Chiara |
dc.subject.por.fl_str_mv |
Cancer Dog Nuclear morphometry Prostate |
topic |
Cancer Dog Nuclear morphometry Prostate |
description |
Ten normal prostates, 22 benign prostatic hyperplasia (BPH) and 29 prostate cancer (PC) were morphometrically analyzed with regard to mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear diameter (MND), coefficient of variation of the nuclear area (NACV), mean nuclear diameter maximum (MDx), mean nuclear diameter minimum (MDm), mean nuclear form ellipse (MNFe) and form factor (FF). The relationship between nuclear morphometric parameters and histological type, Gleason score, methods of sample collection, presence of metastases and survival time of canine PC were also investigated. Overall, nuclei from neoplastic cells were larger, with greater variation in nuclear size and shape compared to normal and hyperplastic cells. Significant differences were found between more (small acinar/ductal) and less (cribriform, solid) differentiated PCs with regard to FF (p < 0.05). MNA, MNP, MND, MDx, and MDm were significantly correlated with the Gleason score of PC (p < 0.05). MNA, MNP, MDx and MNFe may also have important prognostic implications in canine prostatic cancer since negatively correlated with the survival time. Biopsy specimens contained nuclei that were smaller and more irregular in comparison to those in prostatectomy and necropsy specimens and therefore factors associated with tissue sampling and processing may influence the overall morphometric evaluation. The results indicate that nuclear morphometric analysis in combination with Gleason score can help in canine prostate cancer grading, thus contributing to the establishment of a more precise prognosis and patient's management. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T17:11:45Z 2018-12-11T17:11:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2017.05.007 Research in Veterinary Science, v. 114, p. 212-217. 1532-2661 0034-5288 http://hdl.handle.net/11449/174570 10.1016/j.rvsc.2017.05.007 2-s2.0-85019139044 2-s2.0-85019139044.pdf |
url |
http://dx.doi.org/10.1016/j.rvsc.2017.05.007 http://hdl.handle.net/11449/174570 |
identifier_str_mv |
Research in Veterinary Science, v. 114, p. 212-217. 1532-2661 0034-5288 10.1016/j.rvsc.2017.05.007 2-s2.0-85019139044 2-s2.0-85019139044.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science 0,593 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
212-217 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129024219676672 |