A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2022.105539 http://hdl.handle.net/11449/240910 |
Resumo: | Sporotrichosis is a cosmopolitan mycosis caused by pathogenic species of Sporothrix genus, that in Brazil is often acquired by zoonotic transmission involved infected cats with S. brasiliensis. Previous studies showed that the Sporothrix spp. recombinant enolase (rSsEno), a multifunctional protein with immunogenic properties, could be a promising target for vaccination against sporotrichosis in cats. Nevertheless, the considerable sequence identity (62%) of SsEno with its feline counterpart is a great concern. Here, we report the identification in silico, chemical synthesis and biological validation of six peptides of SsEno with low sequence identity to its cat orthologue. All synthesized peptides exhibit B-cell epitopes on the molecular surface of SsEno and proved to be highly reactive with the serum of infected mice with S. brasiliensis and sera of cats with sporotrichosis. Interestingly, our study revealed that anti-peptide sera did not react with the recombinant enolase from Felis catus (cats, rFcEno), thus, may not trigger autoimmune response in these felines if used as a vaccine antigen. The immunization with peptide mixture (PeptMix) formulated with Freund adjuvant (FA), induced high levels of antigen-specific IgG, IgG1 and IgG2b antibodies that conferred protection upon passive transference in infected BALB/c mice with S. brasiliensis. We also observed, that the FA+PeptMix formulation induced a Th1/Th2/Th17 cytokine profile ex vivo, associated with protecting effect against the experimental sporotrichosis. Our results suggest that the six SsEno-derived peptides here evaluated, could be used as safe antigens for the development of vaccine strategies against feline sporotrichosis, whether prophylactic or therapeutic. |
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A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensisEnolaseFelis catusPeptide vaccineSporothrix brasiliensisSporotrichosisSporotrichosis is a cosmopolitan mycosis caused by pathogenic species of Sporothrix genus, that in Brazil is often acquired by zoonotic transmission involved infected cats with S. brasiliensis. Previous studies showed that the Sporothrix spp. recombinant enolase (rSsEno), a multifunctional protein with immunogenic properties, could be a promising target for vaccination against sporotrichosis in cats. Nevertheless, the considerable sequence identity (62%) of SsEno with its feline counterpart is a great concern. Here, we report the identification in silico, chemical synthesis and biological validation of six peptides of SsEno with low sequence identity to its cat orthologue. All synthesized peptides exhibit B-cell epitopes on the molecular surface of SsEno and proved to be highly reactive with the serum of infected mice with S. brasiliensis and sera of cats with sporotrichosis. Interestingly, our study revealed that anti-peptide sera did not react with the recombinant enolase from Felis catus (cats, rFcEno), thus, may not trigger autoimmune response in these felines if used as a vaccine antigen. The immunization with peptide mixture (PeptMix) formulated with Freund adjuvant (FA), induced high levels of antigen-specific IgG, IgG1 and IgG2b antibodies that conferred protection upon passive transference in infected BALB/c mice with S. brasiliensis. We also observed, that the FA+PeptMix formulation induced a Th1/Th2/Th17 cytokine profile ex vivo, associated with protecting effect against the experimental sporotrichosis. Our results suggest that the six SsEno-derived peptides here evaluated, could be used as safe antigens for the development of vaccine strategies against feline sporotrichosis, whether prophylactic or therapeutic.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis, SPGC01 Immunology and Allergy Group. Maimonides Biomedical Research Institute of Cordoba (IMIBIC). Reina Sofía University Hospital, IMIBIC BuildingNúcleo de Biotecnología Curauma (NBC) Pontificia Universidad Católica de Valparaíso, Campus CuraumaSão Carlos Institute of Chemistry University of São Paulo, P.O. Box 780, SPFacultad de Ingeniería Instituto de Ciencias Químicas Aplicadas Universidad Autónoma de Chile, el Llano Subercaseaux, SantiagoGrupo de Marcadores Inmunológicos Laboratorio de Genética e Inmunología Molecular Instituto de Biología Pontificia Universidad Católica de Valparaíso, Avenida Universidad #330São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis, SPFAPESP: 2015/09340–4FAPESP: 2017/13228–0FAPESP: 2017/26774–3Universidade Estadual Paulista (UNESP)GC01 Immunology and Allergy Group. Maimonides Biomedical Research Institute of Cordoba (IMIBIC). Reina Sofía University HospitalPontificia Universidad Católica de ValparaísoUniversidade de São Paulo (USP)Universidad Autónoma de ChilePortuondo, Deivys Leandro [UNESP]Batista-Duharte, Alexander [UNESP]Cardenas, ConstanzaOliveira, Carlos S. deBorges, Júlio CésarTéllez-Martínez, Damiana [UNESP]Santana, Paula AndreaGauna, AdrianaMercado, LuisCastilho, Bruna Mateus de [UNESP]Costa, Paulo [UNESP]Guzmán, FannyCarlos, Iracilda Zeppone [UNESP]2023-03-01T20:38:10Z2023-03-01T20:38:10Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.micpath.2022.105539Microbial Pathogenesis, v. 166.1096-12080882-4010http://hdl.handle.net/11449/24091010.1016/j.micpath.2022.1055392-s2.0-85129254934Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesisinfo:eu-repo/semantics/openAccess2024-06-21T15:18:45Zoai:repositorio.unesp.br:11449/240910Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-21T15:18:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
title |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
spellingShingle |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis Portuondo, Deivys Leandro [UNESP] Enolase Felis catus Peptide vaccine Sporothrix brasiliensis Sporotrichosis |
title_short |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
title_full |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
title_fullStr |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
title_full_unstemmed |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
title_sort |
A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis |
author |
Portuondo, Deivys Leandro [UNESP] |
author_facet |
Portuondo, Deivys Leandro [UNESP] Batista-Duharte, Alexander [UNESP] Cardenas, Constanza Oliveira, Carlos S. de Borges, Júlio César Téllez-Martínez, Damiana [UNESP] Santana, Paula Andrea Gauna, Adriana Mercado, Luis Castilho, Bruna Mateus de [UNESP] Costa, Paulo [UNESP] Guzmán, Fanny Carlos, Iracilda Zeppone [UNESP] |
author_role |
author |
author2 |
Batista-Duharte, Alexander [UNESP] Cardenas, Constanza Oliveira, Carlos S. de Borges, Júlio César Téllez-Martínez, Damiana [UNESP] Santana, Paula Andrea Gauna, Adriana Mercado, Luis Castilho, Bruna Mateus de [UNESP] Costa, Paulo [UNESP] Guzmán, Fanny Carlos, Iracilda Zeppone [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) GC01 Immunology and Allergy Group. Maimonides Biomedical Research Institute of Cordoba (IMIBIC). Reina Sofía University Hospital Pontificia Universidad Católica de Valparaíso Universidade de São Paulo (USP) Universidad Autónoma de Chile |
dc.contributor.author.fl_str_mv |
Portuondo, Deivys Leandro [UNESP] Batista-Duharte, Alexander [UNESP] Cardenas, Constanza Oliveira, Carlos S. de Borges, Júlio César Téllez-Martínez, Damiana [UNESP] Santana, Paula Andrea Gauna, Adriana Mercado, Luis Castilho, Bruna Mateus de [UNESP] Costa, Paulo [UNESP] Guzmán, Fanny Carlos, Iracilda Zeppone [UNESP] |
dc.subject.por.fl_str_mv |
Enolase Felis catus Peptide vaccine Sporothrix brasiliensis Sporotrichosis |
topic |
Enolase Felis catus Peptide vaccine Sporothrix brasiliensis Sporotrichosis |
description |
Sporotrichosis is a cosmopolitan mycosis caused by pathogenic species of Sporothrix genus, that in Brazil is often acquired by zoonotic transmission involved infected cats with S. brasiliensis. Previous studies showed that the Sporothrix spp. recombinant enolase (rSsEno), a multifunctional protein with immunogenic properties, could be a promising target for vaccination against sporotrichosis in cats. Nevertheless, the considerable sequence identity (62%) of SsEno with its feline counterpart is a great concern. Here, we report the identification in silico, chemical synthesis and biological validation of six peptides of SsEno with low sequence identity to its cat orthologue. All synthesized peptides exhibit B-cell epitopes on the molecular surface of SsEno and proved to be highly reactive with the serum of infected mice with S. brasiliensis and sera of cats with sporotrichosis. Interestingly, our study revealed that anti-peptide sera did not react with the recombinant enolase from Felis catus (cats, rFcEno), thus, may not trigger autoimmune response in these felines if used as a vaccine antigen. The immunization with peptide mixture (PeptMix) formulated with Freund adjuvant (FA), induced high levels of antigen-specific IgG, IgG1 and IgG2b antibodies that conferred protection upon passive transference in infected BALB/c mice with S. brasiliensis. We also observed, that the FA+PeptMix formulation induced a Th1/Th2/Th17 cytokine profile ex vivo, associated with protecting effect against the experimental sporotrichosis. Our results suggest that the six SsEno-derived peptides here evaluated, could be used as safe antigens for the development of vaccine strategies against feline sporotrichosis, whether prophylactic or therapeutic. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01 2023-03-01T20:38:10Z 2023-03-01T20:38:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2022.105539 Microbial Pathogenesis, v. 166. 1096-1208 0882-4010 http://hdl.handle.net/11449/240910 10.1016/j.micpath.2022.105539 2-s2.0-85129254934 |
url |
http://dx.doi.org/10.1016/j.micpath.2022.105539 http://hdl.handle.net/11449/240910 |
identifier_str_mv |
Microbial Pathogenesis, v. 166. 1096-1208 0882-4010 10.1016/j.micpath.2022.105539 2-s2.0-85129254934 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649630055956480 |