Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0256199 http://hdl.handle.net/11449/222229 |
Resumo: | This study investigated the occurrence of Giardia duodenalis and Cryptosporidium spp. in rodents and marsupials from the Atlantic Forest in southern Bahia, northeastern Brazil. Two hundred and four fecal samples were collected from different forest areas in the municipalities of Ilhéus, Una, Belmonte, and Mascote. Identifications were performed using PCR and nested PCR followed by sequencing of the gdh and tpi genes for G. duodenalis, and the gp60 and Hsp-70 genes for Cryptosporidium. The total frequency of positive PCR samples for both G. duodenalis and Cryptosporidium spp. was 5.4% (11/204). Giardia duodenalis occurred in 2.94% (4/136) of rodents and 2.94% (2/68) of marsupials. The prevalence of Cryptosporidium in rodents and marsupials was 1.47% (2/136) and 4.41% (3/68), respectively. In the areas sampled, the frequency of parasitism was 50% (7/14), while the Mascote region alone had no parasitized animals. The G. duodenalis subgenotype AI was identified in the rodent species Hylaeamys laticeps, Oecomys catherinae, Oligoryzomys nigripes and Akodon cursor, and in the marsupials Gracilinanus agilis and Monodelphis americana. In the rodents Rhipidomys mastacalis, H. laticeps and in the marsupial Marmosa murina the protozoa Cryptosporidium fayeri, Cryptosporidium parvum and Cryptosporidium ubiquitum with subtypes IIa and IVg by the gp60 gene were found. In conclusion, this study provides the genetic characterization of Giardia and Cryptosporidium species and genotypes in rodents and marsupials. And, these findings reinforce that the rodent and marsupial species mentioned above play a role as new hosts for Giardia and Cryptosporidium. |
id |
UNSP_9b17e26a169ae1dd17a51f60c7b373d3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/222229 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern BrazilThis study investigated the occurrence of Giardia duodenalis and Cryptosporidium spp. in rodents and marsupials from the Atlantic Forest in southern Bahia, northeastern Brazil. Two hundred and four fecal samples were collected from different forest areas in the municipalities of Ilhéus, Una, Belmonte, and Mascote. Identifications were performed using PCR and nested PCR followed by sequencing of the gdh and tpi genes for G. duodenalis, and the gp60 and Hsp-70 genes for Cryptosporidium. The total frequency of positive PCR samples for both G. duodenalis and Cryptosporidium spp. was 5.4% (11/204). Giardia duodenalis occurred in 2.94% (4/136) of rodents and 2.94% (2/68) of marsupials. The prevalence of Cryptosporidium in rodents and marsupials was 1.47% (2/136) and 4.41% (3/68), respectively. In the areas sampled, the frequency of parasitism was 50% (7/14), while the Mascote region alone had no parasitized animals. The G. duodenalis subgenotype AI was identified in the rodent species Hylaeamys laticeps, Oecomys catherinae, Oligoryzomys nigripes and Akodon cursor, and in the marsupials Gracilinanus agilis and Monodelphis americana. In the rodents Rhipidomys mastacalis, H. laticeps and in the marsupial Marmosa murina the protozoa Cryptosporidium fayeri, Cryptosporidium parvum and Cryptosporidium ubiquitum with subtypes IIa and IVg by the gp60 gene were found. In conclusion, this study provides the genetic characterization of Giardia and Cryptosporidium species and genotypes in rodents and marsupials. And, these findings reinforce that the rodent and marsupial species mentioned above play a role as new hosts for Giardia and Cryptosporidium.Department of Agricultural and Environmental Sciences Santa Cruz State University—UESC, BADepartment of Support Production and Animal Health Universidade Estadual Paulista—UNESP, SPDepartment of Biological Sciences State University of Santa Cruz—UESC, BADepartamento de Ciências Agrárias e Ambientais Hospital Veterinário Universidade Estadual de Santa Cruz, Ilhéus, BahiaDepartment of Support Production and Animal Health Universidade Estadual Paulista—UNESP, SPSanta Cruz State University—UESCUniversidade Estadual Paulista (UNESP)State University of Santa Cruz—UESCUniversidade Estadual de Santa CruzFehlberg, Hllytchaikra FerrazRibeiro, Cássia Matosde Alcântara Brito Junior, PedroOliveira, Bruno César Miranda [UNESP]dos Santos, Camila Albanodel Valle Alvarez, Martín RobertoHarvey, Tatiane VitorAlbuquerque, George Rêgo2022-04-28T19:43:30Z2022-04-28T19:43:30Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0256199PLoS ONE, v. 16, n. 8 August, 2021.1932-6203http://hdl.handle.net/11449/22222910.1371/journal.pone.02561992-s2.0-85112739402Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2022-04-28T19:43:30Zoai:repositorio.unesp.br:11449/222229Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:17:48.779797Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
title |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
spellingShingle |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil Fehlberg, Hllytchaikra Ferraz |
title_short |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
title_full |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
title_fullStr |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
title_full_unstemmed |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
title_sort |
Detection of Cryptosporidium spp. And Giardia duodenalis in small wild mammals in northeastern Brazil |
author |
Fehlberg, Hllytchaikra Ferraz |
author_facet |
Fehlberg, Hllytchaikra Ferraz Ribeiro, Cássia Matos de Alcântara Brito Junior, Pedro Oliveira, Bruno César Miranda [UNESP] dos Santos, Camila Albano del Valle Alvarez, Martín Roberto Harvey, Tatiane Vitor Albuquerque, George Rêgo |
author_role |
author |
author2 |
Ribeiro, Cássia Matos de Alcântara Brito Junior, Pedro Oliveira, Bruno César Miranda [UNESP] dos Santos, Camila Albano del Valle Alvarez, Martín Roberto Harvey, Tatiane Vitor Albuquerque, George Rêgo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Santa Cruz State University—UESC Universidade Estadual Paulista (UNESP) State University of Santa Cruz—UESC Universidade Estadual de Santa Cruz |
dc.contributor.author.fl_str_mv |
Fehlberg, Hllytchaikra Ferraz Ribeiro, Cássia Matos de Alcântara Brito Junior, Pedro Oliveira, Bruno César Miranda [UNESP] dos Santos, Camila Albano del Valle Alvarez, Martín Roberto Harvey, Tatiane Vitor Albuquerque, George Rêgo |
description |
This study investigated the occurrence of Giardia duodenalis and Cryptosporidium spp. in rodents and marsupials from the Atlantic Forest in southern Bahia, northeastern Brazil. Two hundred and four fecal samples were collected from different forest areas in the municipalities of Ilhéus, Una, Belmonte, and Mascote. Identifications were performed using PCR and nested PCR followed by sequencing of the gdh and tpi genes for G. duodenalis, and the gp60 and Hsp-70 genes for Cryptosporidium. The total frequency of positive PCR samples for both G. duodenalis and Cryptosporidium spp. was 5.4% (11/204). Giardia duodenalis occurred in 2.94% (4/136) of rodents and 2.94% (2/68) of marsupials. The prevalence of Cryptosporidium in rodents and marsupials was 1.47% (2/136) and 4.41% (3/68), respectively. In the areas sampled, the frequency of parasitism was 50% (7/14), while the Mascote region alone had no parasitized animals. The G. duodenalis subgenotype AI was identified in the rodent species Hylaeamys laticeps, Oecomys catherinae, Oligoryzomys nigripes and Akodon cursor, and in the marsupials Gracilinanus agilis and Monodelphis americana. In the rodents Rhipidomys mastacalis, H. laticeps and in the marsupial Marmosa murina the protozoa Cryptosporidium fayeri, Cryptosporidium parvum and Cryptosporidium ubiquitum with subtypes IIa and IVg by the gp60 gene were found. In conclusion, this study provides the genetic characterization of Giardia and Cryptosporidium species and genotypes in rodents and marsupials. And, these findings reinforce that the rodent and marsupial species mentioned above play a role as new hosts for Giardia and Cryptosporidium. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-04-28T19:43:30Z 2022-04-28T19:43:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0256199 PLoS ONE, v. 16, n. 8 August, 2021. 1932-6203 http://hdl.handle.net/11449/222229 10.1371/journal.pone.0256199 2-s2.0-85112739402 |
url |
http://dx.doi.org/10.1371/journal.pone.0256199 http://hdl.handle.net/11449/222229 |
identifier_str_mv |
PLoS ONE, v. 16, n. 8 August, 2021. 1932-6203 10.1371/journal.pone.0256199 2-s2.0-85112739402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129504638402560 |