Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state

Detalhes bibliográficos
Autor(a) principal: Mourao, Aline A.
Data de Publicação: 2018
Outros Autores: Soares de Mello, Aryanne B., Dos Santos Moreira, Marina C., Rodrigues, Karla L., Lopes, Paulo R., Xavier, Carlos H., Gomes, Rodrigo M., Freiria-Oliveira, Andre H., Blanch, Graziela T., Colombari, Eduardo [UNESP], Pedrino, Gustavo R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.brainresbull.2018.06.011
http://hdl.handle.net/11449/184983
Resumo: The crucial role of the median preoptic nucleus (MnPO) in the maintenance of hydroelectrolytic balance and autonomic regulation have been highlighted. Recently, the participation of the MnPO in the control of sympathetic nerve activity was demonstrated in essential hypertension model. However, peculiarities on the neurochemical changes underlying the differential role of MnPO during hypertension remain to be clarified. Therefore, this study aimed to investigate the main excitatory pathways that modulate MnPO neurons in hypertensive rats. Spontaneously hypertensive rats (SHR) and rats submitted previously to the Goldblatt protocol (two kidneys; one clip; 2K1C) were used. Rats of both groups (250 to 350 g, n = 6) were anesthetized with urethane (1.2 g/kg,i.v.) and instrumented to record mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Nanoinjection (100 nl) of saline (NaCl, 150 mM), losartan (AT1 receptor antagonist; 10 mM) and kynurenic acid (glutamate receptor antagonist; 50 mM) into the MnPO were performed. In 2K1C rats, glutamatergic blockade promoted decreases in MAP and RSNA (-19.1 +/- 0.9 mmHg, -21.6 +/- 2.8%, p < 0.05) when compared to saline (-0.4 +/- 0.6 mmHg, 0.2 +/- 0.7%, p < 0.05). Angiotensinergic inhibition also reduced these parameters (-11.5 +/- 1.2 mmHg, -10.5 +/- 1.0%, p < 0.05) in 2K1C. In SHR, Kynurenic acid nanoinjections produced hypotension and sympathoinhibition (-21.0 +/- 2.5 mmHg, -24.7 +/- 2.4%, p < 0.05), as well losartan nanoinjections (-9.7 +/- 1.2 mmHg; p < 0.05) and RSNA (-12.0 +/- 2.4%, p < 0.05). These findings support the conclusion that a tonic excitatory neurotransmission exerted by angiotensin II, and mostly by glutamate in the MnPO could participate in the modulation of blood pressure and RSNA independent on whether hypertension is primarily neurogenic or is secondary to stenosis in renal artery.
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spelling Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive stateMnPO2K1CSHRGlutamateAngiotensin IIThe crucial role of the median preoptic nucleus (MnPO) in the maintenance of hydroelectrolytic balance and autonomic regulation have been highlighted. Recently, the participation of the MnPO in the control of sympathetic nerve activity was demonstrated in essential hypertension model. However, peculiarities on the neurochemical changes underlying the differential role of MnPO during hypertension remain to be clarified. Therefore, this study aimed to investigate the main excitatory pathways that modulate MnPO neurons in hypertensive rats. Spontaneously hypertensive rats (SHR) and rats submitted previously to the Goldblatt protocol (two kidneys; one clip; 2K1C) were used. Rats of both groups (250 to 350 g, n = 6) were anesthetized with urethane (1.2 g/kg,i.v.) and instrumented to record mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Nanoinjection (100 nl) of saline (NaCl, 150 mM), losartan (AT1 receptor antagonist; 10 mM) and kynurenic acid (glutamate receptor antagonist; 50 mM) into the MnPO were performed. In 2K1C rats, glutamatergic blockade promoted decreases in MAP and RSNA (-19.1 +/- 0.9 mmHg, -21.6 +/- 2.8%, p < 0.05) when compared to saline (-0.4 +/- 0.6 mmHg, 0.2 +/- 0.7%, p < 0.05). Angiotensinergic inhibition also reduced these parameters (-11.5 +/- 1.2 mmHg, -10.5 +/- 1.0%, p < 0.05) in 2K1C. In SHR, Kynurenic acid nanoinjections produced hypotension and sympathoinhibition (-21.0 +/- 2.5 mmHg, -24.7 +/- 2.4%, p < 0.05), as well losartan nanoinjections (-9.7 +/- 1.2 mmHg; p < 0.05) and RSNA (-12.0 +/- 2.4%, p < 0.05). These findings support the conclusion that a tonic excitatory neurotransmission exerted by angiotensin II, and mostly by glutamate in the MnPO could participate in the modulation of blood pressure and RSNA independent on whether hypertension is primarily neurogenic or is secondary to stenosis in renal artery.Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Biol Sci Inst, Dept Physiol Sci, Ctr Neurosci & Cardiovasc Res, Goiania, Go, BrazilUniv Fed Goias, Biol Sci Inst, Dept Physiol Sci, Lab Cardiovasc Physiol & Therapeut, Goiania, Go, BrazilPontifical Catholic Univ Goias, Sch Med Pharm & Biomed, Goiania, Go, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilFundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2012/0055431086Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2009/10267000352CNPq: 483411/2012-4CNPq: 447496/2014-0Elsevier B.V.Universidade Federal de Goiás (UFG)Pontifical Catholic Univ GoiasUniversidade Estadual Paulista (Unesp)Mourao, Aline A.Soares de Mello, Aryanne B.Dos Santos Moreira, Marina C.Rodrigues, Karla L.Lopes, Paulo R.Xavier, Carlos H.Gomes, Rodrigo M.Freiria-Oliveira, Andre H.Blanch, Graziela T.Colombari, Eduardo [UNESP]Pedrino, Gustavo R.2019-10-04T12:31:45Z2019-10-04T12:31:45Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article207-215http://dx.doi.org/10.1016/j.brainresbull.2018.06.011Brain Research Bulletin. Oxford: Pergamon-elsevier Science Ltd, v. 142, p. 207-215, 2018.0361-9230http://hdl.handle.net/11449/18498310.1016/j.brainresbull.2018.06.011WOS:000448223400023Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Research Bulletininfo:eu-repo/semantics/openAccess2024-09-27T14:04:57Zoai:repositorio.unesp.br:11449/184983Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:04:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
title Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
spellingShingle Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
Mourao, Aline A.
MnPO
2K1C
SHR
Glutamate
Angiotensin II
title_short Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
title_full Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
title_fullStr Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
title_full_unstemmed Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
title_sort Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
author Mourao, Aline A.
author_facet Mourao, Aline A.
Soares de Mello, Aryanne B.
Dos Santos Moreira, Marina C.
Rodrigues, Karla L.
Lopes, Paulo R.
Xavier, Carlos H.
Gomes, Rodrigo M.
Freiria-Oliveira, Andre H.
Blanch, Graziela T.
Colombari, Eduardo [UNESP]
Pedrino, Gustavo R.
author_role author
author2 Soares de Mello, Aryanne B.
Dos Santos Moreira, Marina C.
Rodrigues, Karla L.
Lopes, Paulo R.
Xavier, Carlos H.
Gomes, Rodrigo M.
Freiria-Oliveira, Andre H.
Blanch, Graziela T.
Colombari, Eduardo [UNESP]
Pedrino, Gustavo R.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Pontifical Catholic Univ Goias
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Mourao, Aline A.
Soares de Mello, Aryanne B.
Dos Santos Moreira, Marina C.
Rodrigues, Karla L.
Lopes, Paulo R.
Xavier, Carlos H.
Gomes, Rodrigo M.
Freiria-Oliveira, Andre H.
Blanch, Graziela T.
Colombari, Eduardo [UNESP]
Pedrino, Gustavo R.
dc.subject.por.fl_str_mv MnPO
2K1C
SHR
Glutamate
Angiotensin II
topic MnPO
2K1C
SHR
Glutamate
Angiotensin II
description The crucial role of the median preoptic nucleus (MnPO) in the maintenance of hydroelectrolytic balance and autonomic regulation have been highlighted. Recently, the participation of the MnPO in the control of sympathetic nerve activity was demonstrated in essential hypertension model. However, peculiarities on the neurochemical changes underlying the differential role of MnPO during hypertension remain to be clarified. Therefore, this study aimed to investigate the main excitatory pathways that modulate MnPO neurons in hypertensive rats. Spontaneously hypertensive rats (SHR) and rats submitted previously to the Goldblatt protocol (two kidneys; one clip; 2K1C) were used. Rats of both groups (250 to 350 g, n = 6) were anesthetized with urethane (1.2 g/kg,i.v.) and instrumented to record mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Nanoinjection (100 nl) of saline (NaCl, 150 mM), losartan (AT1 receptor antagonist; 10 mM) and kynurenic acid (glutamate receptor antagonist; 50 mM) into the MnPO were performed. In 2K1C rats, glutamatergic blockade promoted decreases in MAP and RSNA (-19.1 +/- 0.9 mmHg, -21.6 +/- 2.8%, p < 0.05) when compared to saline (-0.4 +/- 0.6 mmHg, 0.2 +/- 0.7%, p < 0.05). Angiotensinergic inhibition also reduced these parameters (-11.5 +/- 1.2 mmHg, -10.5 +/- 1.0%, p < 0.05) in 2K1C. In SHR, Kynurenic acid nanoinjections produced hypotension and sympathoinhibition (-21.0 +/- 2.5 mmHg, -24.7 +/- 2.4%, p < 0.05), as well losartan nanoinjections (-9.7 +/- 1.2 mmHg; p < 0.05) and RSNA (-12.0 +/- 2.4%, p < 0.05). These findings support the conclusion that a tonic excitatory neurotransmission exerted by angiotensin II, and mostly by glutamate in the MnPO could participate in the modulation of blood pressure and RSNA independent on whether hypertension is primarily neurogenic or is secondary to stenosis in renal artery.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
2019-10-04T12:31:45Z
2019-10-04T12:31:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.brainresbull.2018.06.011
Brain Research Bulletin. Oxford: Pergamon-elsevier Science Ltd, v. 142, p. 207-215, 2018.
0361-9230
http://hdl.handle.net/11449/184983
10.1016/j.brainresbull.2018.06.011
WOS:000448223400023
url http://dx.doi.org/10.1016/j.brainresbull.2018.06.011
http://hdl.handle.net/11449/184983
identifier_str_mv Brain Research Bulletin. Oxford: Pergamon-elsevier Science Ltd, v. 142, p. 207-215, 2018.
0361-9230
10.1016/j.brainresbull.2018.06.011
WOS:000448223400023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brain Research Bulletin
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 207-215
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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