Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.brainresbull.2018.06.011 http://hdl.handle.net/11449/184983 |
Resumo: | The crucial role of the median preoptic nucleus (MnPO) in the maintenance of hydroelectrolytic balance and autonomic regulation have been highlighted. Recently, the participation of the MnPO in the control of sympathetic nerve activity was demonstrated in essential hypertension model. However, peculiarities on the neurochemical changes underlying the differential role of MnPO during hypertension remain to be clarified. Therefore, this study aimed to investigate the main excitatory pathways that modulate MnPO neurons in hypertensive rats. Spontaneously hypertensive rats (SHR) and rats submitted previously to the Goldblatt protocol (two kidneys; one clip; 2K1C) were used. Rats of both groups (250 to 350 g, n = 6) were anesthetized with urethane (1.2 g/kg,i.v.) and instrumented to record mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Nanoinjection (100 nl) of saline (NaCl, 150 mM), losartan (AT1 receptor antagonist; 10 mM) and kynurenic acid (glutamate receptor antagonist; 50 mM) into the MnPO were performed. In 2K1C rats, glutamatergic blockade promoted decreases in MAP and RSNA (-19.1 +/- 0.9 mmHg, -21.6 +/- 2.8%, p < 0.05) when compared to saline (-0.4 +/- 0.6 mmHg, 0.2 +/- 0.7%, p < 0.05). Angiotensinergic inhibition also reduced these parameters (-11.5 +/- 1.2 mmHg, -10.5 +/- 1.0%, p < 0.05) in 2K1C. In SHR, Kynurenic acid nanoinjections produced hypotension and sympathoinhibition (-21.0 +/- 2.5 mmHg, -24.7 +/- 2.4%, p < 0.05), as well losartan nanoinjections (-9.7 +/- 1.2 mmHg; p < 0.05) and RSNA (-12.0 +/- 2.4%, p < 0.05). These findings support the conclusion that a tonic excitatory neurotransmission exerted by angiotensin II, and mostly by glutamate in the MnPO could participate in the modulation of blood pressure and RSNA independent on whether hypertension is primarily neurogenic or is secondary to stenosis in renal artery. |
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Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive stateMnPO2K1CSHRGlutamateAngiotensin IIThe crucial role of the median preoptic nucleus (MnPO) in the maintenance of hydroelectrolytic balance and autonomic regulation have been highlighted. Recently, the participation of the MnPO in the control of sympathetic nerve activity was demonstrated in essential hypertension model. However, peculiarities on the neurochemical changes underlying the differential role of MnPO during hypertension remain to be clarified. Therefore, this study aimed to investigate the main excitatory pathways that modulate MnPO neurons in hypertensive rats. Spontaneously hypertensive rats (SHR) and rats submitted previously to the Goldblatt protocol (two kidneys; one clip; 2K1C) were used. Rats of both groups (250 to 350 g, n = 6) were anesthetized with urethane (1.2 g/kg,i.v.) and instrumented to record mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Nanoinjection (100 nl) of saline (NaCl, 150 mM), losartan (AT1 receptor antagonist; 10 mM) and kynurenic acid (glutamate receptor antagonist; 50 mM) into the MnPO were performed. In 2K1C rats, glutamatergic blockade promoted decreases in MAP and RSNA (-19.1 +/- 0.9 mmHg, -21.6 +/- 2.8%, p < 0.05) when compared to saline (-0.4 +/- 0.6 mmHg, 0.2 +/- 0.7%, p < 0.05). Angiotensinergic inhibition also reduced these parameters (-11.5 +/- 1.2 mmHg, -10.5 +/- 1.0%, p < 0.05) in 2K1C. In SHR, Kynurenic acid nanoinjections produced hypotension and sympathoinhibition (-21.0 +/- 2.5 mmHg, -24.7 +/- 2.4%, p < 0.05), as well losartan nanoinjections (-9.7 +/- 1.2 mmHg; p < 0.05) and RSNA (-12.0 +/- 2.4%, p < 0.05). These findings support the conclusion that a tonic excitatory neurotransmission exerted by angiotensin II, and mostly by glutamate in the MnPO could participate in the modulation of blood pressure and RSNA independent on whether hypertension is primarily neurogenic or is secondary to stenosis in renal artery.Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Biol Sci Inst, Dept Physiol Sci, Ctr Neurosci & Cardiovasc Res, Goiania, Go, BrazilUniv Fed Goias, Biol Sci Inst, Dept Physiol Sci, Lab Cardiovasc Physiol & Therapeut, Goiania, Go, BrazilPontifical Catholic Univ Goias, Sch Med Pharm & Biomed, Goiania, Go, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilFundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2012/0055431086Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2009/10267000352CNPq: 483411/2012-4CNPq: 447496/2014-0Elsevier B.V.Universidade Federal de Goiás (UFG)Pontifical Catholic Univ GoiasUniversidade Estadual Paulista (Unesp)Mourao, Aline A.Soares de Mello, Aryanne B.Dos Santos Moreira, Marina C.Rodrigues, Karla L.Lopes, Paulo R.Xavier, Carlos H.Gomes, Rodrigo M.Freiria-Oliveira, Andre H.Blanch, Graziela T.Colombari, Eduardo [UNESP]Pedrino, Gustavo R.2019-10-04T12:31:45Z2019-10-04T12:31:45Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article207-215http://dx.doi.org/10.1016/j.brainresbull.2018.06.011Brain Research Bulletin. Oxford: Pergamon-elsevier Science Ltd, v. 142, p. 207-215, 2018.0361-9230http://hdl.handle.net/11449/18498310.1016/j.brainresbull.2018.06.011WOS:000448223400023Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Research Bulletininfo:eu-repo/semantics/openAccess2024-09-27T14:04:57Zoai:repositorio.unesp.br:11449/184983Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:04:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
title |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
spellingShingle |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state Mourao, Aline A. MnPO 2K1C SHR Glutamate Angiotensin II |
title_short |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
title_full |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
title_fullStr |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
title_full_unstemmed |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
title_sort |
Median preoptic nucleus excitatory neurotransmitters in the maintenance of hypertensive state |
author |
Mourao, Aline A. |
author_facet |
Mourao, Aline A. Soares de Mello, Aryanne B. Dos Santos Moreira, Marina C. Rodrigues, Karla L. Lopes, Paulo R. Xavier, Carlos H. Gomes, Rodrigo M. Freiria-Oliveira, Andre H. Blanch, Graziela T. Colombari, Eduardo [UNESP] Pedrino, Gustavo R. |
author_role |
author |
author2 |
Soares de Mello, Aryanne B. Dos Santos Moreira, Marina C. Rodrigues, Karla L. Lopes, Paulo R. Xavier, Carlos H. Gomes, Rodrigo M. Freiria-Oliveira, Andre H. Blanch, Graziela T. Colombari, Eduardo [UNESP] Pedrino, Gustavo R. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Pontifical Catholic Univ Goias Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mourao, Aline A. Soares de Mello, Aryanne B. Dos Santos Moreira, Marina C. Rodrigues, Karla L. Lopes, Paulo R. Xavier, Carlos H. Gomes, Rodrigo M. Freiria-Oliveira, Andre H. Blanch, Graziela T. Colombari, Eduardo [UNESP] Pedrino, Gustavo R. |
dc.subject.por.fl_str_mv |
MnPO 2K1C SHR Glutamate Angiotensin II |
topic |
MnPO 2K1C SHR Glutamate Angiotensin II |
description |
The crucial role of the median preoptic nucleus (MnPO) in the maintenance of hydroelectrolytic balance and autonomic regulation have been highlighted. Recently, the participation of the MnPO in the control of sympathetic nerve activity was demonstrated in essential hypertension model. However, peculiarities on the neurochemical changes underlying the differential role of MnPO during hypertension remain to be clarified. Therefore, this study aimed to investigate the main excitatory pathways that modulate MnPO neurons in hypertensive rats. Spontaneously hypertensive rats (SHR) and rats submitted previously to the Goldblatt protocol (two kidneys; one clip; 2K1C) were used. Rats of both groups (250 to 350 g, n = 6) were anesthetized with urethane (1.2 g/kg,i.v.) and instrumented to record mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Nanoinjection (100 nl) of saline (NaCl, 150 mM), losartan (AT1 receptor antagonist; 10 mM) and kynurenic acid (glutamate receptor antagonist; 50 mM) into the MnPO were performed. In 2K1C rats, glutamatergic blockade promoted decreases in MAP and RSNA (-19.1 +/- 0.9 mmHg, -21.6 +/- 2.8%, p < 0.05) when compared to saline (-0.4 +/- 0.6 mmHg, 0.2 +/- 0.7%, p < 0.05). Angiotensinergic inhibition also reduced these parameters (-11.5 +/- 1.2 mmHg, -10.5 +/- 1.0%, p < 0.05) in 2K1C. In SHR, Kynurenic acid nanoinjections produced hypotension and sympathoinhibition (-21.0 +/- 2.5 mmHg, -24.7 +/- 2.4%, p < 0.05), as well losartan nanoinjections (-9.7 +/- 1.2 mmHg; p < 0.05) and RSNA (-12.0 +/- 2.4%, p < 0.05). These findings support the conclusion that a tonic excitatory neurotransmission exerted by angiotensin II, and mostly by glutamate in the MnPO could participate in the modulation of blood pressure and RSNA independent on whether hypertension is primarily neurogenic or is secondary to stenosis in renal artery. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-01 2019-10-04T12:31:45Z 2019-10-04T12:31:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.brainresbull.2018.06.011 Brain Research Bulletin. Oxford: Pergamon-elsevier Science Ltd, v. 142, p. 207-215, 2018. 0361-9230 http://hdl.handle.net/11449/184983 10.1016/j.brainresbull.2018.06.011 WOS:000448223400023 |
url |
http://dx.doi.org/10.1016/j.brainresbull.2018.06.011 http://hdl.handle.net/11449/184983 |
identifier_str_mv |
Brain Research Bulletin. Oxford: Pergamon-elsevier Science Ltd, v. 142, p. 207-215, 2018. 0361-9230 10.1016/j.brainresbull.2018.06.011 WOS:000448223400023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brain Research Bulletin |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
207-215 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546424039636992 |