Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.hindawi.com/journals/mi/2015/481972/ http://hdl.handle.net/11449/128792 |
Resumo: | Objective. Helicobacter pylori (Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response. Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression in H. pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment. Methods. A total of 37 patients CG-Hp+ were evaluated. The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry. Results. Before treatment both TLR2 and TLR4 mRNA in CG-Hp+ patients were slightly increased (TLR2 = 1.32; TLR4 = 1.26) in relation to Hp-negative normal gastric mucosa (P <= 0.05). After successful eradication therapy no significant change was observed (TLR2 = 1.47; TLR4 = 1.53; P > 0.05). In addition, the cagA and vacA bacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values of TLR2 and TLR4, both before and after treatment. Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results. Conclusion. In conclusion, the expression of both TLR2 and TLR4 is increased in CG-Hp+ patients regardless of cagA and vacA status and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated. |
id |
UNSP_9d333d4adf1166f34e0217d492bd492e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/128792 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric LesionsObjective. Helicobacter pylori (Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response. Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression in H. pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment. Methods. A total of 37 patients CG-Hp+ were evaluated. The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry. Results. Before treatment both TLR2 and TLR4 mRNA in CG-Hp+ patients were slightly increased (TLR2 = 1.32; TLR4 = 1.26) in relation to Hp-negative normal gastric mucosa (P <= 0.05). After successful eradication therapy no significant change was observed (TLR2 = 1.47; TLR4 = 1.53; P > 0.05). In addition, the cagA and vacA bacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values of TLR2 and TLR4, both before and after treatment. Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results. Conclusion. In conclusion, the expression of both TLR2 and TLR4 is increased in CG-Hp+ patients regardless of cagA and vacA status and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilAmbulatory Gastrohepatol, Base Hosp, BR-15090000 Sao Jose Do Rio Preto, SP, BrazilJoao Paulo II Hosp, BR-15030070 Sao Jose Do Rio Preto, SP, BrazilUniv Sao Paulo, Sch Med, BR-01246903 Sao Paulo, SP, BrazilFAMERP, Sch Med, BR-15090000 Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilFAPESP: 2012/15036-8CNPq: 304870/2012-9Hindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Ambulatory GastrohepatolJoao Paulo II HospUniversidade de São Paulo (USP)Targa Cadamuro, Aline Cristina [UNESP]Teixeira Rossi, Ana Flavia [UNESP]Biselli-Perico, Joice Matos [UNESP]Pereira, Patricia FucutaBedin Mascarin Do Vale, Edla PolsinelliAcayaba, RicardoMoreira Leite, Ktia RamosGoloni-Bertollo, Eny MariaSilva, Ana Elizabete [UNESP]2015-10-21T13:13:37Z2015-10-21T13:13:37Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://www.hindawi.com/journals/mi/2015/481972/Mediators Of Inflammation, p. 9, 2015.0962-9351http://hdl.handle.net/11449/12879210.1155/2015/481972WOS:000352445200001WOS000352445200001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators Of Inflammation3.5491,370info:eu-repo/semantics/openAccess2024-01-29T06:31:26Zoai:repositorio.unesp.br:11449/128792Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:14:28.757397Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
title |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
spellingShingle |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions Targa Cadamuro, Aline Cristina [UNESP] |
title_short |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
title_full |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
title_fullStr |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
title_full_unstemmed |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
title_sort |
Effect of helicobacter pylori eradication on TLR2 and TLR4 expression in patients with Gastric Lesions |
author |
Targa Cadamuro, Aline Cristina [UNESP] |
author_facet |
Targa Cadamuro, Aline Cristina [UNESP] Teixeira Rossi, Ana Flavia [UNESP] Biselli-Perico, Joice Matos [UNESP] Pereira, Patricia Fucuta Bedin Mascarin Do Vale, Edla Polsinelli Acayaba, Ricardo Moreira Leite, Ktia Ramos Goloni-Bertollo, Eny Maria Silva, Ana Elizabete [UNESP] |
author_role |
author |
author2 |
Teixeira Rossi, Ana Flavia [UNESP] Biselli-Perico, Joice Matos [UNESP] Pereira, Patricia Fucuta Bedin Mascarin Do Vale, Edla Polsinelli Acayaba, Ricardo Moreira Leite, Ktia Ramos Goloni-Bertollo, Eny Maria Silva, Ana Elizabete [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ambulatory Gastrohepatol Joao Paulo II Hosp Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Targa Cadamuro, Aline Cristina [UNESP] Teixeira Rossi, Ana Flavia [UNESP] Biselli-Perico, Joice Matos [UNESP] Pereira, Patricia Fucuta Bedin Mascarin Do Vale, Edla Polsinelli Acayaba, Ricardo Moreira Leite, Ktia Ramos Goloni-Bertollo, Eny Maria Silva, Ana Elizabete [UNESP] |
description |
Objective. Helicobacter pylori (Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response. Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression in H. pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment. Methods. A total of 37 patients CG-Hp+ were evaluated. The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry. Results. Before treatment both TLR2 and TLR4 mRNA in CG-Hp+ patients were slightly increased (TLR2 = 1.32; TLR4 = 1.26) in relation to Hp-negative normal gastric mucosa (P <= 0.05). After successful eradication therapy no significant change was observed (TLR2 = 1.47; TLR4 = 1.53; P > 0.05). In addition, the cagA and vacA bacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values of TLR2 and TLR4, both before and after treatment. Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results. Conclusion. In conclusion, the expression of both TLR2 and TLR4 is increased in CG-Hp+ patients regardless of cagA and vacA status and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:13:37Z 2015-10-21T13:13:37Z 2015-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.hindawi.com/journals/mi/2015/481972/ Mediators Of Inflammation, p. 9, 2015. 0962-9351 http://hdl.handle.net/11449/128792 10.1155/2015/481972 WOS:000352445200001 WOS000352445200001.pdf |
url |
http://www.hindawi.com/journals/mi/2015/481972/ http://hdl.handle.net/11449/128792 |
identifier_str_mv |
Mediators Of Inflammation, p. 9, 2015. 0962-9351 10.1155/2015/481972 WOS:000352445200001 WOS000352445200001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators Of Inflammation 3.549 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129599205277696 |