Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?

Detalhes bibliográficos
Autor(a) principal: Ribeiro do Amaral, Renata Claro
Data de Publicação: 2020
Outros Autores: Caleffi-Ferracioli, Katiany Rizzieri, Demitto, Fernanda de Oliveira, Almeida, Aryadne Larissa de, Dias Siqueira, Vera Lucia, Lima Scodro, Regiane Bertin de, Fujimura Leite, Clarice Queico [UNESP], Pavan, Fernando Rogerio [UNESP], Cardoso, Rosilene Fressatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/s2175-97902020000218309
http://hdl.handle.net/11449/210553
Resumo: The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP combination showed no significant change in the Minimum inhibitory concentration (M IC) values of INH (FIC >= 0.5; MF=1 or 2).The use of VP in tuberculosis therapy should be managed carefully, considering the resistance caused by specific mutation in katG and inhA genes, in which the use of these EPIs may have no success. The use of EPIs as an adjunctive drug in the anti-tuberculosis therapy should be further investigated on a larger number of M. tuberculosis clinical isolates with different resistant profile.
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spelling Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?TuberculosisMultidrug-resistanceEfflux pumpsEfflux pumps inhibitorsisoniazidThe membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP combination showed no significant change in the Minimum inhibitory concentration (M IC) values of INH (FIC >= 0.5; MF=1 or 2).The use of VP in tuberculosis therapy should be managed carefully, considering the resistance caused by specific mutation in katG and inhA genes, in which the use of these EPIs may have no success. The use of EPIs as an adjunctive drug in the anti-tuberculosis therapy should be further investigated on a larger number of M. tuberculosis clinical isolates with different resistant profile.Univ Estadual Maringa, Biosci & Physiopathol, Maringa, Parana, BrazilUniv Estadual Maringa, Hlth Sci, Maringa, Parana, BrazilUniv Estadual Maringa, Dept Clin Anal & Biomed, Lab Med Bacteriol, Maringa, Parana, BrazilUniv Estadual Paulista, Sch Pharmaceut Sci, Lab Mycobacteriol Prof Dr Hugo David, Araraquara, SP, BrazilUniv Estadual Paulista, Sch Pharmaceut Sci, Lab Mycobacteriol Prof Dr Hugo David, Araraquara, SP, BrazilUniv Sao Paulo, Conjunto QuimicasUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Ribeiro do Amaral, Renata ClaroCaleffi-Ferracioli, Katiany RizzieriDemitto, Fernanda de OliveiraAlmeida, Aryadne Larissa deDias Siqueira, Vera LuciaLima Scodro, Regiane Bertin deFujimura Leite, Clarice Queico [UNESP]Pavan, Fernando Rogerio [UNESP]Cardoso, Rosilene Fressatti2021-06-25T21:07:57Z2021-06-25T21:07:57Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1590/s2175-97902020000218309Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo: Univ Sao Paulo, Conjunto Quimicas, v. 56, 7 p., 2020.1984-8250http://hdl.handle.net/11449/21055310.1590/s2175-97902020000218309WOS:000592261300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Pharmaceutical Sciencesinfo:eu-repo/semantics/openAccess2024-06-24T13:07:13Zoai:repositorio.unesp.br:11449/210553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:05:44.035088Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
title Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
spellingShingle Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
Ribeiro do Amaral, Renata Claro
Tuberculosis
Multidrug-resistance
Efflux pumps
Efflux pumps inhibitors
isoniazid
title_short Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
title_full Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
title_fullStr Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
title_full_unstemmed Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
title_sort Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
author Ribeiro do Amaral, Renata Claro
author_facet Ribeiro do Amaral, Renata Claro
Caleffi-Ferracioli, Katiany Rizzieri
Demitto, Fernanda de Oliveira
Almeida, Aryadne Larissa de
Dias Siqueira, Vera Lucia
Lima Scodro, Regiane Bertin de
Fujimura Leite, Clarice Queico [UNESP]
Pavan, Fernando Rogerio [UNESP]
Cardoso, Rosilene Fressatti
author_role author
author2 Caleffi-Ferracioli, Katiany Rizzieri
Demitto, Fernanda de Oliveira
Almeida, Aryadne Larissa de
Dias Siqueira, Vera Lucia
Lima Scodro, Regiane Bertin de
Fujimura Leite, Clarice Queico [UNESP]
Pavan, Fernando Rogerio [UNESP]
Cardoso, Rosilene Fressatti
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ribeiro do Amaral, Renata Claro
Caleffi-Ferracioli, Katiany Rizzieri
Demitto, Fernanda de Oliveira
Almeida, Aryadne Larissa de
Dias Siqueira, Vera Lucia
Lima Scodro, Regiane Bertin de
Fujimura Leite, Clarice Queico [UNESP]
Pavan, Fernando Rogerio [UNESP]
Cardoso, Rosilene Fressatti
dc.subject.por.fl_str_mv Tuberculosis
Multidrug-resistance
Efflux pumps
Efflux pumps inhibitors
isoniazid
topic Tuberculosis
Multidrug-resistance
Efflux pumps
Efflux pumps inhibitors
isoniazid
description The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP combination showed no significant change in the Minimum inhibitory concentration (M IC) values of INH (FIC >= 0.5; MF=1 or 2).The use of VP in tuberculosis therapy should be managed carefully, considering the resistance caused by specific mutation in katG and inhA genes, in which the use of these EPIs may have no success. The use of EPIs as an adjunctive drug in the anti-tuberculosis therapy should be further investigated on a larger number of M. tuberculosis clinical isolates with different resistant profile.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
2021-06-25T21:07:57Z
2021-06-25T21:07:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/s2175-97902020000218309
Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo: Univ Sao Paulo, Conjunto Quimicas, v. 56, 7 p., 2020.
1984-8250
http://hdl.handle.net/11449/210553
10.1590/s2175-97902020000218309
WOS:000592261300001
url http://dx.doi.org/10.1590/s2175-97902020000218309
http://hdl.handle.net/11449/210553
identifier_str_mv Brazilian Journal Of Pharmaceutical Sciences. Sao Paulo: Univ Sao Paulo, Conjunto Quimicas, v. 56, 7 p., 2020.
1984-8250
10.1590/s2175-97902020000218309
WOS:000592261300001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Pharmaceutical Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
dc.publisher.none.fl_str_mv Univ Sao Paulo, Conjunto Quimicas
publisher.none.fl_str_mv Univ Sao Paulo, Conjunto Quimicas
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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